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Caspase 8 (CASP8) (NM_033355) Human Untagged Clone
CAT＃: SC125549
CASP8 (untagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant B
CNY 3,656.00
CNY 7,700.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_033355, the custom clone sequence may differ by one or more nucleotides
ATGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAACTGGACAGTGAAGATCTGGCCTCCCTCAAGT TCCTGAGCCTGGACTACATTCCGCAAAGGAAGCAAGAACCCATCAAGGATGCCTTGATGTTATTCCAGAG ACTCCAGGAAAAGAGAATGTTGGAGGAAAGCAATCTGTCCTTCCTGAAGGAGCTGCTCTTCCGAATTAAT AGACTGGATTTGCTGATTACCTACCTAAACACTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCAG GCAGGGCTCAAATTTCTGCCTACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGCAGATCAGAATT GAGGTCTTTTAAGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTGGATGATGACATGAACCTGCTG GATATTTTCATAGAGATGGAGAAGAGGGTCATCCTGGGAGAAGGAAAGTTGGACATCCTGAAAAGAGTCT GTGCCCAAATCAACAAGAGCCTGCTGAAGATAATCAACGACTATGAAGAATTCAGCAAAGAGAGAAGCAG CAGCCTTGAAGGAAGTCCTGATGAATTTTCAAATGGGGAGGAGTTGTGTGGGGTAATGACAATCTCGGAC TCTCCAAGAGAACAGGATAGTGAATCACAGACTTTGGACAAAGTTTACCAAATGAAAAGCAAACCTCGGG GATACTGTCTGATCATCAACAATCACAATTTTGCAAAAGCACGGGAGAAAGTGCCCAAACTTCACAGCAT TAGGGACAGGAATGGAACACACTTGGATGCAGGGGCTTTGACCACGACCTTTGAAGAGCTTCATTTTGAG ATCAAGCCCCACGATGACTGCACAGTAGAGCAAATCTATGAGATTTTGAAAATCTACCAACTCATGGACC ACAGTAACATGGACTGCTTCATCTGCTGTATCCTCTCCCATGGAGACAAGGGCATCATCTATGGCACTGA TGGACAGGAGGCCCCCATCTATGAGCTGACATCTCAGTTCACTGGTTTGAAGTGCCCTTCCCTTGCTGGA AAACCCAAAGTGTTTTTTATTCAGGCTTGTCAGGGGGATAACTACCAGAAAGGTATACCTGTTGAGACTG ATTCAGAGGAGCAACCCTATTTAGAAATGGATTTATCATCACCTCAAACGAGATATATCCCGGATGAGGC TGACTTTCTGCTGGGGATGGCCACTGTGAATAACTGTGTTTCCTACCGAAACCCTGCAGAGGGAACCTGG TACATCCAGTCACTTTGCCAGAGCCTGAGAGAGCGATGTCCTCGAGGCGATGATATTCTCACCATCCTGA CTGAAGTGAACTATGAAGTAAGCAACAAGGATGACAAGAAAAACATGGGGAAACAGATGCCTCAGCCTAC TTTCACACTAAGAAAAAAACTTGTCTTCCCTTCTGATTGA >OriGene 5' read for NM_033355 unedited
AGATTTGGAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGTTGTTCA GACTGAGCTTCCTGCCTGCCTGTACCCCGCCAACAGCTTCAGAAGAAGGTGACTGGTGGC TGCCTGAGGAATACCAGTGGGCAAGAGAATTAGCATTTCTGGAGCATCTGCTGTCTGAGC AGCCCCTGGGTGCGTCCACTTTCTGGGCACGTGAGGTTGGGCCTTGGCCGCCTGAGCCCT TGAGTTGGTCACTTGAACCTTGGGAATATTGAGATTATATTCTCCTGCCTTTTAAAAAGA TGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAACTGGACAGTGAAGATCTGGCCT CCCTCAAGTTCCTGAGCCTGGACTACATTCCGCAAAGGAAGCAAGAACCCATCAAGGATG CCTTGATGTTATTCCAGAGACTCCAGGAAAAGAGAATGTTGGAGGAAAGCAATCTGTCCT TCCTGAAGGAGCTGCTCTTCCGAATTAATAGACTGGATTTGCTGATTACCTACCTAAACA CTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCAGGCAGGGCTCANATTTCTGCCT ACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGCAGATCAGAAATGAGGTCCTTTA NGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTGGATGATGACATGAACCCTGCCT GGAATTTTTCATAGAGATTGAGAAAAAGGTCATTCTGGGGAAAAAGAAGTGGAACAACCT TAGAAAGATCTGCGCCCAATCAACAGAAGCCGGTGAATATACCCCCCCTTAAAATTTCCC AGGGGGGGGATTGGTGGGGAGCAACCCGACTTCCCAAACAGGAAGTATCCAACTTGTGAG GTTCA >OriGene 3' read for NM_033355 unedited
GGAGTGCACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTGGGT TTTTTTAAGATCAAATTATATTTTATTCTATTAAAAAATGTTTTTGAAAAAAGATACTTA AATTTTAAAGATAACTTAATTCCTAATGATTTAAAATAATCCAAGCAGAGATGAAAGAGC AAATGCAAATGCATAAAAAGACCCCAGAGCATTGTTAGCAAAAAGCAAATATAGTTAGCC AAGCATATATATATCATAAAAGCAATAAGAAGGCATAAAGCAAGTTTGGGGAGAGCTTAT TTAAAACTTGTAAAAATCATTTGAATTTTTAAAAGTTTTCAAACAAATTTTGTTTTATTA AAAAAAAAATTTTTTTTTGAGACGGGGTCTCGTTCTGTCACCCAAGCTTGAGTGCAGCGG TGTGAACATGGCTCACCGCAGCCTTGACCTCTTGGGTTCAAGCAATCCTCCCACCTCAAC CTCCCAAAGTGCTGGGACCATAGACATGAGCCACCATACGTGGCTACTTTCTAATTTTTT TAATGTGGTAGTGTGGAATTTAGCTTTGAAAGAGAATTAAAGTTAATAGAATATTAACAG TGGGCTTATAGTTGATAGATAACAATCTGAATCATAAGCCCTCTATATTCATAGCTGGAT TTTGTACGTGTCTGGTATTTATTGCCACCAGCTAAAAAACATCCCAAGAGCCTTAGCTTA ANAAAAAGGAAACGGGGTATTGTGGTAAATCCCATTAACCTTGCCTTATAACAGGCAAAC ACTTATTAGTGAAATCTTNAATATAATAAACTAGGTATTCAAAAAGCTCTGCCGGCGTGC TTCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_033355 |
| Insert Size | 4400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033355.2, NP_203519.1 |
| RefSeq Size | 2908 bp |
| RefSeq ORF | 1440 bp |
| Locus ID | 841 |
| UniProt ID | Q14790 |
| Domains | DED, CASc, ICE_p10, ICE_p20 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Alzheimer's disease, Apoptosis, Huntington's disease, NOD-like receptor signaling pathway, p53 signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Viral myocarditis |
| Gene Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (B) includes different segments in its 5' UTR and lacks a 5' coding region segment, compared to variant G. This results in translation at a downstream start codon, and the encoded protein (isoform B) has a shorter N-terminus when it is compared to isoform G. CCDS Note: This CCDS representation is based on AF422928.1 and U58143.1, and on data in PMID:11917123. This 479 aa isoform is also supported by homologous proteins, including pig NP_001026949.1, cow NP_001039435.1, mouse NP_033942.1, rat NP_071613.1 and chicken NP_989923.1. In addition, macaque AB172864.1 supports the full-length splice pattern. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216990 | CASP8 (Myc-DDK-tagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant B |
CNY 3,656.00 |
|
| RC216990L3 | Lenti-ORF clone of CASP8 (Myc-DDK-tagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant B |
CNY 6,650.00 |
|
| RC216990L4 | Lenti-ORF clone of CASP8 (mGFP-tagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant B |
CNY 6,650.00 |
|
| RG216990 | CASP8 (tGFP-tagged) - Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant B |
CNY 5,256.00 |
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