H1oo (H1FOO) (BC047943) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	H1 histone family, member O, oocyte-specific; MGC50807; oocyte-specific histone H1; osH1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC047943 edited GCGTCACAGCAGGACCAGGCGGCTAAGCAGGGAGAAGAGCCAGAGGGGCGTCAGGTGTCT GATGCGGTGTGCTGGCAGTTGAGGGGTGAAGTTAGTTCCCAAGCACAAGAAGAAAATCCA GCCCAGGAAGATGGCCCCCGCGACGGCTCCCAGGAGAGCGGGTGAGGCCAAGGGGAAGGG CCCCAAGAAACCAAGTGAGGCCAAGGAGGACCCTCCCAACGTGGGCAAGGTGAAAAAGGC AGCCAAGAGGCCAGCAAAGGTGCAGAAGCCTCCTCCCAAGCCAGGCGCAGCCACAGAGAA GGCTCGCAAGCAAGGCGGCGCGGCCAAGGACACCAGGGCACAGTCGGGAGAGGCTAGGAA GGTGCCCCCCAAGCCAGACAAGGCCATGCGGGCACCTTCCAGTGCTGGTGGGCTCAGCAG GAAGGCAAAGGCCAAAGGCAGCAGGAGCAGCCAAGGAGATGCTGAGGCCTACAGGAAAAC CAAAGCTGAGAGTAAGAGTTCAAAACCCACGGCCAGCAAGGTCAAGAATGGTGCTGCTTC CCCGACCAAAAAGAAGGTGGTGGCCAAGGCCAAGGCCCCTAAAGCTGGGCAGGGGCCAAA CACCAAGGCTGCTGCTCCTGCTAAGGGCAGTGGGTCCAAGGTGGTACCTGCACATTTGTC CAGGAAGACAGAGGCCCCCAAGGGCCCTAGAAAGGCTGGGCTGCCCATCAAGGCCTCATC ATCCAAAGTGTCCAGCCAGAGGGCTGAAGCTTAGGGCCAGAGGCAGGGGCGGAGAGAGAC CGAGCCTCTGCCCTAGTTTTTATTCTTCAACTAACCACTGCTCTATTTATTTCATTGTAA GCTATTTATCAATAAAGACTTTTGTTTCTTTTTCCTCACAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC047943 unedited NGGGTCAGAATTTGTATACGACTCATATAGGCGGACCGCGATTCCCGGGNAGCGTCACAG CAGGACCAGGCGGCTAAGCAGGGAGAAGAGCCAGAGGGGCGTCAGGTGTCTGATGCGGTG TGCTGGCAGTTGAGGGGTGAAGTTAGTTCCCAAGCACAAGAAGAAAATCCAGCCCAGGAA GATGGCCCCCGCGACGGCTCCCAGGAGAGCGGGTGAGGCCAAGGGGAAGGGCCCCAAGAA ACCAAGTGAGGCCAAGGAGGACCCTCCCAACGTGGGCAAGGTGAAAAAGGCAGCCAAGAG GCCAGCAAAGGTGCAGAAGCCTCCTCCCAAGCCAGGCGCAGCCACAGAGAAGGCTCGCAA GCAAGGCGGCGCGGCCAAGGACACCAGGGCACAGTCGGGAGAGTTAGGGAGAAGGTGCCC CCCAAGCCAGACAAGGCCATGCGGGCACCTTCCAGTGCTGGTGGGCTCAGCAGGAAGGCA AAGGCCAAAGGCAGCAGGAGCAGCCAAGGAGATGCTGAGGCCTACAGGAAAACCAAAGCT GAGAGTAAGAGTTCAAAACCCACGGCCAGCAAGGTCAAGAATGGTGCTGCTTCCCCGACC AAAAAGAAGGTGGTGGCCAAGGCCAAGGCCCCTAAAGCTGGGCAGGGGCCAAACACCAAG GCTGCTGCTCCTGCTAAGGGCAGTGGGTCCAAGGTGGTACCTGCACATTTGTCCAGGAAG ACAGAGGCCCCCAAGGGCCCTAGAAAGGCTGGGCTGCCCATCAAGGCCTCATCATCCAAA GTGTCCAGCCAGAAGGCTGAAGCTAAGGGCCGNAGCANGGGCGGAGAGAGACCCGAGCTC TGCCCTAGTTTTTATTCTTCACTAACACTGCTCTATTTATTTCATTGTNNAGCTATTATC ANTAAGGACTTTGGGTTTCTTTTTCCTCACAAAAA
Restriction Sites	NotI-NotI
ACCN	BC047943
Insert Size	1000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC047943.1, AAH47943.1
RefSeq Size	997 bp
Locus ID	132243
Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a replication-independent histone that is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The related mouse gene is expressed only in oocytes. [provided by RefSeq, Oct 2015]

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