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AMBRA1 (NM_017749) Human Untagged Clone
CAT＃: SC125736
AMBRA1 (untagged)-Human autophagy/beclin-1 regulator 1 (AMBRA1)
CNY 12,624.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DCAF3; WDR94 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_017749 edited
ATGAAGGTTGTCCCAGAAAAGAATGCTGTCCGGATACTCTGGGGGCGAGAACGGGGTGCTCGGGCCATGG GAGCTCAGCGGCTTCTGCAGGAGCTGGTAGAGGATAAAACCCGGTGGATGAAATGGGAGGGCAAGAGAGT AGAACTGCCGGATAGTCCACGCTCTACCTTCTTATTGGCCTTCAGCCCAGACAGGACTCTCTTAGCCTCC ACCCATGTGAACCATAATATCTATATTACGGAGGTGAAGACTGGCAAGTGTGTTCATTCCCTGATTGGAC ACCGCCGTACTCCATGGTGTGTCACTTTTCATCCCACCATCTCAGGCCTTATTGCTTCTGGCTGCCTAGA TGGGGAGGTTAGGATTTGGGATTTACACGGTGGCAGTGAAAGCTGGTTCACAGATAGCAACAATGCCATT GCCTCCCTGGCTTTCCACCCTACGGCTCAGCTCCTGCTGATTGCCACTGCCAATGAGATCCACTTCTGGG ACTGGAGTCGACGGGAACCCTTTGCTGTGGTGAAGACAGCTAGTGAGATGGAACGGGTCCGTCTGGTGAG ATTTGATCCACTTGGACACTACTTACTCACAGCAATTGTTAACCCCTCTAATCAACAGGGTGATGACGAA CCAGAGATCCCCATAGATGGAACAGAATTATCCCACTACCGTCAGCGTGCCCTCCTGCAATCACAGCCAG TTCGCCGGACGCCTCTCCTCCACAATTTCCTGCACATGCTGTCCTCCCGCTCTTCTGGCATCCAGACCGA GCCCTTCCATCCCCCGGAGCAGGCCTCGTCAACGCAGCAGGACCAGGGCCTCCTGAACCGGCCGTCTGCC TTCAGTACAGTCCAGAGCAGCACTGCCGGCAACACGCTCCGCAACCTCAGTCTGGGTCCTACCCGCCGCT CTTTGGGAGGGCCTCTGTCTAGCCACCCTTCTAGGTATCACCGAGAAATAGCTCCTGGGTTGACAGGATC TGAGTGGACCCGGACAGTACTCAGTCTGAACTCCCGCTCTGAGGCGGAATCCATGCCCCCGCCCAGAACC AGTGCCTCTTCGGTGAGTTTGCTGTCTGTGCTGAGACAGCAGGAAGGTGGCTCTCAGGCATCTGTGTACA CTTCAGCCACAGAAGGGAGGGGTTTTCCGGCATCAGGGTTGGCAACTGAGTCAGATGGAGGGAATGGCTC CAGCCAAAACAACTCGGGCAGCATTCGCCATGAGCTTCAGTGTGACCTGAGACGCTTCTTTCTGGAGTAT GACCGGCTTCAGGAGCTGGATCAGAGCCTGAGTGGGGAAGCTCCCCAGACCCAACAGGCCCAGGAAATGC TCAACAATAACATTGAATCTGAGAGGCCAGGCCCTTCCCACCAGCCCACCCCACACAGCAGTGAGAACAA CTCCAACCTGTCCCGTGGCCACTTGAATCGCTGTCGTGCTTGCCACAATCTCCTGACCTTCAACAACGAT ACCCTGCGCTGGGAAAGAACCACACCTAACTACTCCTCTGGCGAGGCTAGTTCCTCTTGGCAGGTCCCCA GCTCCTTTGAGAGTGTGCCATCAAGTGGCAGCCAGTTGCCACCTCTCGAGCGGACTGAGGGCCAAACGCC CAGCTCCAGCAGGCTGGAGTTGAGCAGCTCTGCTAGTCCGCAGGAGGAGAGGACTGTGGGGGTGGCCTTT AACCAGGAGACAGGCCACTGGGAAAGAATTTACACCCAGTCCAGCAGATCTGGAACTGTGTCACAGGAGG CCTTACATCAGGATATGCCTGAGGAGAGCTCTGAGGAGGATTCACTCAGGAGGAGGCTGCTGGAATCTTC CCTCATTTCATTATCCCGTTATGATGGAGCAGGATCCAGAGAGCACCCAATTTACCCAGACCCAGCGAGA TTATCTCCTGCTGCATACTACGCCCAGAGGATGATCCAGTATCTCTCACGGAGAGACAGTATTCGCCAGC GCTCCATGCGCTACCAACAGAACCGTCTCCGTTCTTCCACCTCCTCCTCTTCCTCAGACAACCAGGGTCC ATCAGTAGAGGGAACCGACTTGGAATTTGAGGACTTTGAGGACAATGGTGACAGATCCAGGCACCGAGCT CCACGCAATGCCCGGATGTCTGCACCTTCGCTTGGACGCTTTGTCCCAAGGCGTTTCTTGCTGCCTGAGT ACTTGCCTTATGCTGGGATTTTTCATGAACGTGGACAGCCTGGCTTGGCTACTCACTCTTCTGTTAACAG GGTCCTGGCAGGGGCAGTGATCGGTGATGGACAGTCTGCTGTGGCCAGTAACATTGCCAATACTACCTAC CGGCTCCAGTGGTGGGACTTCACTAAGTTTGACCTCCCTGAAATCAGTAATGCTTCCGTGAATGTGCTGG