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TIE1 (NM_005424) Human Untagged Clone
CAT＃: SC125505
TIE1 (untagged)-Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1)
CNY 7,928.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | JTK14; LMPHM11; TIE |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_005424 edited
CTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATT CGGCACGAGGAGCATCTGACCCCAGGCCCAGCTCGTCCTGGCTGGCCTGGGTCGGCCTCT GGAGTATGGTCTGGCGGGTGCCCCCTTTCTTGCTCCCCATCCTCTTCTTGGCTTCTCATG TGGGCGCGGCGGTGGACCTGACGCTGCTGGCCAACCTGCGGCTCACGGACCCCCAGCGCT TCTTCCTGACTTGCGTGTCTGGGGAGGCCGGGGCGGGGAGGGGCTCGGACGCCTGGGGCC CGCCCCTGCTGCTGGAGAAGGACGACCGTATCGTGCGCACCCCGCCCGGGCCACCCCTGC GCCTGGCGCGCAACGGTTCGCACCAGGTCACGCTTCGCGGCTTCTCCAAGCCCTCGGACC TCGTGGGCGTCTTCTCCTGCGTGGGCGGTGCTGGGGCGCGGCGCACGCGCGTCATCTACG TGCACAACAGCCCTGGAGCCCACCTGCTTCCAGACAAGGTCACACACACTGTGAACAAAG GTGACACCGCTGTACTTTCTGCACGTGTGCACAAGGAGAAGCAGACAGACGTGATCTGGA AGAGCAACGGATCCTACTTCTACACCCTGGACTGGCATGAAGCCCAGGATGGGCGGTTCC TGCTGCAGCTCCCAAATGTGCAGCCACCATCGAGCGGCATCTACAGTGCCACTTACCTGG AAGCCAGCCCCCTGGGCAGCGCCTTCTTTCGGCTCATCGTGCGGGGTTGTGGGGCTGGGC GCTGGGGGCCAGGCTGTACCAAGGAGTGCCCAGGTTGCCTACATGGAGGTGTCTGCCACG ACCATGACGGCGAATGTGTATGCCCCCCTGGCTTCACTGGCACCCGCTGTGAACAGGCCT GCAGAGAGGGCCGTTTTGGGCAGAGCTGCCAGGAGCAGTGCCCAGGCATATCAGGCTGCC GGGGCCTCACCTTCTGCCTCCCAGACCCCTATGGCTGCTCTTGTGGATCTGGCTGGAGAG GAAGCCAGTGCCAAGAAGCTTGTGCCCCTGGTCATTTTGGGGCTGATTGCCGACTCCAGT GCCAGTGTCAGAATGGTGGCACTTGTGACCGGTTCAGTGGTTGTGTCTGCCCCTCTGGGT GGCATGGAGTGCACTGTGAGAAGTCAGACCGGATCCCCCAGATCCTCAACATGGCCTCAG AACTGGAGTTCAACTTAGAGACGATGCCCCGGATCAACTGTGCAGCTGCAGGGAACCCCT TCCCCGTGCGGGGCAGCATAGAGCTACGCAAGCCAGACGGCACTGTGCTCCTGTCCACCA AGGCCATTGTGGAGCCAGAGAAGACCACAGCTGAGTTCGAGGTGCCCCGCTTGGTTCTTG CGGACAGTGGGTTCTGGGAGTGCCGTGTGTCCACATCTGGCGGCCAAGACAGCCGGCGCT TCAAGGTCAATGTGAAAGTGCCCCCCGTGCCCCTGGCTGCACCTCGGCTCCTGACCAAGC AGAGCCGCCAGCTTGTGGTCTCCCCGCTGGTCTCGTTCTCTGGGGATGGACCCATCTCCA CTGTCCGCCTGCACTACCGGCCCCAGGACAGTACCATGGACTGGTCGACCATTGTGGTGG ACCCCAGTGAGAACGTGACGTTAATGAACCTGAGGCCAAAGACAGGATACAGTGTTCGTG TGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAGGGGGCCTGGGGGCCTCCCACCCTCATGA CCACAGACTGTCCTGAGCCTTTGTTGCAGCCGTGGTTGGAGGGCTGGCATGTGGAAGGCA CTGACCGGCTGCGAGTGAGCTGGTCCTTGCCCTTGGTGCCCGGGCCACTGGTGGGCGACG GTTTCCTGCTGCGCCTGTGGGACGGGACACGGGGGCAGGAGCGGCGGGAGAACGTCTCAT CCCCCCAGGCCCGCACTGCCCTCCTGACGGGACTCACGCCTGGCACCCACTACCAGCTGG ATGTGCAGCTCTACCACTGCACCCTCCTGGGCCCGGCCTCGCCCCCTGCACACGTGCTTC TGCCCCCCAGTGGGCCTCCAGCCCCCCGACACCTCCACGCCCAGGCCCTCTCAGACTCCG AGATCCAGCTGACATGGAAGCACCCGGAGGCTCTGCCTGGGCCAATATCCAAGTACGTTG TGGAGGTGCAGGTGGCTGGGGGTGCAGGAGACCCACTGTGGATAGACGTGGACAGGCCTG