IGHG1 (BC014258) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC014258 edited GGCACCGAGGGTTTCTGTCCTCCACCATCATGGGGTCAACCGCCATCCTCGCCCTCCTCC TGGCTGTTCTCCAAGGAGTCTGTGCCGAGGTGCGCCTGGAGCAGTCTGGGACAGAGGTGA AAAAGCCGGGGGAGTCTCTGAAAATCTCCTGTCAGGCTTCTGGATTCACCTTTACCGACT ACTGGATCGGCTGGGTGCGCCAGCTGCCCGGGCAAGGCCTGGAGTGGATGGGCTTCATCG ATCGTCTTGACTCTAAAATAAGATATAACCCGTCCTTCCAAGGCCAAGTCACCATGTCAG CCGACACGTCGATAACCGCCGTCTACCTGCAGTGGAGCCGCCTGAAGGCCTCGGACACCG GCATCTATTATTGTGCGACCTCGGATACACCTCTGGACTCTTACTCCTTTGAATTTTGGG GCCAGGGAAGCCTCGTCATCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCC TGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGG ACTACTTCCCCGAACCGGTGACGGTGTCATGGAACTCAGGCGCCCTGACCAGCGGCGTGC ACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCG TGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCA ACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCAC CGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCA AGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCC ACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCA AGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCG TCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCC TCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGG TGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCC TGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGG AGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACA GCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGA TGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAAT GAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCTCTCGCGGTCGCACGAGGATGCTTG GCACGTACCCCCTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCACCCAGCGCT GCCCTGGGCCCCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC014258 unedited GGTTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGTAATTCGCACCGAGGGGTTTC TCGTCCTCCACCATCATGGGGTCAACCGCCATCCTCGCCCTCCTCCTGGCTGTTCTCCAA GGAGTCTGTGCCGAGGTGCGCCTGGAGCAGTCTGGGACAGAGGTGAAAAAGCCGGGGGAG TCTCTGAAAATCTCCTGTCAGGCTTCTGGATTCACCTTTACCGACTACTGGATCGGCTGG GTGCGCCAGCTGCCCGGGCAAGGCCTGGAGTGGATGGGCTTCATCGATCGTCTTGACTCT AAAATAAGATATAACCCGTCCTTCCAAGGCCAAGTCACCATGTCAGCCGACACGTCGATA ACCGCCGTCTACCTGCAGTGGAGCCGCCTGAAGGCCTCGGACACCGGCATCTATTATTGT GCGACCTCGGATACACCTCTGGACTCTTACTCCTTTGAATTTTGGGGCCAGGGAAGCCTC GTCATCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCC AAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAA CCGGTGACGGTGTCATGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCT GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGAC AAGAAAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCT GAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGACACCCTCATGA TCTCCCGGACCCCTGAGGTCACATGCGTTGGTGGTGGACGTGAGCCACGAAGAAC
Restriction Sites	NotI-NotI
ACCN	BC014258
Insert Size	1600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	The ORF of this clone has been fully sequenced and found to be a perfect match to BC014258.1.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC014258.1
RefSeq Size	1602 bp
Locus ID	3500

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