United States
Germany
Japan
United Kingdom
China
MID1 (NM_033290) Human Untagged Clone
CAT＃: SC126750
MID1 (untagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 3
CNY 4,896.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BBBG1; FXY; GBBB1; MIDIN; OGS1; OS; OSX; RNF59; TRIM18; XPRF; ZNFXY |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126750 sequence for NM_033290 edited (data generated by NextGen Sequencing)
ATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTTGAGGACCCT CTTCTACTGCCCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATCCTAGTATCA CACTGTGCCACCAACGAGTCTGTGGAGTCCATCACCGCCTTCCAGTGCCCCACCTGCCGG CATGTCATCACCCTCAGCCAGCGAGGTCTAGACGGGCTCAAGCGCAACGTCACCCTACAG AACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGCCCAACTCTCCCAGCGAGACC CGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGTCCTCTGCCAG TTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGTGAAGTATCC TACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACAGGCCATCGT CTGATTGAGCCAATTCCGGACTCTCACATCCGGGGGCTGATGTGCTTGGAGCATGAGGAT GAGAAGGTGAATATGTACTGTGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTG GTTGGGCGGCACCGCGATCATCAGGTGGCAGCTTTGAGTGAGCGCTATGACAAATTGAAG CAAAACTTAGAGAGTAACCTCACCAACCTTATTAAGAGGAACACAGAACTGGAGACCCTT TTGGCTAAACTCATCCAAACCTGTCAACATGTTGAAGTCAATGCATCACGTCAAGAAGCC AAATTGACAGAGGAGTGTGATCTTCTCATTGAGATCATTCAGCAAAGACGACAGATTATT GGAACCAAGATCAAAGAAGGGAAGGTGATGAGGCTTCGCAAACTGGCTCAGCAGATTGCA AACTGCAAACAGTGCATTGAGCGGTCAGCATCACTCATCTCCCAAGCGGAACACTCTCTG AAGGAGAATGATCATGCGCGTTTCCTACAGACTGCTAAGAATATCACCGAGAGAGTCTCC ATGGCAACTGCATCCTCCCAGGTTCTAATTCCTGAAATCAACCTCAATGACACATTTGAC ACCTTTGCCTTAGATTTTTCCCGAGAGAAGAAACTGCTAGAATGTCTGGATTACCTTACA GCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACAGCTTCATATGACACCATCACT GTGCATTGGACCTCCGATGATGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA TTCACCGGACAAGCCAACGTCGTTAGTCTGTGTAATTCGGCTGATAGCTGGATGATAGTA CCCAACATCAAGCAGAACCACTACACGGTGCACGGTCTGCAGAGCGGCACCAAGTACATC TTCATGGTCAAGGCCATCAACCAGGCGGGCAGCCGCAGCAGTGAGCCTGGGAAGTTGAAG ACAAACAGCCAACCATTTAAACTGGATCCCAAATCTGCTCATCGAAAACTGAAGGTGTCC CATGATAACTTGACAGTAGAACGTGATGAGTCATCATCCAAGAAGAGTCACACACCTGAA CGCTTCACCAGCCAGGGGAGCTATGGAGTAGCTGGAAATGTGTTTATTGATAGTGGCCGG CATTATTGGGAAGTGGTCATAAGTGGAAGCACATGGTATGCCATTGGTCTTGCTTACAAA TCAGCCCCGAAGCATGAATGGATTGGGAAGAACTCTGCTTCCTGGGCGCTCTGCCGCTGC AACAATAACTGGGTGGTGAGACACAATAGCAAGGAAATCCCCATTGAGCCTGCCCCCCAC CTCCGGCGCGTGGGCATCCTGCTGGACTATGATAACGGCTCTATCGCCTTTTATGATGCT TTGAACTCCATCCACCTCTACACCTTCGACGTCGCATTTGCGCAGCCTGTTTGCCCCACC TTCACCGTGTGGAACAAGTGTCTGACGATTATCACTGGGCTCCCTATCCCAGACCATTTG GACTGCACAGAGCAGCTGCCGTGA Clone variation with respect to NM_033290.3 >OriGene 5' read for NM_033290 unedited
GTTCGGATTATGTAATACGACTCACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGGTT AGTATGTAGTGGAGCTGGTGGTGTGAAGCTGATTCATTTTGTTTCAAAGCCCCAAAGGCA TGCTGCCGAACACAGGCACAAGCACAGCAGCGTCTCCTTAGAAATAATGACTCCAAGGCA AACAGCCCTCATTGCAAAATGTAGAAGACTGCCATCTGCTTGTCTCCACTGTGGTTTATT CGTTTCAGGGTTTTAAATGACGTAATAAAAAGATAGCTGATCAGCTTCCTTGGGTTTTGC TGATGACACAAGAGAGCTTTGCCTGAAGATGGAAACACTGGAGTCAGAACTGACCTGCCC TATTTGTCTGGAGCTCTTTGAGGACCCTCTTCTACTGCCCTGCGCACACAGCCTCTGCTT CAACTGCGCCCACCGCATCCTAGTATCACACTGTGCCACCAACGAGTCTGTGGAGTCCAT CACCGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTAGA CGGGCTCAAGCGCAACGTCACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGT GAGCGGGCCCAACTCTCCCAGCGAGACCCGTCGGAAGCGGGCCTTTGACGCCAACACCAT GACCTCCGCCGAGAAGGTCTCTGCCAGTTTTTGACCCAGATCCTGCCCAGACGCTGTGAA GACCTGTGTCACTTGTGAAATATCCTACTGTGACGAGTGCCTGAAAGCACTCACCCCAAT AAGAACCCCTTACAGTCCATTGGCTGATGGAGCCATTCCCGGACCTCCCATACGGGGGCT GAAGTGCCTGGACCCTGAGGATGAGTAGCCAAACTTAAAGAGAAACCCCCCCACCTTATT AAAAGAAACCAAAACCGTGGACCCTTTGG >OriGene 3' read for NM_033290 unedited
GCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTCTTACAACTCTATTGT AAACTATACTAGACTATAGAGGGACTTCTACATCTTTCAAGATGTGTTTAATAAAGGTCT GTTTATAATAACTTTTGAGGCATGAATCTAGCAAATAGTACTTTATACAATGTCCCTTGT CATTACCAACTCATAAATATTAAGTGTTTTTCAGTGACTTATGTTTGGATGTGGTAGTGC TGATCAAGGCCATGTGCTGATGTCCTGGAGAGCAAAATCAATCCAAAGTGGTGCTGCTAT TTGTGACAGAACATGTTTATTTACTCAGCCCCGGAGACAAAAGGAAAATTGATATGGGGG AGCGGGAAATAGGAGAACTATTAAATGTAGTGAAGAAATTTCACAGGTCTAAAGGAACTA TTAAAAGGAAGGATAAAGTAGATTCTATACTATAAAACAGAATCCTACCTCTGATAAAAG ACAAATCAGCCTGAATTTTTGAATAATCAATAGGATTCAAAATGACTATTTTCAATTGCA ATCTCATTCTTAATGTTCTTCCAGAACCCTTAACCCTGAGAGAAGCAGGGCAAAATGTGG CCAAAGGATTCAGAACCTTTCTTTCCAACATTATCCCATCAGCTATGAGAAAGTAAAGAT TTAGCCACGGCAAGGTCCGAAGTCCAAAATCAACTCTTTTCATTCTCTGTCTTGCTCTCC ATTTTAGTTGATCTGATACACCCTTTTGACATCACAATTAAAACACTTCAAATTAAACCA GAGTTAGAAAGCCTGTTTCCTCCCCTACTAAGTTCAACACATCCTGTTTTGCCCCATCGA GTGAGAAGACCTCCCTGGGATCTCATGGGATTCACGTAGAAAAGAGTACATCACAACTCC AAGTTTTGTTTTTTGTAGCCCCAGATGGGAGAAATGAAGTGNAATACTGAAAACTATGGC CCCTN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_033290 |
| Insert Size | 3570 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033290.1, NP_150632.1 |
| RefSeq Size | 3117 bp |
| RefSeq ORF | 2004 bp |
| Locus ID | 4281 |
| UniProt ID | O15344 |
| Domains | zf-B_box, RING, BBC, SPRY, FN3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016] Transcript Variant: This variant (3) has an alternate 5' UTR exon, as compared to variant 1. Variants 1-4 and variant 10 encode the same isoform (1). Expression was found to be adipose specific. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211781 | MID1 (Myc-DDK-tagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 3 |
CNY 4,888.00 |
|
| RC211781L3 | Lenti ORF clone of Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211781L4 | Lenti ORF clone of Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG211781 | MID1 (tGFP-tagged) - Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 3 |
CNY 6,488.00 |
