Myosin Light Chain 2 (MYL2) (BC015821) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	cardiac ventricular myosin light chain 2; CMH10; DKFZp779C0562; MLC2; myosin, light polypeptide 2, regulatory, cardiac, slow; myosin light chain 2; myosin regulatory light chain 2, ventricular/cardiac muscle isoform; regulatory light chain; RLC of myosin
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC015821 edited GGGGGGCGGAGTGTGGAATTCTTCTCGGGAGGCAGTGCTGGGTCCTTTCCACCATGGCAC CTAAGAAAGCAAAGAAGAGAGCCGGGGGCGCCAACTCCAACGTGTTCTCCATGTTCGAAC AGACCCAAATCCAGGAATTTAAGGAGGCCTTCACTATCATGGACCAGAACAGGGATGGCT TCATTGACAAGAACGATCTGAGAGACACCTTTGCTGCCCTTGGGCGAGTGAACGTGAAAA ATGAAGAAATTGATGAAATGATCAAGGAGGCTCCGGGTCCAATTAACTTTACTGTGTTCC TCACAATGTTTGGGGAGAAACTTAAGGGAGCGGACCCTGAGGAAACCATTCTCAACGCAT TCAAAGTGTTTGACCCTGAAGGCAAAGGGGTGCTGAAGGCTGATTACGTTCGGGAAATGC TGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGAGGTTGACCAGATGTTCGCCGCCTTCC CCCCTGACGTGACTGGCAACTTGGACTACAAGAACCTGGTGCACATCATCACCCACGGAG AAGAGAAGGACTAGGAGGGGGCTCGCTGCTGCGCCCTGGGCTCGTCTTTGCAGAGTGGTC CCTGCCCTCATCTCTCTCCCCCGAGTACCGCCTCTGTCCCTACCTTGTCTGTTAGCCATG TGGCTGCCCCATTTATCCACCTCCATCTTCTTTGCAGCCTGGGTGGCTATGGGTACTTCG TGGCCGCACATCCTACAGTTGGAAATCCATCCAGAGGCCATGTTCCAATAAACAGGAGGT CGTGTATTTGGTCACGACATTTCTCTGATAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC015821 unedited NCCCGTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATCTTCTCGGGNAGCAGT GCTGGGTCCTTTCCACCATGGCACCTAAGAAAGCAAAGAAGAGAGCCGGGGGCGCCAACT CCAACGTGTTCTCCATGTTCGAACAGACCCAAATCCAGGAATTTAAGGAGGCCTTCACTA TCATGGACCAGAACAGGGATGGCTTCATTGACAAGAACGATCTGAGAGACACCTTTGCTG CCCTTGGGCGAGTGAACGTGAAAAATGAAGAAATTGATGAAATGATCAAGGAGGCTCCGG GTCCAATTAACTTTACTGTGTTCCTCACAATGTTTGGGGAGAAACTTAAGGGAGCGGACC CTGAGGAAACCATTCTCAACGCATTCAAAGTGTTTGACCCTGAAGGCAAAGGGGTGCTGA AGGCTGATTACGTTCGGGAAATGCTGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGAGG TTGACCAGATGTTCGCCGCCTTCCCCCCTGACGTGACTGGCAACTTGGACTACAAGAACC TGGTGCACATCATCACCCACGGAGAAGAGAAGGACTAGGAGGGGGCTCGCTGCTGCGCCC TGNGCTCGTCTTTGCAGAGTGGTCCCTGCCCTCATCTCTCTCCCCCGAGTACCGCCTCTG TCCCTACCTTGTCTGTTAGCCATGTGGCTGCCCCATTTATCCACCTCCATCTTCTTTGCA GCCTGGGTGGCTATGGGTACTTCGTGGCCGCACATCCTACAGTTGGAAATCCATCCAGAG GCCATGTTCCANATAACAGGGAGTCGTGTATTTGGTCACGACATTTCTCTGATNNNNAAA AANNNNNNNNNNNAAAACATGTCGGCCCGCTCGGCCCTCGAGAAGCTTTCTAGATTGCGG CCGCGG
Restriction Sites	Please inquire
ACCN	BC015821
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC015821.1, AAH15821.1
RefSeq Size	834 bp
Locus ID	4633
Protein Pathways	Cardiac muscle contraction, Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction
Gene Summary	Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

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