AGGAGACAAGCACCATCATCCGTGGCCTCAACGCCAGCACGCGCTACCTCTTCCGCATGC GGGCCAGCATTCAGGGGCTCGGGGACTGGAGCAACACAGTAGAAGAGTCCACCCTGGGCA ACGGGCTGCAGGCTGAGGGCCCAGTCCAAGAGAGCCGGGCAGCTGAAGAGGGCCTGGATC AGCAGCTGATCCTGGCGGTGGTGGGCTCCGTGTCTGCCACCTGCCTCACCATCCTGGCCG CCCTTTTAACCCTGGTGTGCATCCGCAGAAGCTGCCTGCATCGGAGACGCACCTTCACCT ACCAGTCAGGCTCGGGCGAGGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGACACTTA CCCGGCGGCCAAAACTGCAGCCCGAGCCCCTGAGCTACCCAGTGCTAGAGTGGGAGGACA TCACCTTTGAGGACCTCATCGGGGAGGGGAACTTCGGCCAGGTCATCCGGGCCATGATCA AGAAGGACGGGCTGAAGATGAACGCAGCCATCAAAATGCTGAAAGAGTATGCCTCTGAAA ATGACCATCGTGACTTTGCGGGAGAACTGGAAGTTCTGTGCAAATTGGGGCATCACCCCA ACATCATCAACCTCCTGGGGGCCTGTAAGAACCGAGGTTACTTGTATATCGCTATTGAAT ATGCCCCCTACGGGAACCTGCTAGATTTTCTGCGGAAAAGCCGGGTCCTAGAGACTGACC CAGCTTTTGCTCGAGAGCATGGGACAGCCTCTACCCTTAGCTCCCGGCAGCTGCTGCGTT TCGCCAGTGATGCGGCCAATGGCATGCAGTACCTGAGTGAGAAGCAGTTCATCCACAGGG ACCTGGCTGCCCGGAATGTGCTGGTCGGAGAGAACCTAGCCTCCAAGATTGCAGACTTCG GCCTTTCTCGGGGAGAGGAGGTTTATGTGAAGAAGACGATGGGGCGTCTCCCTGTGCGCT GGATGGCCATTGAGTCCCTGAACTACAGTGTCTATACCACCAAGAGTGATGTCTGGTCCT TTGGAGTCCTTCTTTGGGAGATAGTGAGCCTTGGAGGTACACCCTACTGTGGCATGACCT GTGCCGAGCTCTATGAAAAGCTGCCCCAGGGCTACCGCATGGAGCAGCCTCGAAACTGTG ACGATGAAGTGTACGAGCTGATGCGTCAGTGCTGGCGGGACCGTCCCTATGAGCGACCCC CCTTTGCCCAGATTGCGCTACAGCTAGGCCGCATGCTGGAAGCCAGGAAGGCCTATGTGA ACATGTCGCTGTTTGAGAACTTCACTTACGCGGGCATTGATGCCACAGCTGAGGAGGCCT GAGCTGCCATCCAGCCAGAACGTGGCTCTGCTGGCCGGAGCAAACTCTGCTGTCTAACCT GTGACCAGTCTGACCCTTACAGCCTCTGACTTAAGCTGCCTCAAGGAATTTTTTTAACTT AAGGGAGAAAAAAAGGGATCTGGGGATGGGGTGGGCTTAGGGGAACTGGGTTCCCATGCT TTGTAGGTGTCTCATAGCTATCCTGGGCATCCTTCTTTCTAGTTCAGCTGCCCCACAGGT GTGTTTCCCATCCCACTGCTCCCCCAACACAAACCCCCACTCCAGCTCCTTCGCTTAAGC CAGCACTCACACCACTAACATGCCCTGTTCAGCTACTCCCA >OriGene 5' read for NM_005424 unedited
GAAATTACGCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA GCATATCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG CGAATTCGGCACGAGGAGCATCTGACCCCAGGCCCAGCTCGTCCTGGCTGGCCTGGGTCG GCCTCTGGAGTATGGTCTGGCGGGTGCCCCCTTTCTTGCTCCCCATCCTCTTCTTGGCTT CTCATGTGGGCGCGGCGGTGGACCTGACGCTGCTGGCCAACCTGCGGCTCACGGACCCCC AGCGCTTCTTCCTGACTTGCGTGTCTGGGGAGGCCGGGGCGGGGAGGGGCTCGGACGCCT GGGGCCCGCCCCTGCTGCTGGAGAAGGACGACCGTATCGTGCGCACCCCGCCCGGGCCAC CCCTGCGCCTGGCGCGCAACGGTTCGCACCAGGTCACGCTTCGCGGCTTCTCCAAGCCCT CGGACCTCGTGGGCGTCTTCTCCTGCGTGGGCGGTGCTGGGGCGCGGCGCACGCGCGTCA TCTACGTGCACAACAGCCCTGGAGCCCACCTGCTTCCAGACAAGGTCACACACACTGTGA ACAAAGGTGACACCGCTGTACTTTCTGCACGTGTGCACAAGGAGAAGCAGACAGACGTGA TCTGGAAGAGCAACGGATCCTACTTCTACACCCTGGACTGGCATGAAGCCCAGGATGGGG CGGTTCCTGCTGCAGCTCCCAAATGTGCAGCCACCATCGAGCGGCATCTACAGTGCCACT TACCTGGAAGCCAGCCCCCTGGGCAGCGCCTTCTTTCGGCTCATCGTGCGGGGNTTGTGG GGCTTGGCGCTGGNGGCCCAGCTGTACCAAGGATGCCCCAGGTGCCTACTGGNAGGTGTC TGCCACGACATGACG >OriGene 3' read for NM_005424 unedited
