SEC61G (BC009480) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SSS1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009480 edited CTGGCATTTTAGGTGTCGGTTGGGTAGGCAGTCATGGATCAGGTAATGCAGTTTGTTGAG CCAAGTCGGCAGTTTGTAAAGGACTCCATTCGGCTGGTTAAAAGATGCACTAAACCTGAT AGAAAAGAATTCCAGAAGATTGCCATGGCAACAGCAATAGGATTTGCTATAATGGGATTC ATTGGCTTCTTTGTGAAATTGATCCATATTCCTATTAATAACATCATTGTTGGTGGCTGA ATACATTTTGGAAGAGAGTTTTTCATCTTAGAGATTGGTGAACAAGTGTGAGGGTGTGAG AAACTCACAGAATACAAATTTGCCTGTATGTTTTGTGGGTTTTTTTTTTCCTTTCAAGAT GTTTTCTATTTCTAAATTAAAGTAATTTCAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009480 unedited NGAAGTTCGGATTTTGTAATACGACTTACTATAGGCGGACCGCGAATTCGCACGAGGCTG GCATTTTAGGTGTCGGTTGGGTAGGCAGTCATGGATCAGGTAATGCAGTTTGTTGAGCCA AGTCGGCAGTTTGTAAAGGACTCCATTCGGCTGGTTAAAAGATGCACTAAACCTGATAGA AAAGAATTCCAGAAGATTGCCATGGCAACAGCAATAGGATTTGCTATAATGGGATTCATT GGCTTCTTTGTGAAATTGATCCATATTCCTATTAATAACATCATTGTTGGTGGCTGAATA CATTTTGGAAGAGAGTTTTTCATCTTAGAGATTGGTGAACAAGTGTGAGGGTGTGAGAAA CTCACAGAATACAAATTTGCCTGTATGTTTTGTGGGTTTTTTTTTTCCTTTCAAGATGTT TTCTATTTCTAAATTAAAGTAATTTCAAAGTNNANAAAANNAAANAAAAAAAAAAAAAAA AAAAAAAAAAACCTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAAATC CCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTC CAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGT CCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATTGAACAAGGGGCAATTGGGAAAAA CAAACCGGAGGGCCCTGGGGGTCTATGGGGAACCAGCTGGGGTGCAGTGGCCCAATCTGG GTTAATGGAATCTCCGCCTCCGGGTTCAAGCGATTTTCTGCCCCACCTCCCGATTTTTGG GTTCCCGCCCGCCTAAACGGCTCACCAATTTTTGTTTTTTGGTAAAAAGGGTTTCCA
Restriction Sites	Please inquire
ACCN	BC009480
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009480.2, AAH09480.1
RefSeq Size	436 bp
RefSeq ORF	204 bp
Locus ID	23480
Protein Families	Transmembrane
Protein Pathways	Vibrio cholerae infection
Gene Summary	The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the gamma-subunit protein. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

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