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ID1 (NM_002165) Human Untagged Clone
CAT＃: SC125462
ID1 (untagged)-Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), transcript variant 1
CNY 1,800.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHb24; ID |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_002165, the custom clone sequence may differ by one or more nucleotides
ATGAAAGTCGCCAGTGGCAGCACCGCCACCGCCGCCGCGGGCCCCAGCTGCGCGCTGAAGGCCGGCAAGA CAGCGAGCGGTGCGGGCGAGGTGGTGCGCTGTCTGTCTGAGCAGAGCGTGGCCATCTCGCGCTGCGCCGG GGGCGCCGGGGCGCGCCTGCCTGCCCTGCTGGACGAGCAGCAGGTAAACGTGCTGCTCTACGACATGAAC GGCTGTTACTCACGCCTCAAGGAGCTGGTGCCCACCCTGCCCCAGAACCGCAAGGTGAGCAAGGTGGAGA TTCTCCAGCACGTCATCGACTACATCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTGGAAC CCCCGGGGGCCGAGGGCTGCCGGTCCGGGCTCCGCTCAGCACCCTCAACGGCGAGATCAGCGCCCTGACG GCCGAGGCGGCATGCGTTCCTGCGGACGATCGCATCTTGTGTCGCTGA >OriGene 5' read for NM_002165 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTTCCGTATCTGCTTCGGG CTTCCACCTCATTTTTTTCGCTTTGCCCATTCTGTTTCAGCCAGTCGCCAAGAATCATGA AAGTCGCCAGTGGCAGCACCGCCACCGCCGCCGCGGGCCCCAGCTGCGCGCTGAAGGCCG GCAAGACAGCGAGCGGTGCGGGCGAGGTGGTGCGCTGTCTGTCTGAGCAGAGCGTGGCCA TCTCGCGCTGCGCCGGGGGCGCCGGGGCGCGCCTGCCTGCCCTGCTGGACGAGCAGCAGG TAAACGTGCTGCTCTACGACATGAACGGCTGTTACTCACGCCTCAAGGAGCTGGTGCCCA CCCTGCCCCAGAACCGCAAGGTGAGCAAGGTGGAGATTCTCCAGCACGTCATCGACTACA TCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTGGGACCCCCGGGGGCCGAG GGCTGCCGGTCCGGGCTCCGCTCAGCACCCTCAACGGCGAGATCAGCGCCCTGACGGCCG AGGCGGCATGCGTTCCTGCGGACGATCGCATCTTGTGTCGCTGAAGCGCCTCCCCCAGGG ACCGGCGGACCCCAGCCATCCAGGGGGCAAGAGGAATTACGTGCTCTGTGGGTCTCCCCC AACGCGCCTCGCCGGATCTGAGGGAGAACAAGACCGATCGGCGGCCACTGCGCCCTTAAC TGCATCCAGCCTGGGGCTGAGGCTGANGCACTGGCGAGGAGAGGGCGCTCCTCTCTGCAC ACCTACTAGTCACCAGAGACTTTGGGNGGTGGGATTTCACTCGTGTGTTTTCTATTTTTG AAAAGCAGACATTTTAAAAATGCTCACGTTTGGTGGCTCTCANATTCTGAGAAANTGCTT TGTATGTATATACATGATCACGACTGAGATATGTTTTACATAGTCTGTGGGCA >OriGene 3' read for NM_002165 unedited
CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAACCATCTATTTTATTTG TTTAATAACAAAAAAACAGCCCCACAAAACTATTGTAAAACAATATTCTCAGTCGGGGAT CATTGTAATATACAATACAAAGCAATTTCCTCAAAAATCTGAGAAGCACCAAACGTGACC ATTTTTTAAAATGTCTGCTTTTCAAAAAATAAAAACACACGAGTGGAATCCCACCCCCTA AAGTCTCTGGTGACTAGTAGGGGTGCAAAGAGGAGCGCCCTCTCCTCGCCAGTGCCTCAG CCTCAGCCCCAGGCTGGATGCAGTTAAGGGCGCAGTGGCCGCCGATCGGTCTTGTTCTCC CTCAAATCCGGCGAGGCGCGTTGGGGGAGACCCACAGAGCACGTAATTCCTCTTGCCCCC TGGATGGCTGGGGTCCGCCGGTCCCTGGGGGAGGCGCTTCAGCGACACAAGATGCGATCG TCCGCAGGAACGCATGCCGCCTCGGCCGTCAGGGCGCTGATCTCGCCGTTGAGGGTGCTG AGCGGAGCCCGGACCGGCAGCCCTCGGCCCCCGGGGGTCCCAACTTCGGATTCCGAGTTC AGCTCCAACTGAAGGTCCCTGATGTACTCGATGACGTGCTGGAGAATCTCCACCTTGCTC ACCTTGCCGTTCTGGGGCAGGGTGGGCACCAGCTCCTTGAGGCGTTGAGAACAGCCGTTC ATGTCGTAAAGCAGCACGTTTACCTGCTGCTCGCCCACAGGAAGGCCAGGCCCCCCCCGC GCCCCCGCGCACCGCGAAATGCCCCCTCTCTGCTACACGTAAGTGCACCACCTCCCCCGA ACGTTCCGTGTTTGGCGGCCTTCACGCCCAATTGGGGCCCGCGGGGCGGGGCGGGCTGCT ACTGCCACTTAATGATTCTGGCCTTGGCCGAACCAATGGCCACCCAAAAAGTAGGGAACC CCGAGCAAATGGAACCTCGGCCGATTGCGCCCTCCTATTGAGTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002165 |
| Insert Size | 1060 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002165.2, NP_002156.2 |
| RefSeq Size | 993 bp |
| RefSeq ORF | 468 bp |
| Locus ID | 3397 |
| UniProt ID | P41134 |
| Domains | HLH |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | TGF-beta signaling pathway |
| Gene Summary | The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longer isoform (a, also called ID-1H). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Role of ID Proteins in BMP4 Inhibition of Profibrotic Effects of TGF-β2 in Human TM Cells
,Mody, AA;Wordinger, RJ;Clark, AF;,
Invest. Ophthalmol. Vis. Sci
,PubMed ID 28159972
[ID1]
|
|
Contribution of Xanthine Oxidoreductase to Mammary Epithelial and Breast Cancer Cell Differentiation In Part Modulates Inhibitor of Differentiation-1
,Mehdi A. Fini, Jenifer Monks, Susan M. Farabaugh, and Richard M. Wright,
Mol. Cancer Res., Sep 2011; 9: 1242 - 1254.
[ID1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202061 | ID1 (Myc-DDK-tagged)-Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), transcript variant 1 |
CNY 1,800.00 |
|
| RC202061L1 | Lenti ORF clone of Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), transcript variant 1, Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC202061L2 | Lenti ORF clone of Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), transcript variant 1, mGFP tagged |
CNY 4,200.00 |
|
| RC202061L3 | Lenti ORF clone of Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), transcript variant 1, Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC202061L4 | Lenti ORF clone of Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), transcript variant 1, mGFP tagged |
CNY 4,200.00 |
|
| RG202061 | ID1 (tGFP-tagged) - Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), transcript variant 1 |
CNY 3,400.00 |
