United States
Germany
Japan
United Kingdom
China
SHC (SHC1) (NM_003029) Human Untagged Clone
CAT＃: SC126613
SHC1 (untagged)-Human SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2
CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SHC; SHCA |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_003029 edited
GGGGAGAAAGTTGGAGCGGTAACCTAAGCTGGCAGTGGCGTGATCCGGCACCAAATCGGC CCGCGGTGCGGTGCGGAGACTCCATGAGGCCCTGGACATGAACAAGCTGAGTGGAGGCGG CGGGCGCAGGACTCGGGTGGAAGGGGGCCAGCTTGGGGGCGAGGAGTGGACCCGCCACGG GAGCTTTGTCAATAAGCCCACGCGGGGCTGGCTGCATCCCAACGACAAAGTCATGGGACC CGGGGTTTCCTACTTGGTTCGGTACATGGGTTGTGTGGAGGTCCTCCAGTCAATGCGTGC CCTGGACTTCAACACCCGGACTCAGGTCACCAGGGAGGCCATCAGTCTGGTGTGTGAGGC TGTGCCGGGTGCTAAGGGGGCGACAAGGAGGAGAAAGCCCTGTAGCCGCCCGCTCAGCTC TATCCTGGGGAGGAGTAACCTGAAATTTGCTGGAATGCCAATCACTCTCACCGTCTCCAC CAGCAGCCTCAACCTCATGGCCGCAGACTGCAAACAGATCATCGCCAACCACCACATGCA ATCTATCTCATTTGCATCCGGCGGGGATCCGGACACAGCCGAGTATGTCGCCTATGTTGC CAAAGACCCTGTGAATCAGAGAGCCTGCCACATTCTGGAGTGTCCCGAAGGGCTTGCCCA GGATGTCATCAGCACCATTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATACCTCAGGAA CCCACCCAAACTGGTCACCCCTCATGACAGGATGGCTGGCTTTGATGGCTCAGCATGGGA TGAGGAGGAGGAAGAGCCACCTGACCATCAGTACTATAATGACTTCCCGGGGAAGGAACC CCCCTTGGGGGGGGTGGTAGACATGAGGCTTCGGGAAGGAGCCGCTCCAGGGGCTGCTCG ACCCACTGCACCCAATGCCCAGACCCCCAGCCACTTGGGAGCTACATTGCCTGTAGGACA GCCTGTTGGGGGAGATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACCCTGTCCAGG CAGAGAGCTTTTTGATGATCCCTCCTATGTCAACGTCCAGAACCTAGACAAGGCCCGGCA AGCAGTGGGTGGTGCTGGGCCCCCCAATCCTGCTATCAATGGCAGTGCACCCCGGGACCT GTTTGACATGAAGCCCTTCGAAGATGCTCTTCGCGTGCCTCCACCTCCCCAGTCGGTGTC CATGGCTGAGCAGCTCCGAGGGGAGCCCTGGTTCCATGGGAAGCTGAGCCGGCGGGAGGC TGAGGCACTGCTGCAGCTCAATGGGGACTTCCTGGTACGGGAGAGCACGACCACACCTGG CCAGTATGTGCTCACTGGCTTGCAGAGTGGGCAGCCTAAGCATTTGCTACTGGTGGACCC TGAGGGTGTGGTTCGGACTAAGGATCACCGCTTTGAAAGTGTCAGTCACCTTATCAGCTA CCACATGGACAATCACTTGCCCATCATCTCTGCGGGCAGCGAACTGTGTCTACAGCAACC TGTGGAGCGGAAACTGTGATCTGCCCTAGCGCTCTCTTCCAGAAGATGCCCTCCAATCCT TTCCACCCTATTCCCTAACTCTCGGGACCTCGTTTGGGAGTGTTCTGTGGGCTTGGCCTT GTGTCAGAGCTGGGAGTAGCATGGACTCTGGGTTTCATATCCAGCTGAGTGAGAGGGTTT GAGTCAAAAGCCTGGGTGAGAATCCTGCCTCTCCCCAAACATTAATCACCAAAGTATTAA TGTACAGAGTGGCCCCTCACCTGGGCCTTTCCTGTGCCAACCTGATGCCCCTTCCCCAAG AAGGTGAGTGCTTGTCATGGAAAATGTCCTGTGGTGACAGGCCCAGTGGAACAGTCACCC TTCTGGGCAAGGGGGAACAAATCACACCTCTGGGCTTCAGGGTATCCCAGACCCCTCTCA ACACCCGCCCCCCCCATGTTTAAACTTTGTGCCTTTGACCATCTCTTAGGTCTAATGATA