CHKL (CHKB) (AK097775) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CHETK; CHKL; CK; CKB; CKEKB; EK; EKB; MDCMC
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK097775, the custom clone sequence may differ by one or more nucleotides GTCCGAAGGGAGCGGAGCGCAGCCTGGCCTGGGGCCCGGTCGAGCCCGCGCCATGGCGGCCGAGGCGACA GCTGTGGCCGGAAGCGGGGCTGTTGGCGGCTGCCTGGCCAAAGACGGCTTGCAGCAGTCTAAGTGCCCGG ACACTACCCCAAAACGGCGGCGCGCCTCGTCGCTGTCGCGTGACGCCGAGTGCAGCCCGAGGAGCTGAGG GTTTACCCCGTGAGCGGAGGCCTCAGCAACCTGCTCTTCCGCTGCTCGCTCCCGGACCACCTGCCCAGCG TTGGCGAGGAGCCCCGGGAGGTGCTTCTGCGGCTGTACGGAGCCATCTTGCAGGTGAGGGGGTTGTGAGC GCCGCAGCACCAGTGGCTTTAGGGCCTGTCGCTTACGCGATGCGGGTAGTATTGTTCCCGTTGCGCAGTT GAGGACACCGAGGTTCACGGTCTGAGTAACACCTCATTACACCGAAGCCTGGGCCTGTATTCCCAGAGCT TTGGGAGGCTGAGGCGAGAGGATCACTTGAGCACAGGAGTTCGAGACCAGCCTGGACAACATAGTGAGAC CCCCATCTCTAAATAAAAATAGACCAACGCTAAAGCCTGTGCTCCAGAGCCTCCAGGCAATTGGATCAGA AGTCGCAGCTCTGGTGGGAGGAAGGCGAGCCCTCATGTGTGTCCCTGTGCCACTTTGCCTTGGCCCCTTT GCTGTCCATCCTTTTTCAGGGCGTGGACTCCCTGGTGCTAGAAAGCGTGATGTTCGCCATACTTGCGGAG CGGTCGCTGGGGCCCCAGCTGTACGGAGTCTTCCCAGAGGGCCGGCTGGAACAGTACATCCCAGTACGGG CCCAGTCCTACCCTCTCCTCCCCAAGGCACCTCCACCCCCTAACCCTACCCCAGTGCCCAATGTCTGCCT CCACATCCCTCACCCCAAGTAGGGAATTTCCCCCAAAACCCTGACTTCCCCTCATTGCCCCAGCCCTTCC CACTCCTGCCCCAGCCCCATCACCACCCTGATAGCTTCCTGGGTGCAGAGTCGGCCATTGAAAACTCAAG AGCTTCGAGAGCCAGTGTTGTCAGCAGCCATTGCCACGAAGATGGCGCAATTTCATGGCATGGAGATGCC TTTCACCAAGGAGCCCCACTGGCTGTTTGGGACCATGGAGCGGTACCTAAAACAGATCCAGGACCTGCCC CCAACTGGCCTCCCTGAGATGAACCTGCTGGAGATGTACAGCCTGAAGGATGAGATGGGCAACCTCAGGA AGTTACTAGAGTCTACCCCATCGCCAGTCGTCTTCTGCCACAATGACATCCAGGAAGGGAACATCTTGCT GCTCTCAGAGCCAGAAAATGCTGACAGCCTCATGCTGGTGGACTTCGAGTACAGCAGTTATAACTATAGG GGCTTTGACATTGGGAACCATTTTTGTGAGTGGGTTTATGATTATACTCACGAGGAATGGCCTTTCTACA AAGCAAGGCCCACAGACTACCCCACTCAAGAACAGCAGAGGCAAAGAAAGGTGAGACCCTCTCCCAAGAG GAGCAGAGAAAACTGGAAGAAGATTTGCTGGTAGAAGTCAGTCGGTATGCTCTGGCATCCCATTTCTTCT GGGGTCTGTGGTCCATCCTCCAGGCATCCATGTCCACCATAGAATTTGGTTACTTGGACTATGCCCAGTC TCGGTTCCAGTTCTACTTCCAGCAGAAGGGGCAGCTGACCAGTGTCCACTCCTCATCCTGACTCCACCCT CCCACTCCTTGGATTTCTCCTGGAGCCTCCAGGGCAGGACCTTGGAGGGAGGAACAACGAGCAGAAGGCC CTGGCGACTGGGCTGAGCCCCCAAGTGAAACTGAGGTTCAGGAGACCGGCCTGTTCCTGAGTTTGAGTAG GTCCCCATGGCTGGCAGGCCAGAGCCCCGTGCTGTGTATGTAACACAATAAACAAGCTTCTTCTTCC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK097775 unedited NCGGTTCAAAATTGTATACGACTCCTATAGGCGGCCGCGNATTCGGCACGAGGCGGAGCG