AP2M1 (BC013796) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AP50; CLAPM1; mu2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC013796 edited GGGCCGGCAGAGCAGGGGGCCGAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGA GACTGATCTGCCGCCATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTC ATCTCCCGAGTCTACCGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAAT GTTATCCATGCCCGGCAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTC TTCCACGTTAAGCGGTCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCT GCCATGGTCTTCGAATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAG ATCAGCGAGGAAAACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATT CTAGACTTTGGCTACCCACAGAATTCCGAGACAGGCGCGCTGAAAACCTTCATCACGCAG CAGGGCATCAAGAGTCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGG CAGATTGGCTGGCGGCGAGAGGGTATCAAGTATCGTCGGAATGAGCTCTTCCTGGATGTG CTGGAGAGTGTGAACCTGCTCATGTCCCCACAAGGGCAGGTGCTGAGTGCCCATGTGTCG GGCCGGGTGGTGATGAAGAGCTACCTGAGTGGCATGCCTGAATGCAAGTTTGGGATGAAT GACAAGATTGTTATTGAAAAGCAGGGCAAAGGCACAGCTGATGAAACAAGCAAGAGCGGG AAGCAATCAATTGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGCAAGTTT GACTCTGAACGCAGCATCAGCTTTATCCCGCCAGATGGAGAGTTTGAGCTTATGAGGTAT CGCACAACCAAGGACATCATCCTTCCCTTCCGGGTGATCCCGCTAGTGCGAGAAGTGGGA CGCACCAAACTGGAGGTCAAGGTGGTCATCAAGTCCAACTTTAAACCCTCACTGCTGGCT CAGAAGATTGAGGTGAGGATCCCAACCCCACTGAACACAAGCGGGGTGCAGGTGATCTGC ATGAAGGGGAAGGCCAAGTACAAGGCCAGCGAGAATGCCATCGTGTGGAAGATCAAGCGC ATGGCAGGCATGAAGGAATCGCAGATCAGCGCAGAGATTGAGCTTCTGCCTACCAACGAC AAGAAGAAATGGGCTCGACCCCCCATTTCCATGAACTTTGAGGTGCCATTCGCACCCTCT GGCCTCAAGGTGCGCTACTTGAAGGTGTTTGAACCGAAGCTGAACTACAGCGACCATGAT GTCATCAAATGGGTGCGCTACATTGGCCGCAGTGGCATTTATGAAACTCGCTGCTAGCTG CCACTAGGCAGCTAGCCCACCTCCCCAGCCACCCTCCTCCACAGGTCCAGGTGCCGCTCC CTCCCCCACCACACATCAGTGTCTCCTCCCTCCTGCTTTGCTGCCTTCCCTTTGCACCAG CCCGAGTCTAGGTCTGGGCCAAGCACATTACAAGTGGGACCGGTGGAGCAGCCCCTGGGC TCCCTGGGCAGGGGAGTTCTGAGGCTCCTGCTCTCCCATCCACCTGTCTGTCCTGGCCTA ATGCCAGGCTCTGAGTTCTGTGACCAAAGCCAGGTGGGTTCCCTTTCCTTCCCACCCCTG TGGCCACAGCTCTGGAGTGGGAGGGTTGGTTGCCCCTCACCTCAGAGCTCCCCCAAAGGC CAGTAATGGATCCCCGGCCTCAGTCCCTACTCTGCTTTGGGATAGTGTGAGCTTCATTTT GTACACGTGTGACTTCGTCCAGTTACAAACCCAATAAACTCTGTAGAGTGGAAAAAAAAA AAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC013796 unedited TTCTGGATTTGTAATACGACTCTATATAGGGCGGCCGCGNAATCAAATCTGGTACCGGTC CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCCCACGCGTCCGGGGCCGGCAGAGC AGGGGGCCGAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGAGACTGATCTGCCG CCATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTCATCTCCCGAGTCT ACCGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAATGTTATCCATGCCC GGCAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTCTTCCACGTTAAGC GGTCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCTGCCATGGTCTTCG AATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAGATCAGCGAGGAAA ACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATTCTAGACTTTGGCT ACCCACAGAATTCCGAGACAGGCGCGCTGAANACCTTCATCACGCAGCAGGGCATCAAGA GTCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGGCAGATTGGCTGGC GGCGAGAGGGTATCAAGTATCGTCNGAATGAGCTCTTCCTGGATGTGCTGGAGAGTGTGA ACCTGCTCATGTCCCCACAGGGGCAGGTGCTGAGTGCCCATGTGTCNGGCCGGGTGGTGA TGAAGAGCTACCTGAGTGGCATGCCTGAAATGCAGGTTGGGGATGAATNGACAGATTGTT ATTGAAAAGCAGGGCANAGGCACAGCTGATGAAANNCAGCAGAGCGGGGAGCAATCCATT GCCATTGATGACTGCACCCTTCACCANTGTGTGCGACTCANCAGTTTN
Restriction Sites	Please inquire
ACCN	BC013796
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013796.1, AAH13796.1
RefSeq Size	1867 bp
Locus ID	1173
Protein Families	Druggable Genome
Protein Pathways	Endocytosis, Huntington's disease
Gene Summary	This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]

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