ZNF207 (BC008023) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZp761N202; OTTHUMP00000163700; zinc finger protein 207
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC008023 edited GCTTCCTGTGGGACGTGGTGGTAGCCGTTGGGTTGGGAAAGTGAGGGATTTTTGGCCTCG TTTCTCCTGCTTCTTTTCTCCTCCCTTTTACTTTGCCGGTAGAACACAGTTATGGGTCGC AAGAAGAAGAAGCAGCTGAAGCCGTGGTGCTGGTATTGTAATAGAGATTTTGATGATGAG AAGATCCTTATTCAGCACCAAAAAGCAAAGCATTTTAAATGCCATATATGTCACAAGAAA TTGTATACAGGACTGCCACCAGTACCAGGAGCACCAGGAATGCCTCCAGGCATACCTCCA TTAATGCCAGGTGTTCCTCCTCTGATGCCAGGAATGCCACCAGTTATGCCAGGCATGCCA CCTGGATTGCATCATCAGAGAAAATACACCCAGTCATTTTGCGGTGAAAACATAATGATG CCAATGGGTGGAATGATGCCACCTGGACCAGGAATACCACCTCTGATGCCTGGAATGCCA CCAGGTATGCCCCCACCTGTTCCACGTCCTGGAATTCCTCCAATGACTCAAGCACAGGCT GTTTCAGCGCCAGGTATTCTTAATAGACCACCTGCACCAACAGCAACTGTACCTGCCCCA CAGCCTCCAGTTACTAAGCCTCTTTTCCCCAGTGCTGGACAGATGGGGACACCTGTCACA AGCTCAAGTACAGCTTCATCCAATTCAGAAAGTCTGTCTGCATCTTCTAAAGCTCTGTTT CCTAGCACAGCACAAGCTCAGGCAGCTGTCCAAGGACCTGTTGGTACAGATTTCAAACCC TTAAATAGTACCCCTGCAACAACTACAGAACCCCCAAAGCCTACATTCCCTGCTTATACA CAGTCTACAGCTTCAACAACTAGTACAACAAATAGTACTGCAGCTAAACCAGCGGCTTCA ATAACAAGTAAGCCTGCTACACTTACAACAACTAGTGCAACCAGTAAGTTGATCCATCCA GATGAGGATATATCCCTGGAAGAGAGAAGGGCACAGTTACCTAAGTATCAACGTAATCTT CCTCGGCCAGGACAGGCCCCCATCGGTAATCCACCAGTTGGACCAATTGGAGGTATGATG CCACCACAGCCAGGCATCCCACAGCAACAAGGAATGAGACCCCCAATGCCACCTCATGGT CAGTATGGTGGTCATCATCAAGGCATGCCAGGATACCTTCCTGGTGCTATGCCCCCGTAT GGGCAGGGACCGCCAATGGTGCCCCCTTACCAGGGTGGGCCTCCTCGACCTCCGATGGGA ATGAGACCTCCTGTAATGTCGCAAGGTGGCCGTTACTGATCTTACTTCATCCAGTCTAAT AGGTTTGGAGATTAAACCTTTTCTCAACTTGTGCTGTTTATATAGCCAAGCTTCCGTCAA TAAGGCTTCATTGTGACTTTAACAAACATTATCTTCCCACATACCAGGAACTATTGGACA TTTATTTTACATGGGAAAAATTATTTGGAATAATAAAGCAGGAACTTTTCCTGAAGTTGC AATTTATACTGTATGGCTTCTTTTTCATGTTTCATCTAGGTTTTTAGAAGTGAAGTATAG TAAATTTGGTTCGTTAAATTGTGAAGGCGCTGGAATTACATGAACATACCACCCTAGTAA AGGCAAGTTCTGTAAGCTTACATTGCTATTTGTAAAGTTTGCCTTCACAGCATTTCAGAT GCTGTTGGACTTCATGTCCCCAACCTAGCTTGGTGAGGGCTGTAACTGTTTCCAAGTACT TGTACATTGGAAGTCTGAATGTGTAACAATATTTAATGTATTTAGAGTTCCTCATGTTGC AGGGTTTAAGAAATCTGACCCACCAAGGTCATGTGACTTTTCTGTACTGTTAAACTTCAT TGTAATAAAATGAGAGAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008023 unedited NCCCCGTTCAAGATTTTGTNAATACGNATNCACNATAGGGNCGGACCGCGNAATTCGGCA CGAGGGCTTCCTGTGGGACGTGGTGGTAGCCGTTGGGTTGGGAAAGTGAGGGATTTTTGG CCTCGTTTCTCCTGCTTCTTTTCTCCTCCCTTTTACTTTGCCGGTAGAACACAGTTATGG GTCGCAAGAAGAAGAAGCAGCTGAAGCCGTGGTGCTGGTATTGTAATAGAGATTTTGATG ATGAGAAGATCCTTATTCAGCACCAAAAAGCAAAGCATTTTAAATGCCATATATGTCACA AGAAATTGTATACAGGACTGCCACCAGTACCAGGAGCACCAGGAATGCCTCCAGGCATAC CTCCATTAATGCCAGGTGTTCCTCCTCTGATGCCAGGAATGCCACCAGTTATGCCAGGCA TGCCACCTGGATTGCATCATCAGAGAAAATACACCCAGTCATTTTGCGGTGAAAACATAA TGATGCCAATGGGTGGAATGATGCCACCTGGACCAGGAATACCACCTCTGATGCCTGGAA TGCCACCAGGTATGCCCCCACCTGTTCCACGTCCTGGAATTCCTCCAATGACTCAAGCAC AGGCTGTTTCAGCGCCAGGTATTCTTAATAGACCACCTGCACCAACAGCAACTGTACCTG CCCCACAGCCTCCAGTTACTAAGCCTCTTTTCCCCAGTGCTGGACAGATGGGGACACCTG TCACAAGCTCAAGTACAGCTTCATCCAATTCAGAAAGTCTGTCTGCATCTTCTAAAGCTC TGTTTCCTAGCACAGCACAAGCTCAGGCAGCTGTCCAAGGACCTGTTGGTACAGATTTCA AAACCCTAAATAGTACCCCTGGCACAACTACAGAAACCCCAAGGCTACATTCCCTGGCTA TACACAGTCTACAGCTTCAAA
Restriction Sites	Please inquire
ACCN	BC008023
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008023.1, AAH08023.1
RefSeq Size	1897 bp
Locus ID	7756
Protein Families	Transcription Factors
Gene Summary	Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed:24462186, PubMed:24462187, PubMed:26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed:24462186, PubMed:24462187). Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).[UniProtKB/Swiss-Prot Function]

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