SNRNP25 (BC009179) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C16orf33; FLJ22940; minus -99 protein; small nuclear ribonucleoprotein 25kDa (U11/U12); U11/U12 snRNP 25K protein
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009179 edited GGCACGAGGCCGGCTGAGAGGGGCGGCCCTGGAGGAGACGGAGGCGGCGGGTGGGCCCGA GGCGCAAGAGGAAGATGAGGACGAAGAAGAGGCGCTGCCGCACTCCGAGGCCATGGACGT GTTCCAGGAGGGTCTGGCTATGGTGGTGCAGGACCCGCTGCTCTGCGATCTGCCGATCCA GGTTACTCTGGAAGAAGTCAACTCCCAAATAGCCCTAGAATACGGCCAGGCAATGACGGT CCGAGTGTGCAAGATGGATGGAGAAGTAATGCCCGTGGTTGTAGTGCAGAGTGCCACAGT CCTGGACCTGAAGAAGGCCATCCAGAGATACGTGCAGCTCAAGCAGGAGCGTGAAGGGGG CATTCAGCACATCAGCTGGTCCTACGTGTGGAGGACGTACCATCTGACCTCTGCAGGAGA GAAACTCACGGAAGACAGAAAGAAGCTCCGAGACTACGGCATCCGGAATCGAGACGAGGT TTCCTTCATCAAAAAGCTGAGGCAAAAGTGAGCCTCCAGACAGGACAACCCTCTTCATCA CTGGTGGCTGAGCTTTTTCCCAGCAGGAATGGGTCCTCGAATCATCGTGCCTCTTTCACA GAAAGGACGTTGTGGTGGCCTCACCCCAGGCATGCCCAACAGGAACTGTCAGCATAAACC TGGGGGCCCTCAGGACTAGGACAGGGTGAGCCAGTGCTCCCTCCTTTCATGTACTTGGCC TGAGACTGACCTCTCCCTAGGTCCAAATGCCCTAGTCACATGGCAGACCCACGGCCTGGC CCACTGTATAAAATAAACCTGTTTGCTTCTTAGTTTGAAAAAAAAAAAAAAAAAAAAAAA AA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009179 unedited NGGGGAGTCGGATTTTGTATACGACTCATATAGGCGGACCGCGAATTCGCACGAGGCCGG CTGAGAGGGGCGGCCCTGNGAGAGACGGAGGCGGCGGGTGGGCCCGAGGCGCAAGAGGAA GATGAGGACGAAGAAGAGGCGCTGCCGCACTCCGAGGCCATGGACGTGTTCCAGGAGGGT CTGGCTATGGTGGTGCAGGACCCGCTGCTCTGCGATCTGCCGATCCAGGTTACTCTGGAA GAAGTCAACTCCCAAATAGCCCTAGAATACGGCCAGGCAATGACGGTCCGAGTGTGCAAG ATGGATGGAGAAGTAATGCCCGTGGTTGTAGTGCAGAGTGCCACAGTCCTGGACCTGAAG AAGGCCATCCAGAGATACGTGCAGCTCAAGCAGGAGCGTGAAGGGGGCATTCAGCACATC ACCTGGGTCTACCTGTGGAGGACGTACCATTTGACCTCTTCGGGAAAAAACCTCCCGGAA AACCAAAAGAAACCTCCAACATACCGGATCCGGAAATTGAAAAAGGGTTTCCTTTTTTAA AAACTTGGGGAAAATGGGCGCCCCCAAAGGAGACCCCCCTTTTAAAAGGGGGGGGGGAGT TTTTTCCCCCAGGAGGGGGGGCCCAAAAAAAAGCGCCCCTTTCAAAAAAAAAGAGAGGTT GGGGGGGTGTCCCCCCCCCGGTGCCCAAAAGAGAAGTTTTTATATAAATATTGGCGGGCC CCCCAACAAAAAGAAAAGAAGGGGGAGAACCCCCCCCCCTCCTTTTTTGTTTTGGGGGGG GGAGGGACCCTCCCCCCCCCAAAAGGGCCCCACNAAAGGAGAGGCCGCCACCCCGCCGGC CCCCCCCCAAAAAAAAAAAAAAATTTTTTTTTTTTTTTTTTATAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAGCCGGCGCCGGGGGCC
Restriction Sites	Please inquire
ACCN	BC009179
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009179.1, AAH09179.1
RefSeq Size	842 bp
Locus ID	79622
Protein Families	Druggable Genome
Gene Summary	Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. This gene encodes a 25K protein that is a component of the U12-type spliceosome. [provided by RefSeq, Apr 2010]

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