Aldolase (ALDOA) (BC010568) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC010568 edited CAGCTGAATAGGCTGCGTTCTCTTGGAACGCGCAGCAGAACGAGGTTCTGGTGACCCTAG CCGCGTTCGCTCCTTAGTCCTTTCGCCTACCCACCGGCGTACCAGGCAGACCCACCCCGT CCTGTGCCAGGAAAGCACTGCCACCGGCACCATGCCCCACCCATACCCAGCACTGACCCC GGAGCAGAAGAAGGAGCTGTCTGACATCGCTCACCGCATTGTGGCTCCGGGCAAGGGCAT CCTGGCTGCAGATGAGTCCACCGGAAGCATTGCCAAGCGCCTGCAGTCCATTGGCACCGA GAACACCGAGGAGAACAGGCGCTTCTACCGCCAGCTGCTGCTGACTGCAGACGACCGTGT GAATCCCTGCATTGGGGGGGTGATCCTCTTCCACGAGACACTGTACCAGAAGGCAGATGA TGGACGTCCCTTCCCCCAAGTTATCAAGTCCAAGGGTGGTGTTGTGGGCATTAAGGTAGA TAAGGGTGTGGTGCCCCTGGCAGGAACCAATGGCGAGACAACTACCCAGGGGCTGGATGG GCTGTCTGAACGCTGTGCCCAGTATAAGAAGGATGGAGCCGACTTTGCCAAGTGGCGCTG TGTGCTAAAGATTGGGGAACATACTCCCTCGGCCCTGGCCATCATGGAAAATGCCAATGT TCTGGCCCGTTATGCCAGCATCTGCCAGCAGAATGGCATTGTACCCATTGTGGAGCCTGA AATTCTCCCTGATGGGGACCATGACTTGAAGCGCTGCCAGTATGTTACTGAGAAGGTCCT GGCGGCTGTCTACAAGGCTCTGAGCGACCACCATGTCTATCTGGAAGGCACATTGCTGAA GCCCAACATGGTCACCCCTGGCCATGCTTGCACCCAGAAATTTTCCAATGAGGAGATTGC CATGGCAACGGTCACAGCACTTCGTCGCACAGTGCCCCCTGCTGTCACTGGGGTCACTTT CCTGTCTGGAGGGCAGAGTGAGGAAGAGGCATCCATCAACCTCAATGCTATCAACAAGTG CCCCCTGCTGAAGCCATGGGCCTTGACTTTCTCCTATGGTCGAGCCCTGCAGGCCTCTGC TCTAAAGGCCTGGGGTGGGAAGAAGGAGAACCTGAAGGCAGCCCAGGAGGAGTACATCAA GCGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGG AGCCGCAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTA AGGCTGCTCCATCAACACTCCAGGCCCCTGCCTACCCACTTGCTATTGAAGAGGGGTCTT CAGGCTCTTTCCCATCACTCTTGCTGCTGCCCTCGTGTGCGGTGTTGTCTGTGAATGCTA AATCTGCCATCCCTTCCAGCCCACTGCCAATAAACAACTATTTAAGGGGGAAAAAAAAAA AAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC010568 unedited GGGCTCAGGATTTTGTAATACGACTTTACTATAGGGCGGCCGCGCAATTCGATCTGGTAC CGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCAGCTGAATAGGCTGCGTTC TCTTGGAACGCGCAGCAGAACGAGGTTCTGGTGACCCTAGCCGCGTTCGCTCCTTAGTCC TTTCGCCTACCCACCGGCGTACCAGGCAGACCCACCCCGTCCTGTGCCAGGAAAGCACTG CCACCGGCACCATGCCCCACCCATACCCAGCACTGACCCCGGAGCAGAAGAAGGAGCTGT CTGACATCGCTCACCGCATTGTGGCTCCGGGCAAGGGCATCCTGGCTGCAGATGAGTCCA CCGGAAGCATTGCCAAGCGCCTGCAGTCCATTGGCACCGAGAACACCGAGGAGAACAGGC GCTTCTACCGCCAGCTGCTGCTGACTGCAGACGACCGTGTGAATCCCTGCATTGGGGGGG TGATCCTCTTCCACGAGACACTGTACCAGAAGGCAGATGATGGACGTCCCTTCCCCCAAG TTATCAAGTCCAAGGGTGGTGTTGTGGGCATTAAGGTAGATAAGGGTGTGGTGCCCCTGG CAGGAACCAATGGCGAGACAACTACCCAGGGGCTGGATGGGCTGTCTGAACGCTGTGCCC AGTATAAGAAGGATGGAGCCGACTTTGCCAAGTGGCGCTGTGTGCTAAAGATTGGGGAAC ATACTCCCTCGGCCCTGGCCATCATGGGAAATGCCAATGTTCTGGGCCGTTATGCCAGCA TCTGCCAGCAGAATGGCATTGTACCCATTGTGGAGCCTNGAATTCTCCCTGATGGGGACC CATGACTGAAGCGCTGCCAGTATGTTACTGAGAAGGTCCTGGCNGCTGTGCTACAGGCTC TGAGCGC
Restriction Sites	NotI-NotI
ACCN	BC010568
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010568.1, AAH10568.1
RefSeq Size	1455 bp
Locus ID	226
Protein Families	Druggable Genome
Protein Pathways	Fructose and mannose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway
Gene Summary	This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017]

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