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GJA1 (NM_000165) Human Untagged Clone
CAT＃: SC125548
GJA1 (untagged)-Human gap junction protein, alpha 1, 43kDa (GJA1)
CNY 5,488.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AVSD3; CMDR; CX43; EKVP; EKVP3; GJAL; HLHS1; HSS; ODDD; PPKCA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC125548 sequence for NM_000165 edited (data generated by NextGen Sequencing)
ATGGGTGACTGGAGCGCCTTAGGCAAACTCCTTGACAAGGTTCAAGCCTACTCAACTGCT GGAGGGAAGGTGTGGCTGTCAGTACTTTTCATTTTCCGAATCCTGCTGCTGGGGACAGCG GTTGAGTCAGCCTGGGGAGATGAGCAGTCTGCCTTTCGTTGTAACACTCAGCAACCTGGT TGTGAAAATGTCTGCTATGACAAGTCTTTCCCAATCTCTCATGTGCGCTTCTGGGTCCTG CAGATCATATTTGTGTCTGTACCCACACTCTTGTACCTGGCTCATGTGTTCTATGTGATG CGAAAGGAAGAGAAACTGAACAAGAAAGAGGAAGAACTCAAGGTTGCCCAAACTGATGGT GTCAATGTGGACATGCACTTGAAGCAGATTGAGATAAAGAAGTTCAAGTACGGTATTGAA GAGCATGGTAAGGTGAAAATGCGAGGGGGGTTGCTGCGAACCTACATCATCAGTATCCTC TTCAAGTCTATCTTTGAGGTGGCCTTCTTGCTGATCCAGTGGTACATCTATGGATTCAGC TTGAGTGCTGTTTACACTTGCAAAAGAGATCCCTGCCCACATCAGGTGGACTGTTTCCTC TCTCGCCCCACGGAGAAAACCATCTTCATCATCTTCATGCTGGTGGTGTCCTTGGTGTCC CTGGCCTTGAATATCATTGAACTCTTCTATGTTTTCTTCAAGGGCGTTAAGGATCGGGTT AAGGGAAAGAGCGACCCTTACCATGCGACCAGTGGTGCGCTGAGCCCTGCCAAAGACTGT GGGTCTCAAAAATATGCTTATTTCAATGGCTGCTCCTCACCAACCGCTCCCCTCTCGCCT ATGTCTCCTCCTGGGTACAAGCTGGTTACTGGCGACAGAAACAATTCTTCTTGCCGCAAT TACAACAAGCAAGCAAGTGAGCAAAACTGGGCTAATTACAGTGCAGAACAAAATCGAATG GGGCAGGCGGGAAGCACCATCTCTAACTCCCATGCACAGCCTTTTGATTTCCCCGATGAT AACCAGAATTCTAAAAAACTAGCTGCTGGACATGAATTACAGCCACTAGCCATTGTGGAC CAGCGACCTTCAAGCAGAGCCAGCAGTCGTGCCAGCAGCAGACCTCGGCCTGATGACCTG GAGATCTAG Clone variation with respect to NM_000165.3 >OriGene 5' read for NM_000165 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGC ACGAGTATCAGCACTTTTCTTTCATTAGGGGGAAGGCGTGAGGAAAGTACCAAACAGCAG CGGAGTTTTAAACTTTAAATAGACAGGTCTGAGTGCCTGAACTTGCCTTTTCATTTTACT TCATCCTCCAAGGAGTTCAATCACTTGGCGTGACTTCACTACTTTTAAGCAAAAGAGTGG TGCCCAGGCAACATGGGTGACTGGAGCGCCTTAGGCAAACTCCTTGACAAGGTTCAAGCC TACTCAACTGCTGGAGGGAAGGTGTGGCTGTCAGTACTTTTCATTTTCCGAATCCTGCTG CTGGGGACAGCGGTTGAGTCAGCCTGGGGAGATGAGCAGTCTGCCTTTCGTTGTAACACT CAGCAACCTGGTTGTGAAAATGTCTGCTATGACAAGTCTTTCCCAATCTCTCATGTGCGC TTCTGGGTCCTGCAGATCATATTTGTGTCTGTACCCACACTCTTGTACCTGGCTCATGTG TTCTATGTGATGCGAAAGGAAGAGAAACTGAACAAGAAAGAGGAAGAACTCAAGGTTGCC CAACTGATGGTGTCAATGTGGACATGCACTTGAAGCAGATCGAATAAAAGAAGTTCAAGT ACGGTATTGAAGAGCATGGNTAGGTGAAATGCGAGGGGGGGGGTTGCTGCNAACCTACAT CATCAGTATCCTCTTCAGTCATTTTTTGAGGGTGCCTCTTGCTGATNCCAGTGGACATCT ATGGATTCNCTTGGGAGGGCTGGTTACACTGCAAAAAAGACCCTGCACATCAGGGGGGGC CGTGTTCCTCTCTCGCCCACGGAGAAACATCTCATATTCTGCCGGGGGGGGGGCNCCGTG GGGGCCCTGGCCTGAAATAAATGACTCTTTTTGTTTTT >OriGene 3' read for NM_000165 unedited
GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTATACTAAATTAAAACTT TATTGAATAAAGAACACTCTCCAGAACACATGATCTGATGGACTAGGTCTACATTACATG CAATGAAGCTGAACATGACCGTAGTTTAACATGGAATGTCAAACAAATTAGTAATTTTCA TTGTACAAAACTGTTTATGTAGCTGACATGCATGCAAGAAGAAAAGTGTCATACTCAGCA TTTTCACCAATCTTCTGTGATGAGTTTTAAAAAAGGCATTTGGAGAAACTGGTAGATGTC TTCAACATATAGTTCAAATAAATTAGTTGTATGTGTACTACTGAATCCTCTTTCAACCTC TCTTGCATTCCAAATAAAATCTTAGTGCAAACATCAAAGTTGCTGGTCACTCCAAAAGAC TGCTAAACTCATAATAGAATACAAGTTCTGAATTACTGTGTATTAAGTAGGAAAACCCAA GAATACCAGTTACTGCAAAATTCATTTAGGCACATTTAATAGTCCACTCTAAGCATTTTT TCTAAGTCTCTCACATTAATGACTAAAGCCTGTTTACAGTACTAAAATTCTATCATTCAA GCATCAATGATTACATTTCAGAAAAAGGAAATTCATTTCAGTAATAGCATTACTGCTATA TCCTANAAAAAGGAGAGAAAATAAAGAATCATAATTAACAAAACCAGTATCCATAATAGG ATACACAGGGGAAAAACATTNTAGGAGAAANAAAAAGTGTGCTTTACTTGCCCAGCANGA ATATATAATGTAAACACATATTGGCCACACACAAAAATCTGAGAAGGTTNCAATTTGACC CATAGTGCATATTTTCAAACTGNGTTGNGAGTTTACTGATCTGGCTGACATACTGACAAC AAACAACTCTCAACACTATATGATGGTATATAGCTGGCTGTTCCTGACTTACTAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000165 |
| Insert Size | 2940 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000165.2, NP_000156.1 |
| RefSeq Size | 3088 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 2697 |
| UniProt ID | P17302 |
| Domains | CNX, Connexin43 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Gap junction |
| Gene Summary | This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Endometrial Stromal Decidualization Responds Reversibly to Hormone Stimulation and Withdrawal
,Yu, J;Berga, SL;Johnston-MacAnanny, E;Sidell, N;Bagchi, IC;Bagchi, MK;Taylor, RN;,
Endocrinology
,PubMed ID 27035651
[GJA1]
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Tumor-induced loss of mural Connexin 43 gap junction activity promotes endothelial proliferation
,Choudhary, M;Naczki, C;Chen, W;Barlow, KD;Case, LD;Metheny-Barlow, LJ;,
BMC Cancer
,PubMed ID 26002762
[GJA1]
|
|
Exome Sequencing Reveals Mutation in GJA1 as a Cause of Keratoderma-Hypotrichosis-Leukonychia Totalis Syndrome
,Wang, H;Cao, X;Lin, Z;Lee, M;Jia, X;Ren, Y;Dai, L;Guan, L;Zhang, J;Lin, X;Zhang, J;Chen, Q;Feng, C;Zhou, EY;Yin, J;Xu, G;Yang, Y;,
Hum. Mol. Genet.
,PubMed ID 25168385
[GJA1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205303 | GJA1 (Myc-DDK-tagged)-Human gap junction protein, alpha 1, 43kDa (GJA1) |
CNY 5,488.00 |
|
| RC205303L1 | Lenti ORF clone of Human gap junction protein, alpha 1, 43kDa (GJA1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205303L2 | Lenti ORF clone of Human gap junction protein, alpha 1, 43kDa (GJA1), mGFP tagged |
CNY 7,888.00 |
|
| RC205303L3 | Lenti ORF clone of Human gap junction protein, alpha 1, 43kDa (GJA1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205303L4 | Lenti ORF clone of Human gap junction protein, alpha 1, 43kDa (GJA1), mGFP tagged |
CNY 7,888.00 |
|
| RG205303 | GJA1 (tGFP-tagged) - Human gap junction protein, alpha 1, 43kDa (GJA1) |
CNY 7,088.00 |
