ASMT (BC001620) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Acetylserotonin methyltransferase (Y chromosome); acetylserotonin N-methyltransferase; acetylserotonin O-methyltransferase; ASMTY; HIOMT; HIOMTY; OTTHUMP00000022834
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for BC001620 edited ATGGGATCCTCAGAGGACCAGGCCTATCGCCTCCTTAATGACTACGCCAACGGCTTCATG GTGTCCCAGGTTCTCTTCGCCGCCTGCGAGCTGGGCGTGTTTGACCTTCTCGCCGAGGCC CCAGGGCCCCTGGACGTGGCGGCAGTGGCTGCAGGTGTGAGGGCCAGCGCCCATGGGACA GAGCTCCTGCTGGACATCTGTGTGTCCCTGAAGCTGCTGAAAGTGGAGACGAGGGGAGGA AAAGCTTTCTATCGAAACACAGAGCTGTCCAGCGACTACCTGACCACGGTCAGCCCGACG TCACAATGCAGCATGCTGAAGTACATGGGCAGGACCAGCTACCGGTGCTGGGGCCACCTG GCAGACGCCGTGAGAGAAGGAAGGAACCAGTACCTGGAGACGTTTGGCGTTCCCGCTGAA GAGCTTTTTACGGCCATCTACAGGTCCGAGGGCGAGCGGCTACAGTTCATGCAAGCTCTG CAGGAGGTCTGGAGCGTCAACGGGAGAAGCGTGCTGACCGCCTTTGACCTGTCAGTGTTC CCACTTATGTGTGACCTTGGTGGGGATTTCTTCAAAGACCCTCTTCCGGAAGCTGATCTG TACATCCTGGCCAGGGTCCTCCATGACTGGGCAGATGGAAAGTGCTCACACCTGCTGGAG AGGATCTACCACACTTGCAAGCCAGGTGGTGGCATTCTGGTAATTGAAAGCCTCCTGGAT GAAGACAGGCGAGGTCCTCTGCTCACGCAGCTCTACTCTCTGAACATGCTTGTGCAGACG GAAGGGCAGGAGAGGACCCCCACCCACTACCACATGCTCCTCTCTTCTGCTGGCTTCAGA GACTTCCAGTTTAAGAAAACAGGAGCCATTTATGATGCCATTTTAGCCAGGAAATAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001620 unedited GATCAGGATATTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGCACGAGGAAGCCC GGCTGGATTGGAGACAAGATGGGATCCTCAGAGGACCAGGCCTATCGCCTCCTTAATGAC TACGCCAACGGCTTCATGGTGTCCCAGGTTCTCTTCGCCGCCTGCGAGCTGGGCGTGTTT GACCTTCTCGCCGAGGCCCCAGGGCCCCTGGACGTGGCGGCAGTGGCTGCAGGTGTGAGG GCCAGCGCCCATGGGACAGAGCTCCTGCTGGACATCTGTGTGTCCCTGAAGCTGCTGAAA GTGGAGACGAGGGGAGGAAAAGCTTTCTATCGAAACACAGAGCTGTCCAGCGACTACCTG ACCACGGTCAGCCCGACGTCACAATGCAGCATGCTGAAGTACATGGGCAGGACCAGCTAC CGGTGCTGGGGCCACCTGGCAGACGCCGTGAGAGAAGGAAGGAACCAGTACCTGGAGACG TTTGGCGTTCCCGCTGAAGAGCTTTTTACGGCCATCTACAGGTCCGAGGGCGAGCGGCTA CAGTTCATGCAAGCTCTGCANGAGGTCTGGAGCGTCAACGGGAGAAGCGTGCTGACCGCC TTTGACCTGTCAGTGTTCCCACTTATGTGTGACCTTGGTGGGGATTTCTTCANAGACCCT CTTCCGGAAGCTGATCTGTACATCCTGGCCAGGGTCCTCCATGACTGGGCAGATGGAAAG TGCTCACACCTGCTTGAGAGGATCTACCACACTTGCAAGCCAGGTGGGGGCATTCTGGTA TTGAAAGCCTCCTGGATGAAGACAGGCGAGGTCCTTTGCTCACGCAGCTTTACTCTCTGA AAATGCTTGTGCAACGGAAAGGGCAGAAAGGACCCCCACCACTACCAATGCTCCTCTTTC TGCTGGGTTAGAAACTTCCAT
Restriction Sites	Please inquire
ACCN	BC001620
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001620.1, AAH01620.1
RefSeq Size	1026 bp
Locus ID	438
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Tryptophan metabolism
Gene Summary	This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2010]

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