AP2S1 (BC006337) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	adaptor-related protein complex 2, sigma 1 subunit; AP17; AP17-DELTA; CLAPS2; clathrin-associated/assembly/adaptor protein, small 2 (17kD); clathrin assembly protein 2 small chain
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC006337 edited GCGGGACCGGGGCCGCTGGGGTCTGGACGGGGGTCGCCATGATCCGCTTTATCCTCATCC AGAACCGGGCAGGCAAGACGCGCCTGGCCAAGTGGTACATGCAGTTTGATGATGATGAGA AACAGAAGCTGATCGAGGAGGTGCATGCCGTGGTCACCGTCCGAGACGCCAAACACACCA ACTTTGTGGAGTTCCGGAACTTTAAGATCATTTACCGCCGCTATGCTGGCCTCTACTTCT GCATCTGTGTGGATGTCAATGACAACAACCTGGCTTACCTGGAGGCCATTCACAACTTCG TGGAGGTCTTAAACGAATATTTCCACAATGTCTGTGAACTGGACCTGGTGTTCAACTTCT ACAAGGTTTACACGGTCGTGGACGAGATGTTCCTGGCTGGCGAAATCCGAGAGACCAGCC AGACGAAGGTGCTGAAACAGCTGCTGATGCTACAGTCCCTGGAGTGAGGGCAGGCGAGCC CCACCCCGGCCCCGGCCCCTCCTGGACTCGCCTGCTCGCTTCCCCTTCCCAGGCCCGTGG CCAACCCAGCAGTCCTTCCCTCAGCTGCCTAGGAGGAAGGGACCCAGCTGGGTCTGGGCC ACAAGGGAGGAGACTGCACCCCACTGCCTCTGGGCCCTGGCTGTGGGCAGAGGCCACCGT GTGTGTCCCGAGTAACCGTGCCGTTGTCGTGTGATGCCATAAGCGTCTGTGCGTGGAGTC CCCAATAAACCTGTGGTCCTGCCTGGAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC006337 unedited GTTACGATTTTGTATACGACTTATATAGGGCGGCCGCGAATTCGCACGAGGGCGGGACCG GGGCCGCTGGGGTCTGGACGGGGGTCGCCATGATCCGCTTTATCCTCATCCAGAACCGGG CAGGCAAGACGCGCCTGGCCAAGTGGTACATGCAGTTTGATGATGATGAGAAACAGAAGC TGATCGAGGAGGTGCATGCCGTGGTCACCGTCCGAGACGCCAAACACACCAACTTTGTGG AGTTCCGGAACTTTAAGATCATTTACCGCCGCTATGCTGGCCTCTACTTCTGCATCTGTG TGGATGTCAATGACAACAACCTGGCTTACCTGGAGGCCATTCACAACTTCGTGGAGGTCT TAAACGAATATTTCCACAATGTCTGTGAACTGGACCTGGTGTTCAACTTCTACAAGGTTT ACACGGTCGTGGACGAGATGTTCCTGGCTGGCGAAATCCGAGAGACCAGCCAGACGAAGG TGCTGAAACAGCTGCTGATGCTACANTCCCTGGAATGAGGGCAGGCGAGCCCCACCCCGG CCCCGGCCCCTCCTGGACTCGCCTGCTCGCTTCCCCTTCCCAGGCCCGTGGCCAACCCAG CAGTCCTTCCCTCAGCTGCCTAGGAGGAAGGGACCCAGCTGGGTCTGGGCCACAAGGGAG GAGACTGCACCCCACTGCCTCTGGGCCCTGGCTGTGGGCAGAAGCCACCGTGTGTGTCCC GAGTAACCGTGCCGTTGTCGTGTGATGCCATAAGCGTCTGTGCGTGGAGTCCCCATAAAC CTGTGGTCCTGCCTGGAAAAAAAAAAAAAAAACTGAGGTTNTTTTNNNNNGCGCGGGGGG TGNGTTGCTGGGCGCCCGCTGGGNTTTTTTTTTTTTTTTCCCAGGGGGGACTTTTTATGC GGAGAGAGAGGGGGTGTCTTCTTTTTGGGGGGCGGG
Restriction Sites	Please inquire
ACCN	BC006337
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC006337.2, AAH06337.1
RefSeq Size	764 bp
Locus ID	1175
Protein Pathways	Endocytosis, Huntington's disease
Gene Summary	One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

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