Glycerol kinase (GK) (BC037549) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATP:glycerol 3-phosphotransferase; GK1; GKD; glycerokinase; glycerol kinase
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC037549 edited CCGGCCGCAATCGCCGGCCGACCTGAAGCTGGTTTCATGGCAGCCTCAAAGAAGGCAGTT TTGGGGCCATTGGTGGGGGCGGTGGACCAGGGCACCAGTTCGACGCGCTTTTTGGTTTTC AATTCAAAAACAGCTGAACTACTTAGTCATCATCAAGTAGAAATAAAACAAGAGTTCCCA AGAGAAGGATGGGTGGAACAGGACCCTAAGGAAATTCTACATTCTGTCTATGAGTGTATA GAGAAAACATGTGAGAAACTTGGACAGCTCAATATTGATATTTCCAACATAAAAGCTATT GGTGTCAGCAACCAGAGGGAAACCACTGTAGTCTGGGACAAGATAACTGGAGAGCCTCTC TACAATGCTGTGGTGTGGCTTGATCTAAGAACCCAGTCTACCGTTGAGAGTCTTAGTAAA AGAATTCCAGGAAATAATAACTTTGTCAAGTCCAAGACAGGCCTTCCACTTAGCACTTAC TTCAGTGCAGTGAAACTTCGTTGGCTCCTTGACAATGTGAGAAAAGTTCAAAAGGCCGTT GAAGAAAAACGAGCTCTTTTTGGGACTATTGATTCATGGCTTATTTGGAGTTTGACAGGA GGAGTCAATGGAGGTGTCCACTGTACAGATGTAACAAATGCAAGTAGGACTATGCTTTTC AACATTCATTCTTTGGAATGGGATAAACAACTCTGCGAATTTTTTGGAATTCCAATGGAA ATTCTTCCAAATGTCCGGAGTTCTTCTGAGATCTATGGCCTAATGAAAGCTGGGGCCTTG GAAGGTGTGCCAATATCTGGGTGTTTAGGGGACCAGTCTGCTGCATTGGTGGGACAAATG TGCTTCCAGATTGGACAAGCCAAAAATACGTATGGAACAGGATGTTTCTTACTATGTAAT ACAGGCCATAAGTGTGTATTTTCTGATCATGGCCTTCTCACCACAGTGGCTTACAAACTT GGCAGAGACAAACCAGTATATTATGCTTTGGAAGGTTCTGTAGCTATAGCTGGTGCTGTT ATTCGCTGGCTAAGAGACAATCTTGGAATTATAAAGACCTCAGAAGAAATTGAAAAACTT GCTAAAGAAGTAGGTACTTCTTATGGCTGCTACTTCGTCCCAGCATTTTCGGGGTTATAT GCACCTTATTGGGAGCCCAGCGCAAGAGGGATAATCTGTGGACTCACTCAGTTCACCAAT AAATGCCATATTGCTTTTGCTGCATTAGAAGCTGTTTGTTTCCAAACTCGAGAGATTTTG GATGCCATGAATCGAGACTGTGGAATTCCACTCAGTCATTTGCAGGTAGATGGAGGAATG ACCAGCAACAAAATTCTTATGCAGCTACAAGCAGACATTCTGTATATACCAGTAGTGAAG CCCTCAATGCCCGAAACCACTGCACTGGGTGCGGCTATGGCGGCAGGGGCTGCAGAAGGA GTCGGCGTATGGAGTCTCGAACCCGAGGATTTGTCTGCCGTCACGATGGAGCGGTTTGAA CCTCAGATTAATGCGGAGGAAAGTGAAATTCGTTATTCTACATGGAAGAAAGCTGTGATG AAGTCAATGGGTTGGGTTACAACTCAATCTCCAGAAAGTGGTATTCCATAAAACCTACCA ACTCATGGATTCCCAAGATGTGAGCTTTTTACATAATGAAAGAACCCAGCAATTCTGTCT CTTAATGCAATGACACTATTCATAGACTTTGATTTTATTTATAAGCCACTTGCTGCATGA CCCTCCAAGTAGACCTGTGGCTTAAAATAAAGAAAATGCAGCAAAAAGAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC037549 unedited TCTGATTTTGTATACGACTCTATATAGGCGGCCGCAGATTCAGATCTGGTACCGGTCCGG AATTCCCGGGATCCGGCCGCAATCGCCGGCCGACCTGAAGCTGGTTTCATGGCAGCCTCA AAGAAGGCAGTTTTGGGGCCATTGGTGGGGGCGGTGGACCAGGGCACCAGTTCGACGCGC TTTTTGGTTTTCAATTCAAAAACAGCTGAACTACTTAGTCATCATCAAGTAGAAATAAAA CAAGAGTTCCCAAGAGAAGGATGGGTGGAACAGGACCCTAAGGAAATTCTACATTCTGTC TATGAGTGTATAGAGAAAACATGTGAGAAACTTGGACAGCTCAATATTGATATTTCCAAC ATAAAAGCTATTGGTGTCAGCAACCAGAGGGAAACCACTGTAGTCTGGGACAAGATAACT GGAGAGCCTCTCTACAATGCTGTGGTGTGGCTTGATCTAAGAACCCAGTCTACCGTTGAG AGTCTTAGTAAAAGAATTCCAGGAAATAATAACTTTGTCAAGTCCAAGACAGGCCTTCCA CTTAGCACTTACTTCAGTGCAGTGAAACTTCGTTGGCTCCTTGACAATGTGAGAAAAGTT CNAAGGCCGTTGAAGAAAAACGAGCTCTTTTGGGACTATTGATTCATGGCTTATTTTGAG TTTGACAGGAGGAGTCAATGGAAGTGTCCACTGTACAGATGTAACAAATGGCAGTANGAC TATGCTTTTCAACATTCATTCTTTGGAATGGGATAAAACACTCTGCGGATTTTTTGGAAT TCCAATGGNAAATCCTTCCAATGTCCGGAGTTCTTCTGAGATCTATGGCCTAATGAAAAC TGGGGCTTTGNAAGGGTGCCATTTCTTGGTGGTTAAGGGACCAGTCTGCTGCTTGGTGGG ACAAATTTGCTTTCCAATGGGCCAAGCAAAATACCTTTGGGACAGG
Restriction Sites	Please inquire
ACCN	BC037549
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC037549.1, AAH37549.1
RefSeq Size	1835 bp
Locus ID	2710
Protein Families	Druggable Genome
Protein Pathways	Glycerolipid metabolism, Metabolic pathways, PPAR signaling pathway
Gene Summary	The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

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