AATGGGGGGNACATTCATTNATTCTGATGACAGGCCGGGCGTGGGAGTAGCTGAACAGGG CATGTTAGTGGTGTGAGTGCTGGCTTAAGCGAAGGAGCTGGAGTGGGGGTTTGTGTTGGG GGAGCAGTGGGATGGGAAACACACCTGTGGGGCAGCTGAACTAGAAAGAAGGATGCCCAG GATAGCTATGAGACACCTACAAAGCATGGGAACTCATTTTCCCTAAGCCCACCCCATCCC CAGATCCCTTTTTTTCTCCCTTAAGTTAAAAAAATTCCTTGAGGCAGCTTAAGTCAGAGG CTGTAAGGGTCAGACTGGTCACAGGTTAGACAGCAGAGTTTGCTCCGGCCAGCAGAGCCA CGTTCTGGCTGGATGGCAGCTCAGGCCTCCTCAGCTGTGGCATCAATGCCCGCGTAAGTG AAGTTCTCAAACAGCGACATGTTCACATAGGCCTTCCTGGCTTCCAGCATGCGGCCTAGC TGTAGCGCAATCTGGGCAAAGGGGGGTCGCTCATAGGGACGGTCCCGCCAGCACTGACGC ATCAGCTCGTACACTTCATCGTCACAGTTTCGAGGCTGCTCCATGCGGTAGCCCTGGGGC AGCTTTTCATAGAGCTCGGCACAGGTCATGCCACAGTAGGGTGTACCTCCAAGGCTCACT ATCTCCCAAAGAAGGACTTCCAAAGGACCAGACATCACTCTTTGTGGGTATAGACACTGT AGTTCAGGGACTCAATGGCCATCCAGCGCACAGGGAGACGCCCCATCGTCTTTTTCACAT AAACCTCCTCTCCCCGAGAAAGCCGGAATCTTGAATCTTGGAAGCTAGGTTCTTTCCGAC CAGACTTTCCGGCAGGCAGGTCCCTGTGGGAGAACTGGTTCTAACTCAGGACCTGCTGCA CTTGCCCGCTTCCTA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005424 |
| Insert Size | 4200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005424.2, NP_005415.1 |
| RefSeq Size | 4000 bp |
| RefSeq ORF | 3417 bp |
| Locus ID | 7075 |
| UniProt ID | P35590 |
| Domains | pkinase, TyrKc, S_TKc, ig, IG, FN3, EGF, EGF |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Gene Summary | This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207771 | TIE1 (Myc-DDK-tagged)-Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1) |
CNY 7,928.00 |
|
| RC207771L1 | Lenti ORF clone of Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), Myc-DDK-tagged |
CNY 10,328.00 |
|
| RC207771L2 | Lenti ORF clone of Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), mGFP tagged |
CNY 13,210.00 |
|
| RC207771L3 | Lenti ORF clone of Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), Myc-DDK-tagged |
CNY 10,328.00 |
|
| RC207771L4 | Lenti ORF clone of Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), mGFP tagged |
CNY 13,210.00 |
|
| RC600056 | TIE1 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens tyrosine kinase with immunoglobulin-like and EGF-like domains 1, transcript variant 1, Signal peptide (1-21) plus EC domain (22-759) |
CNY 5,560.00 |
|
| RG207771 | TIE1 (tGFP-tagged) - Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1) |
CNY 9,528.00 |
|
| SC323581 | TIE1 (untagged)-Kinase deficient mutant (K870M) of Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1) |
CNY 7,928.00 |
