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VIP (NM_194435) Human Untagged Clone
CAT＃: SC126753
VIP (untagged)-Human vasoactive intestinal peptide (VIP), transcript variant 2
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PHM27 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_194435, the custom clone sequence may differ by one or more nucleotides
ATGGACACCAGAAATAAGGCCCAGCTCCTTGTGCTCCTGACTCTTCTCAGTGTGCTCTTCTCACAGACTT CGGCATGGCCTCTTTACAGGGCACCTTCTGCTCTCAGGTTGGGTGACAGAATACCCTTTGAGGGAGCAAA TGAACCTGATCAAGTTTCATTAAAAGAAGACATTGACATGTTGCAAAATGCATTAGCTGAAAATGACACA CCCTATTATGATGTATCCAGAAATGCCAGGCATGCTGATGGAGTTTTCACCAGTGACTTCAGTAAACTCT TGGGTCAACTTTCTGCCAAAAAGTACCTTGAGTCTCTTATGGGAAAACGTGTTAGTAACATCTCAGAAGA CCCTGTACCAGTCAAACGTCACTCAGATGCAGTCTTCACTGACAACTATACCCGCCTTAGAAAACAAATG GCTGTAAAGAAATATTTGAACTCAATTCTGAATGGAAAGAGGAGCAGTGAGGGAGAATCTCCCGACTTTC CAGAAGAGTTAGAAAAATGA >OriGene 5' read for NM_194435 unedited
ATACGACTACTATAGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGA GGGCCAACCCTTAGCCATTGCTAAGGGCAGAGAACTGGTGGAGCCTTTCTCTTACTCCCA GGACTTCAGCACCTAAGACAGCTCCAAAACAAACCAGAACAGTCAGCTCCGGGGGAGCAC GACTGGGCGAGAGGCACAGAAATGGACACCAGAAATAAGGCCCAGCTCCTTGTGCTCCTG ACTCTTCTCAGTGTGCTCTTCTCACAGACTTCGGCATGGCCTCTTTACAGGGCACCTTCT GCTCTCAGGTTGGGTGACAGAATACCCTTTGAGGGAGCAAATGAACCTGATCAAGTTTCA TTAAAAGAAGACATTGACATGTTGCAAAATGCATTAGCTGAAAATGACACACCCTATTAT GATGTATCCAGAAATGCCAGGCATGCTGATGGAGTTTTCACCAGTGACTTCAGTAAACTC TTGGGTCAACTTTCTGCCAAAAAGTACCTTGAGTCTCTTATGGGAAAACGTNGTAGCAGT AACATCTCAGAAGACCCTGTACCAGTCAAACGTCACTCAGATGCAGTCTTCACTGACAAC TATACCCGCCTTAGAAAACANATGGCTGTANAGAAATATTTGCACTCATTTCTGAATGGA AAGAGGAGCAGTGAGGGGAGAATCTCCCGACTTTCCAGAAGAGTTAGAAGATGATGAAAA CGACCTTTGGAGCCGAGCTGATGACACCTTCCAGTGCACTCTTGAGGGACACGATCGCAC ATATTAATTTTGAGTCTACCTAAGTATCAAGAGACAACTTCATATCAAACCAATAAAAAT TGGGGTGTGAAGTGTGATGTTTTACCTAAGAATACCTGGAGCTACATGTAATAGTTTGGG AGTCTAATTTGGCTTACTATAAAAGCGGCATTCAATGCGGTATCCTTTCACACAAATATT TCAGAAAGGAAGGCTGGTATTCATTTGAGAAGTCTGAAGG >OriGene 3' read for NM_194435 unedited
CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTATGGCAAAAATATTTAT TATTGAACAACTGAGTGTTTCATTCACTTCACATTTTACACATACTTAACACAAGCAGAA AGGGAAGTTTCAGTGTCAGAAGCAGGGGTCGTTCTCCTTAACTGAAAGACTATCAGGAGA GAGCCTTTGGTGATTAGCAATCCAAATTTGAGATTTTTTAAAACACCATTAAAAATAAAG GTTGCCTATAAAATATGGAAACACATAATATTTTCTGAAAATCTTTTTGGAAGAATGTCT TTTTATCTTCATTGTTACTATCTTAGACATTTTGAGGTTTATTTAACACAGCATTCCATC TGCTTAACATTCAAATTTAGACACACTGATTATCTGTTCTACTCTCCTGAATAGTTAAGT ACACTCTATAAATAAACACATCAATTTTGCTCTATTACTACTCTTCCAAAAACGTCTCAT ATAATTGGAATTAACCTAGCATTTACTTATCATTAAATATATTTTTTTGTTAGAAAGGAT ATACAGCATATGAAATTGCAGGCTTTTTATGAGTTAAAGACAAAATTTAGAACTCTCAAA TACTATTTACAATGTAAACTTCACAGTTATTACATTAGCTAGAATACATCACAACATTCA CAACACAATATTTTTATTTGGTTTGGATATTGAAGTTGTTTTCTTGAATTACTTATGTAG AACTCAAAATTTAATTATGTTGCGTATCGTTTTCCTTCAAGAATTCACTGGNGAAGTGTC ATCAGCTTTGCTCCAAGGTCTTTTTATCATTTTCTAACTCTTCTGGAAGTCGGGAGATCT NCCTCACTGCTCTCTTTCATTCAGAAATGAGTCAATATTNCTTAACGCCATTGTTTCTAA GCGGGTATAGTGCAGTGAGACTGCTCTGATGACGTTGACTGTACGGGNTTCTGAGAGTAC TGCTACCGTTTCCTAAGAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_194435 |
| Insert Size | 1470 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_194435.1, NP_919416.1 |
| RefSeq Size | 1615 bp |
| RefSeq ORF | 510 bp |
| Locus ID | 7432 |
| UniProt ID | P01282 |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| Gene Summary | The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Opposing Effects of Two Tissue Transglutaminase Protein Isoforms in Neuroblastoma Cell Differentiation
,Andrew E. L. Tee, Glenn M. Marshall, Pei Y. Liu, Ning Xu, Michelle Haber, Murray D. Norris, Siiri E. Iismaa, and Tao Liu,
J. Biol. Chem., Feb 2010; 285: 3561 - 3567
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