Carboxypeptidase A (CPA1) (BC005279) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	carboxypeptidase A1; carboxypeptidase A1 (pancreatic); CPA
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC005279 edited GACCTTCCCTCCCGGCAGCAGCATGCGGGGGTTGCTGGTGTTGAGTGTCCTGTTGGGGGC TGTCTTTGGCAAGGAGGACTTTGTGGGGCATCAGGTGCTCCGAATCTCTGTAGCCGATGA GGCCCAGGTACAGAAGGTGAAGGAGCTGGAGGACCTGGAGCACCTGCAGCTGGACTTCTG GCGGGGCCCTGCCCACCCTGGCTCCCCCATCGACGTCCGAGTGCCCTTCCCCAGCATCCA GGCGGTCAAGATCTTTCTGGAGTCCCACGGCATCAGCTATGAGACCATGATCGAGGACGT GCAGTCGCTGCTGGACGAGGAGCAGGAGCAGATGTTCGCCTTCCGGTCCCGGGCGCGCTC CACCGACACTTTTAACTACGCCACCTACCACACCCTGGAGGAGATCTATGACTTCCTGGA CCTGCTGGTGGCGGAGAACCCGCACCTTGTCAGCAAGATCCAGATTGGCAACACCTATGA AGGGCGTCCCATTTACGTGCTGAAGTTCAGCACGGGGGGCAGTAAGCGTCCAGCCATCTG GATCGACACGGGCATCCATTCCCGGGAGTGGGTCACCCAGGCCAGTGGGGTCTGGTTTGC AAAGAAGATCACTCAAGACTACGGGCAGGATGCAGCTTTCACCGCCATTCTCGACACCTT GGACATCTTCCTGGAGATCGTCACCAACCCTGATGGCTTTGCCTTCACGCACAGCACGAA TCGCATGTGGCGCAAGACTCGGTCCCACACAGCAGGCTCCCTCTGTATTGGCGTGGACCC CAACAGGAACTGGGACGCTGGCTTTGGGTTGTCCGGAGCCAGCAGTAACCCCTGCTCGGA GACTTACCGCGGCAAGTTTGCCAATTCCGAAGTGGAGGTCAAGTCCATTGTAGACTTTGT GAAGGACCATGGGAACATCAAGGCCTTCATCTCCATCCACAGCTACTCCCAGCTCCTCAT GTATCCCTATGGCTACAAAACAGAACCAGTCCCTGACCAGGATGAGCTGGATCAGCTTTC CAAGGCTGCTGTGACAGCCCTGGCCTCTCTCTACGGGACCAAGTTCAACTATGGCAGCAT CATCAAGGCAATTTATCAAGCCAGTGGAAGCACTATTGACTGGACCTACAGCCAGGGCAT CAAGTACTCCTTCACCTTCGAGCTCCGGGACACTGGGCGCTATGGCTTCCTGCTGCCAGC CTCCCAGATCATCCCCACAGCCAAGGAGACGTGGCTGGCGCTTCTGACCATCATGGAGCA CACCCTGAATCACCCCTACTGAGCTGACCCTTTGACACCCTTCTTGTCCTCCTCTCTGGC CCCATCCAGGCAACCAAATAAAGTTTGAGTGTACCAGGAACAGAATCCTGGGGCTTGCAG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC005279 unedited GATTTTGTAATACGACTGACTATAGGGCGGCCGCGAATTCGGCCATTACGGCCGGGGGAC CTTCCCTCCCGGCAGCAGCATGCGGGGGTTGCTGGTGTTGAGTGTCCTGTTGGGGGCTGT CTTTGGCAAGGAGGACTTTGTGGGGCATCAGGTGCTCCGAATCTCTGTAGCCGATGAGGC CCAGGTACAGAAGGTGAAGGAGCTGGAGGACCTGGAGCACCTGCAGCTGGACTTCTGGCG GGGCCCTGCCCACCCTGGCTCCCCCATCGACGTCCGAGTGCCCTTCCCCAGCATCCAGGC GGTCAAGATCTTTCTGGAGTCCCACGGCATCAGCTATGAGACCATGATCGAGGACGTGCA GTCGCTGCTGGACGAGGAGCAGGAGCAGATGTTCGCCTTCCGGTCCCGGGCGCGCTCCAC CGACACTTTTAACTACGCCACCTACCACACCCTGGAGGAGATCTATGACTTCCTGGACCT GCTGGTGGCGGAGAACCCGCACCTTGTCAGCAAGATCCAGATTGGCAACACCTATGAAGG GCGTCCCATTTACGTGCTGAAGTTCAGCACGGGGGGCAGTAAGCGTCCAGCCATCTGGAT CGACACGGGCATCCATTCCCGGGAGTGGGTCACCCAGGCCAGTGGGGTCTGGTTTGCAAA GAAGATCACTCAAGACTACGGGCAGGATGCAGCTTTCACCGCCATTCTCGACACCTTGGA CATCTTCCTGGAGAT
Restriction Sites	Please inquire
ACCN	BC005279
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005279.1, AAH05279.1
RefSeq Size	1410 bp
Locus ID	1357
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Protease, Secreted Protein
Gene Summary	This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. This enzyme is produced in the pancreas and preferentially cleaves C-terminal branched-chain and aromatic amino acids from dietary proteins. This gene and several family members are present in a gene cluster on chromosome 7. Mutations in this gene may be linked to chronic pancreatitis, while elevated protein levels may be associated with pancreatic cancer. [provided by RefSeq, Jan 2015]

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