TGCAGAACTGCAAGATCTACAATGATGCCAGCTGTGACATTTCTGCAGATGGCCAGCTCCTGGCAGCTTT CATCCCCAGCAGCCAGAGGGGCTTTCCTGATGAAGGCATCCTGGCAGTGTACTCCCTGGCCCCCCATAAC CTGGGCGAAATGCTCTACACCAAGCGATTTGGTCCCAATGCCATTTCGGTGAGCCTGTCCCCAATGGGCA GATATGTAATGGTGGGCTTGGCCTCACGAAGGATCCTGCTGCACCCCTCCACAGAGCACATGGTGGCCCA GGTCTTCAGGCTGCAACAGGCCCATGGTGGAGAGACCTCCATGAGGAGAGTTTTCAACGTCCTTTATCCC ATGCCTGCCGACCAGCGGAGACATGTCAGTATCAACTCTGCCCGTTGGCTGCCTGAGCCAGGGCTTGGCT TGGCCTATGGTACTAACAAAGGAGACCTGGTGATCTGCCGACCAGAGGCCTTAAACTCTGGTGTTGAGTA CTACTGGGACCAGCTGAACGAGACGGTCTTCACTGTCCATTCCAACAGCAGGAGCAGCGAGCGGCCTGGA ACCAGCAGAGCCACATGGAGGACAGACAGAGACATGGGGCTGATGAATGCCATTGGGCTTCAGCCCCGGA ACCCTGCCACCTCAGTGACATCTCAGGGCACCGAGACTCTGGCCCTTCAGCTGCAGAATGCCGAAACACA GACTGAGAGGGAGGTGCCGGAGCCAGGGACAGCCGCCTCAGGTCCTGGTGAAGGTGAGGGTTCAGAGTAT GGTGCCAGTGGAGAAGATGCGCTCAGCAGGATCCAGAGGCTGATGGCGGAGGGCGGCATGACAGCCGTGG TGCAGCGGGAGCAGAGCACCACCATGGCCTCCATGGGCGGCTTCGGCAACAACATCATCGTCAGCCACCG CATTCACCGCAGCTCTCAGACGGGCACTGAACCTGGTGCCGCCCACACCTCCTCACCCCAGCCCTCCACC TCTCGGGGACTGCTCCCAGAGGCCGGGCAACTGGCAGAGCGAGGCCTAAGCCCCCGGACAGCTTCCTGGG ACCAGCCTGGTACCCCTGGGCGGGAGCCAACCCAGCCAACCCTGCCCTCTTCCTCCCCTGTCCCCATTCC TGTTTCCCTTCCCAGCGCTGAGGGACCAACCGTCCACTGCGAGTTGACCAATAACAACCACCTTCTGGAT GGTGGCAGCAGCAGGGGGGACGCTGCAGGCCCTAGGGGAGAACCACGGAACAGGTAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017749 |
| Insert Size | 3600 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017749.2, NP_060219.2 |
| RefSeq Size | 5044 bp |
| RefSeq ORF | 3627 bp |
| Locus ID | 55626 |
| UniProt ID | Q9C0C7 |
| Gene Summary | Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2 and 8 both encode the same isoform (2). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Parkin Interacts with Ambra1 to Induce Mitophagy
,Cindy Van Humbeeck, Tom Cornelissen, Hilde Hofkens, Wim Mandemakers, Kris Gevaert, Bart De Strooper, and Wim Vandenberghe,
J. Neurosci., Jul 2011; 31: 10249 - 10261.
[AMBRA1]
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Other Versions
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| RC206255L1 | Lenti ORF clone of Human autophagy/beclin-1 regulator 1 (AMBRA1), Myc-DDK-tagged |
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| RC206255L2 | Lenti ORF clone of Human autophagy/beclin-1 regulator 1 (AMBRA1), mGFP tagged |
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| RC206255L3 | Lenti ORF clone of Human autophagy/beclin-1 regulator 1 (AMBRA1), Myc-DDK-tagged |
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| RC206255L4 | Lenti ORF clone of Human autophagy/beclin-1 regulator 1 (AMBRA1), mGFP tagged |
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| RG206255 | AMBRA1 (tGFP-tagged) - Human autophagy/beclin-1 regulator 1 (AMBRA1) |
CNY 14,216.00 |
