HYI (BC019041) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	endothelial cell apoptosis protein E-CE1; HT036; hydroxypyruvate isomerase homolog; hydroxypyruvate isomerase homolog (E. coli); MGC20767; OTTHUMP00000008689
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC019041 edited GGCGCCGCTGCGCTTCTCCGCCAATCTGTCCTGGCTATTCCCCGAGCTCCCCGGCCTCCC CGCGCGGGTGCGGGCCGCGGGCAGCTCGGGCTTCGAGGCCGTCGAGGTGGCCTGGCCGTA CGCGGAGACGCCTGAGGCGCTGGCGCGCGCCGCGCGAGAAGCGGGGCTGCGGCTTGTACT GATCAACACGCCCCCGGGAGACCAAGAGAAGGGGGAAATGGGGCTGGGGGCCGTCCCCGG GAGACAGGCGGCCTTCCGAGAGGGACTGGAGCAGGCCGTGCGGTATGCCAAAGCCCTGGG CTGTCCCAGGATCCACCTGATGGCTGGCCGAGTACCCCAGGGAGCTGATCGAATAGCAGT CAAGGCTGAGATGGAGGCCGTTTTTCTGGAGAACCTGAGGCATGCAGCTGGGGTTTTGGC TCAGGAGGACCTCGTGGGACTGCTGGAGCCCATCAACACCCGCATCACTGATCCCCAGTA CTTCCTGGACACGCCCCAGCAGGCGGCAGCCATCTTACAGAAGGTAGGAAGACCCAACCT CCAATTACAAATGGACATATTCCACTGGCAGATCATGGATGGGAACCTGACAGGAAACAT CCGGGAGTTCCTGCCCATTGTTGGGCATGTGCAGGTGGCACAGGTCCCAGGCCGAGGGGA GCCCAGCAGCCCCGGAGAGCTGAATTTCCCCTATCTGTTTCAACTGCTGGAAGATGAAGG CTACAAAGGCTTCGTGGGCTGAGACACAGTAGAGGGCTTGAGTTGGCTACGTTCATACTG GGATAGGCGGGGCCACCCAGAGGCTGGCCAGTGAGGGCCCGCACACCACCCACGTGCCTC CAGACAGCGAGTGACATCCCATCTCCTCCTCTGCATTAAAGATGACCTGCTGAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC019041 unedited GGGTTAGATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGTACGCG GAGACGCCTGAGGCGCTGGCGCGCGCCGCGCGAGAAGCGGGGCTGCGGCTTGTACTGATC AACACGCCCCCGGGAGACCAAGAGAAGGGGGAAATGGGGCTGGGGGCCGTCCCCGGGAGA CAGGCGGCCTTCCGAGAGGGACTGGAGCAGGCCGTGCGGTATGCCAAAGCCCTGGGCTGT CCCAGGATCCACCTGATGGCTGGCCGAGTACCCCAGGGAGCTGATCGAATAGCAGTCAAG GCTGAGATGGAGGCCGTTTTTCTGGAGAACCTGAGGCATGCAGCTGGGGTTTTGGCTCAG GAGGACCTCGTGGGACTGCTGGAGCCCATCAACACCCGCATCACTGACCCCCAGTACTTC CTGGACACGCCCCAGCAGGCGGCAGCCATCTTACAGAAGGTAGGAAGACCCAACCTCCAA TTACAAATGGACATATTCCACTGGCAGATCATGGATGGGAACCTGACAGGAAACATCCGG GAGTTCCTGCCCATTGTTGGGCATGTGCAGGTGGCACAGGTCCCAGGCCGAGGGGAGCCC AGCAGCCCCGGAGAGCTGAATTTCCCCTATCTGTTTCAACTGCTGGAAAATGAAGGCTAC AAAGGCTTCGTGGGCTGTGAGTATCAGCCTCGAGGAGACACAGTAGAGGGCTTGAGTTGG CTACGTTCATACTGNGATAGGCGCGCCACCCAGAGGCTGGCCAGTGAGGGCCCGCACACC ACCCACGTGCCTNCAGACAGCGAGTGACATCCCATCTCCTCCTCTGCATTAAAGATGACC TGGCTGAAAAAAAANAAAAAACTCGAGCATCTATTTCGGTGCGGAGAAAGAGGTATGGAA TGGCCATGGT
Restriction Sites	Please inquire
ACCN	BC019041
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019041.2, AAH19041.1
RefSeq Size	953 bp
Locus ID	81888
Protein Pathways	Glyoxylate and dicarboxylate metabolism, Metabolic pathways
Gene Summary	This gene encodes a putative hydroxypyruvate isomerase, which likely catalyzes the conversion of hydroxypyruvate to 2-hydroxy-3-oxopropanoate, and may be involved in carbohydrate transport and metabolism. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

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