TTTTATGCAAACAGTTCTTGGACCCCTGAATTCAATGACAGGGATGCCAACACCTTCTTG GCTTCTGGGACCTGTGTTCTTGCTGAGCACCCTCTCCGGTTTGGGTTGGGATAACAGAGG CAGGAGTGGCAGCTGTCCCCTCTCCCTGGGGATATGCAACCCTTAGAGATTGCCCCAGAG CCCCACTCCCGGCCAGGCGGGAGATGGACCCCTCCCTTGCTCAGTGCCTCCTGGCCGGGG CCCCTCACCCCAAGGGGTCTGTATATACATTTCATAAGGCCTGCCCTCCCATGTTGCATG CCTATGTACTCTACGCCAAAGTGCAGCCCTTCCTCCTGAAGCCTCTGCCCTGCCTCCCTT TCTGGGAGGGCGGGGTGGGGGTGACTGAATTTGGGCCTCTTGTACAGTTAACTCTCCCAG GTGGATTTTGTGGAGGTGAGAAAAGGGGCATTGAGACTATAAAGCAGTAGACAATCCCCC ACATACCATCTGTAGAGTTGGAACTGCATTCTTTTAAAGTTTTATATGCATATATTTTAG GGCTGTAGACTTACTTTCCTATTTTCTTTTCCATTGCTTATTCTTGAGCACAAAATGATA ATCAATTATTACATTTATACATCACCTTTTTGACTTTTCCAAGCCCTTTTACAGCTCTTG GCATTTTCCTCGCCTAGGCCTGTGAGGTAACTGGGATCGCACCTTTTATACCAGAGACCT GAGGCAGATGAAATTTATTTCCATCTAGGACTAGAAAAACTTGGGTCTCTTACCGCGAGA CTGAGAGGCAGAAGTCAGCCCGAATGCCTGTCAGTTTCATGGAGGGGAAACGCAAAACCT GCAGTTCCTGAGTACCTTCTACAGGCCCGGCCCAGCCTAGGCCCGGGGTGGCCACACCAC AGCAAGCCGGCCCCCCCTCTTTTGGCCTTGTGGATAAGGGAGAGTTGACCGTTTTCATCC TGGCCTCCTTTTGCTGTTTGGATGTTTCCACGGGTCTCACTTATACCAAAGGGAAAACTC TTCATTAAAGTCCGTATTTCTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_003029 unedited
NNNCCATATAACACCCGCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCT ATATAAGCAGAGCTCATTTAGGTGACACTATGAGAATACACAGCTTACGTTGTTCTTTTT TGCAGNNCGGCCTGCGAATTCTGGCACTGAGGGCTGGGGAGAAAGTTGGAGCGGTAACCT AAGCTGGCAGTGGCGTGATCCGGCACCAAATCGGCCCGCGGTGCGGTGCGGAGACTCCAT GAGGCCCTGGACATGAACAAGCTGAGTGGAGGCGGCGGGCGCAGGACTCGGGTGGAAGGG GGCCAGCTTGGGGGCGAGGAGTGGACCCGCCACGGGAGCTTTGTCAATAAGCCCACGCGG GGCTGGCTGCATCCCAACGACAAAGTCATGGGACCCGGGGTTTCCTACTTGGTTCGGTAC ATGGGTTGTGTGGAGGTCCTCCAGTCAATGCGTGCCCTGGACTTCAACACCCGGACTCAG GTCACCAGGGAGGCCATCAGTCTGGTGTGTGAGGCTGTGCCGGGTGCTAAGGGGGCGACA AGGAGGAGAAAGCCCTGTAGCCGCCCGCTCAGCTCTATCCTGGGGAGGAGTAACCTGAAA TTTGCTGGAATGCCAATCACTCTCACCGTCTCCACCAGCAGCCTCAACCTCATGGCCGCA GACTGCAAACAGATCATCGCCAACCACCACATGCAATCTATCTCATTTGCATCCTGGCGG NGATCCGGACACAGCCTGAGTATGTCGCCTATGTTGCCAAAGACCCTGTGAATCAGAGAG CCTGCCACATTCTGGAGTGTCCCTGAAGGGCTTGCTCCAGGATGTCATCAGCACCATTGG CCAGGCCTTTCGAGTTGCGCTTCAACAGTACCTCAGGAACCACCCAGACTGGTACTCTCA TCACGNAC >OriGene 3' read for NM_003029 unedited
NGGGAATTACTATGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTT TAAAAAAAAACGGACTTTAATGAAGAGTTTTCCCTTTGGTATAAGTGAGACCCGTGGAAA CATCCAAACAGCAAAAGGAGGCCAGGATGAAAACGGTCAACTCTCCCTTATCCACAAGGC CAAAAGAGGGGGGGCCGGCTTGCTGTGGTGTGGCCACCCCGGGCCTAGGCTGGGCCGGGC CTGTAGAAGGTACTCAGGAACTGCAGGTTTTGCGTTTCCCCTCCATGAAACTGACAGGCA TTCGGGCTGACTTCTGCCTCTCAGTCTCGCGGTAAGAGACCCAAGTTTTTCTAGTCCTAG ATGGAAATAAATTTCATCTGCCTCAGGTCTCTGGTATAAAAGGTGCGATCCCAGTTACCT CACAGGCCTAGGCGAGGAAAATGCCAAGAGCTGTAAAAGGGCTTGGAAAAGTCAAAAAGG TGATGTATAAATGTAATAATTGATTATCATTTTGTGCTCAAGAATAAGCAATGGAAAAGA AAATAGGAAAGTAAGTCTACAGCCCTAAAATATATGCATATAAAACTTTAAAAGAATGCA GTTCCAACTCTACAGATGGTATGTGGGGGATTGTCTACTGCTTTATAGTCTCAATGCCCC CTTTCTCACCTCCACAAAATCCACCTGGGGAGAGTTAACTGTACAAGAAGCCCAAATTCA GTCACCCCCACCCCGCCCTCCCAAAAAGGGAGGCAGGGCAAAGGCCTTCAGGAGAAAAGG CTGCACCTTTGGCGTAAAGTACATAGGCATGCACCATGGGAGGGCAGCCCCTATGAAAAG TATTACCGACCCCTGGGGTGAGGGGCCCGGCCAGGAGCACTGAACAAGGAGGGGTCCATC TCCGCCTGGCCGGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003029 |
| Insert Size | 3200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to contain 3bp deletion compared with NM_003029.3. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003029.3, NP_003020.2 |
| RefSeq Size | 3076 bp |
| RefSeq ORF | 1425 bp |
| Locus ID | 6464 |
| UniProt ID | P29353 |
| Domains | SH2, PID |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Chemokine signaling pathway, Chronic myeloid leukemia, Dilated cardiomyopathy, ErbB signaling pathway, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis |
| Gene Summary | This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (2) has an alternate 5' sequence, as compared to variant 3. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 3. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Charting the molecular network of the drug target Bcr-Abl
,Marc Brehme, Oliver Hantschel, Jacques Colinge, Ines Kaupe, Melanie Planyavsky, Thomas Köcher, Karl Mechtler, Keiryn L. Bennett, and Giulio Superti-Furga,
PNAS, May 2009; 106: 7414 - 7419.
[SHC1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204362 | SHC1 (Myc-DDK-tagged)-Human SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2 |
CNY 3,656.00 |
|
| RC204362L1 | Lenti ORF clone of Human SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204362L2 | Lenti ORF clone of Human SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC204362L3 | Lenti ORF clone of Human SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204362L4 | Lenti ORF clone of Human SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG204362 | SHC1 (tGFP-tagged) - Human SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2 |
CNY 5,256.00 |
