GJA4 (BC027889) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	connexin 37; CX37; gap junction protein, alpha 4, 37kDa; OTTHUMP00000004181; OTTHUMP00000046201
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC027889 edited GCGGGCGTCACTCCGGCCATCGTCCCCACCTCCACCTGGGCCGCCCGGCAGGCAGGCGAC GGAGGCCCGGGAGCCATGGGTGACTGGGGCTTCCTGGAGAAGTTGCTGGACCAGGTCCAG GAGCACTCGACCGTGGTGGGTAAGATCTGGCTGACGGTGCTCTTCATCTTCCGCATCCTC ATCCTGGGCCTGGCCGGCGAGTCAGTGTGGGGTGACGAGCAGTCAGATTTCGAGTGTAAC ACGGCCCAGCCAGGCTGCACCAACGTCTGCTATGACCAGGCCTTCCCCATCTCCCACATC CGCTACTGGGTGCTGCAGTTCCTCTTCGTCAGCACACCCACCCTGGTCTACCTGGGCCAT GTCATTTACCTGTCTCGGCGAGAAGAGCGGCTGCGGCAGAAGGAGGGGGAGCTGCGGGCA CTGCCGGCCAAGGACCCACAGGTGGAGCGGGCGCTGGCGGCCGTAGAGCGTCAGATGGCC AAGATCTCGGTGGCAGAAGATGGTCGCCTGCGCATCCGCGGAGCACTGATGGGCACCTAT GTCGCCAGTGTGCTCTGCAAGAGTGTGCTAGAGGCAGGCTTCCTCTATGGCCAGTGGCGC CTGTACGGCTGGACCATGGAGCCCGTGTTTGTGTGCCAGCGAGCACCCTGCCCCTACCTC GTGGACTGCTTTGTCTCTCGCCCCACGGAGAAGACCATCTTCATCATCTTCATGTTGGTG GTTGGACTCATCTCCCTGGTGCTTAACCTGCTGGAGTTGGTGCACCTGCTGTGTCGCTGC CTCAGCCGGGGGATGAGGGCACGGCAAGGCCAAGACGCACCCCCGACCCAGGGCACCTCC TCAGACCCTTACACGGACCAGGTCTTCTTCTACCTCCCCGTGGGCCAGGGGCCCTCATCC CCACCATGCCCCACCTACAATGGGCTCTCATCCAGTGAGCAGAACTGGGCCAACCTGACC ACAGAGGAGAGGCTGGCGTCTTCCAGGCCCCCTCTCTTCCTGGACCCACCCCCTCAGAAT GGCCAAAAACCCCCAAGTCGTCCCAGCAGCTCTGCTTCTAAGAAGCAGTATGTATAGAGG CCTGTGGCTTATGTCACCCAACAGAGGGGTCCTGAGAAGTCTGGCTGCCTGGGATGCCCC CTGCCCCCTCCTGGAAGGCTCTGCAGAGATGACTGGGCTGGGGAAGCAGATGCTTGCTGG CCATGGAGCCTCATTGCAAGTTGTTCTTGAACACCTGAGGCCTTCCTGGTGCCCACCAGG CACTACGGCTTCCTCTCCAGAATGTGGCTTTGCCTGAGCACAGACAGTCAGCATGGAATG CTCTTGGCCAAGGGTACTGGGGGCCCTCTGGCCTTTTGCAGCTGATCCAGAGGAACCCAG AGCCAACTTACCCCAACCTCACCCTATGGAACAGTCACCTGTGCGCAGGTTGTCCTCAAA CCCTCTCCTCACAGGAAAAGGCGGATTGAGGCTGCTGGGTCAGCCTTGATGGCACAGACA GAGCTTGTGCCGGATTTGGCCCTGTCAAGGGGACTGGTGCCTTGTTTTCATCACTCCTTC CTAGTTCTACTGTTCAAGCTTCTGAAATAAACAGGACTTGATCACAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC027889 unedited GTTCTGATTTGTATACCATTATATAGGCGGCCGCGNAATTCAATCTGGTACCGGTCCGGA ATTCCCGGGATGCGGGCGTCACTCCGGCCATCGTCCCCACCTCCACCTGGGCCGCCCGGC AGGCAGGCGACGGAGGCCCGGGAGCCATGGGTGACTGGGGCTTCCTGGAGAAGTTGCTGG ACCAGGTCCAGGAGCACTCGACCGTGGTGGGTAAGATCTGGCTGACGGTGCTCTTCATCT TCCGCATCCTCATCCTGGGCCTGGCCGGCGAGTCAGTGTGGGGTGACGAGCAGTCAGATT TCGAGTGTAACACGGCCCAGCCAGGCTGCACCAACGTCTGCTATGACCAGGCCTTCCCCA TCTCCCACATCCGCTACTGGGTGCTGCAGTTCCTCTTCGTCAGCACACCCACCCTGGTCT ACCTGGGCCATGTCATTTACCTGTCTCGGCGAGAAGAGCGGCTGCGGCAGAAGGAGGGGG AGCTGCGGGCACTGCCGGCCAAGGACCCACAGGTGGAGCGGGCGCTGGCGGCCGTAGAGC GTCAGATGGCCAAGATCTCGGTGGCAGAAGATGGTCGCCTGCGCATCCGCGGAGCACTGA TGGGCACCTATGTCGCCAGTGTGCTCTGCAAGAGTGTGCTAGAGGCAGGCTTCCTCTATG GCCAGTGGCGCCTGTACNGCTGGACCATGGAGCCCGTGTTTGTGTGCCAGCGAGCACCCT GCCCCTACCTCGTGGACTGGCTTGTCTCTCGCCCCACGGAGAAGACCATCTCATCATCTT CATGTTGGTGGGTGGNACTCATCTNCCTGGTGCCTAACCTGCTGGAGTTGGNTGCACCTG CTGTGTCGCTGCCCTCACCGGGGGATGAAGGGCACGGCAAGCCCAGAACGCACCCCCGAC CAAGGCACCTC
Restriction Sites	NotI-NotI
ACCN	BC027889
Insert Size	1600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027889.1, AAH27889.1
RefSeq Size	1647 bp
RefSeq ORF	999 bp
Locus ID	2701
Protein Families	Ion Channels: Other, Transmembrane
Gene Summary	This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. [provided by RefSeq, Jul 2008]

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