CAGCCTGGCCTGGGGCCCGGTCGAGCCCGCGCCATGGCGGCCGAGGCGACAGCTGTGGCC GGAAGCGGGGCTGTTGGCGGCTGCCTGGCCAAAGACGGCTTGCAGCAGTCTAAGTGCCCG GACACTACCCCAAAACGGCGGCGCGCCTCGTCGCTGTCGCGTGACGCCGAGCGCCGAGCC TACCAATGGTGCCGGGAGTACTTGGGCGGGGCCTGGCGCCGAGTGCAGCCCGAGGAGCTG AGGGTTTACCCCGTGAGCGGAGGCCTCAGCAACCTGCTCTTCCGCTGCTCGCTCCCGGAC CACCTGCCCAGCGTTGGCGAGGAGCCCCGGGAGGTGCTTCTGCGGCTGTACGGAGCCATC TTGCAGGGCGTGGACTCCCTGGTGCTAGAAAGCGTGATGTTCGCCATACTTGCGGAGCGG TCGCTGGGGCCCCAGCTGTACGGAGTCTTCCCAGAGGGCCGGCTGGAACAGTACATCCCA AGTCGGCCATTGAAAACTCAAGAGCTTCGAGAGCCAGTGTTGTCAGCAGCCATTGCCACG AAGATGGCGCAATTTCATGGCATGGAGATGCCTTTCACCAAGGAGCCCCACTGGCTGTTT GGGACCATGGAGCGGTACCTAAAACAGATCCAGGACCTGCCCCCAACTGGCCTCCCTGAG ATGAACCTGCTGGAGATGTACAGCCTGAAGGATGAGATGGGCAACCTCANGAAGTTACTA GAGTCTACCCATCGNCAGTCGTCTTCTGCCACATGACACCAGGAAGGTANGAGAAGCATC TGAGTCTCCTAACCCAGATGGAGAGCCAGAGGCTCTGGAGTGAGCAGAACTCACCCATTC CCCAGGNACATCTGCTGCTCTCAGAGCCAGAATGCTGACAGCTCATGCTGTGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK097775 unedited NNNAACGTTAAGCTATGNACCGCGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTT TTTGCGAAAAAAAACTTGTTTATTGTGTTACATACACAGCACGGGGCTCTGGCCTGCCAG CCATGGGGACCTACTCAAACTCAGGGACAGGCCGGTCTCCTGAACCTCAGTTTCACTTGG GGGCTCAGCCCAGTCGCCAGGGCCTTCTGCTCGTTGTTCCTCCCTCCAAGGTCCTGCCCT GGAGGCTCCAGGAGAAATCCAAGGAGTGGGAGGGTGGAGTCAGGATGAGGAGTGGACACT GGTCAGCTGCCCCTTCTGCTGGAAGTAGAACTGGAACCGAGACTGGGCATAGTCCAAGTA ACCAAATTCTATGGTGGACATGGATGCCTGGAGGATGGACCACAGACCCCAGAAGAAATG GGATGCCAGAGCATACCGACTGACTTCTACCAGCAAATCTTCTTCCAGTTTTCTCTGCTC CTCTTGGGAGAGGGTCTCACCTTTCTTTGCCTCTGCCAGGTAATGACGAATAAAATGCAA CTGCTGTTCTTGAGTGGGGTAGTCTGTGGGCCTTGCTTTGTAGAAAGGCCATTCCTCGTG AGTATAATCATAAACCCACTCACAAAAATGGTTCCCAATGTCAAAGCCCCTATAGTTATA ACTGCTGTACTCGAAGTCCACCAGCATGAGGCTGTCAGCATTTTCTGGCTCTGAGAGCAG CAAGATGTTCCCTGGGGGAATGGGGGTGAGGTTCTGCTCACTCCAGAGCCCTCTGGCTCT TCCATCTTGGGTTAAGAGACTCAGATGCCTTCTNCTACCTTCCCGGATGTCATTGTGGCA GAAGACGACTGGCGATGGGGTG
Restriction Sites	NotI-NotI
ACCN	AK097775
Insert Size	1650 bp
OTI Disclaimer	The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required.
OTI Annotation	This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK097775.1
RefSeq Size	1957 bp
RefSeq ORF	1957 bp
Locus ID	1120
Domains	Choline_kinase
Protein Families	Druggable Genome
Protein Pathways	Glycerophospholipid metabolism, Metabolic pathways
Gene Summary	Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]

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