GCP4 (TUBGCP4) (BC000966) Human Untagged Clone

CAT#: SC125678

TUBGCP4 (untagged)-Human gamma tubulin ring complex protein (76p gene), mRNA (cDNA clone MGC:4930 IMAGE:3446803), complete cds



CNY 2,950.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-TUBGCP4 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms 76P; FLJ14797; gamma tubulin ring complex protein (76p gene); GCP4; tubulin, gamma complex associated protein 4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for BC000966 edited
ATGGAATCTACCTGCGGGCCTTCTGCACAGGGCTGGATTCTGTTTTGCAGCCTTATCGCC
AAGCACTGCTTGATTTGGAACAAGAGTTCCTGGGTGATCCCCATCTCTCCATATCACATG
TCAACTACTTCCTAGACCAGTTCCAGCTTCTTTTTCCCTCTGTGATGGTTGTAGTAGAAC
AAATTAAAAGTCAAAAGATTCATGGTTGTCAAATCCTGGAAACAGTCTACAAACACAGCT
GTGGGGGGTTGCCTCCTGTTCGAAGTGCACTGGAAAAAATCCTGGCCGTTTGTCATGGGG
TCATGTATAAACAGCTCTCAGCCTGGATGCTCCATGGACTCCTCTTGGACCAGCATGAAG
AATTCTTTATCAAACAGGGGCCATCTTCTGGTAATGTCAGTGCCCAGCCAGAAGAGGACG
AGGAGGATCTGGGCATTGGGGGACTGACAGGAAAACAACTGAGAGAACTGCAGGACTTGC
GCCTGATTGAGGAAGAGAACATGCTGGCACCATCTCTGAAGCAGTTTTCCCTACGAGTGG
AGATTTTGCCATCCTACATTCCAGTGAGGGTTGCTGAAAAAATCCTATTTGTTGGAGAAT
CTGTCCAGATGTTTGAGAATCAAAATGTGAACCTGACTAGAAAAGGATCCATTTTGAAAA
ACCAGGAAGACACTTTTGCTGCAGAGCTGCACCGTCTCAAGCAGCAGCCACTCTTCAGCT
TGGTGGACTTTGAACAGGTGGTGGATCGCATTCGCAGCACTGTGGCTGAGCATCTCTGGA
AGTTGATGGTAGAAGAATCCGATTTACTGGGTCAGCTGAAGATCATTAAAGACTTTTACC
TTCTGGGACGTGGAGAACTGTTTCAGGCCTTCATTGACACAGCTCAACACATGTTGAAAA
CACCACCCACTGCAGTAACTGAGCATGATGTGAATGTGGCCTTTCAACAGTCAGCACACA
AGGTATTGCTAGATGATGACAACCTTCTCCCTCTGTTGCACTTGACAATCGAGTATCACG
GAAAGGAGCACAAAGCAGATGCTACTCAGGCAAGAGAAGGGCCTTCTCGGGAAACTTCTC
CCCGGGAAGCCCCTGCATCTGGCTGGGCAGCCCTAGGTCTTTCCTACAAAGTACAGTGGC
CACTACATATTCTCTTCACCCCAGCTGTCCTGGAAAAAAATAGACAATTTTAAAAACCAA
ACAGAATGGGACTGTCTTCTGCAAGCCTACCTACAAACAGGTACAATGTTGTTTTTAAGT
ACTTACTGAGTGTGCGCCGGGTGCAAGCTGAGCTGCAGCACTGCTGGGCCCTACAAATGC
AGCGCAAGCACCTCAAGTCGAACCAGACTGATGCAATCAAGTGGCGCCTAAGAAATCACA
TGGCATTTTTGGTGGATAATCTTCAGTACTATCTCCAGGTAGATGTGTTGGAGTCTCAGT
TCTCCCAGCTGCTTCATCAGATCAATTCTACCCGAGACTTTGAAAGCATCCGATTGGCTC
ATGACCACTTCCTGAGCAATTTGCTGGCTCAATCCTTTATCCTATTGAAACCTGTGTTTC
ACTGCCTGAATGAAATCCTAGATCTCTGTCACAGTTTTTGTTCGCTGGTCAGTCAGAACC
TAGGCCCACTGGATGAGCGTGGAGCCGCCCAGCTGAGCATTCTCGTGAAGGGCTTTAGCC
GCCAGTCTTCACTCCTGTTCAAGATTCTCTCCAGTGTTCGGAATCATCAGATCAACTCAG
ATTTGGCTCAACTACTGTTACGACTAGATTATAACAAATACTATACCCAGGCTGGTGGAA
CTCTGGGCAGTTTCGGGATGTGAAAATTTCTGGCTCATAAATTGAAATAACAGCCACGTT
CCCAAGGTTGTAACAGAAGATTCAAAACATCCCATTCTAGCCACACACAAATAAATATCT
GCGGCTTAAAAAAAAAAAAAAAAAA
>OriGene 5' read for BC000966 unedited
GAGTTAAATATTTGTATACCACTCACTATAGGCGGCCGCGNATTCANATCTGGTACCGGT
CCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGATGGAATCTACCTGCGGGCCTTCT
GCACAGGGCTGGATTCTGTTTTGCAGCCTTATCGCCAAGCACTGCTTGATTTGGAACAAG
AGTTCCTGGGTGATCCCCATCTCTCCATATCACATGTCAACTACTTCCTAGACCAGTTCC
AGCTTCTTTTTCCCTCTGTGATGGTTGTAGTAGAACAAATTAAAAGTCAAAAGATTCATG
GTTGTCAAATCCTGGAAACAGTCTACAAACACAGCTGTGGGGGGTTGCCTCCTGTTCGAA
GTGCACTGGAAAAAATCCTGGCCGTTTGTCATGGGGTCATGTATAAACAGCTCTCAGCCT
GGATGCTCCATGGACTCCTCTTGGACCAGCATGAAGAATTCTTTATCAAACAGGGGCCAT
CTTCTGGTAATGTCAGTGCCCAGCCAGAAGAGGACGAGGAGGATCTGGGCATTGGGGGAC
TGACAGGAAAACAACTGAGAGAACTGCAGGACTTGCGCCTGATTGAGGAAGAGAACATGC
TGGCACCATCTCTGAAGCAGTTTTCCCTACGAGTGGAGATTTTGCCATCCTACATTCCAG
TGAGGGTTGCTGAANAAATCCTATTTGTTGGAGAATCTGTCCAGATGTTTGAGAATCAAA
ATGTGAACCTGACTAGAAAAAGATCCCATTTTGAAAAACCAGNAAGACACTTTTTGCTGC
AGAGCTGCACCGTCTCAAGCAGCAGCCACTCTTCAGCTTGGTGGACTTTGAACAGGTGGT
GGGATCGCATTCGCAGCACTGTGGCTGAGCATCTCTGGAAATTGATGGTAGAAGAA
Restriction Sites NotI-NotI     
ACCN BC000966
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC000966.2, AAH00966.1
RefSeq Size 1945 bp
Locus ID 27229
Gene Summary This gene encodes a component of the gamma-tubulin ring complex, which is required for microtubule nucleation. In mammalian cells, the protein localizes to centrosomes in association with gamma-tubulin. Crystal structure analysis revealed a structure composed of five helical bundles arranged around conserved hydrophobic cores. An exposed surface area located in the C-terminal domain is essential and sufficient for direct binding to gamma-tubulin. Mutations in this gene that alter microtubule organization are associated with microcephaly and chorioretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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