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DNA Primase (PRIM2) (NM_000947) Human Untagged Clone
CAT＃: SC125450
PRIM2 (untagged)-Human primase, DNA, polypeptide 2 (58kDa) (PRIM2)
CNY 3,800.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | p58; PRIM2A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC125450 sequence for NM_000947 edited (data generated by NextGen Sequencing)
ATGGAGTTTTCTGGAAGAAAGTGGAGGAAGCTGAGGTTGGCAGGTGACCAGAGGAATGCT TCCTACCCTCATTGCCTTCAGTTTTACTTGCAGCCACCTTCTGAAAACATATCTTTAATA GAATTTGAAAACTTGGCTATTGATAGAGTTAAATTGTTAAAATCAGTTGAAAATCTTGGA GTGAGCTATGTGAAAGGAACTGAACAATACCAGAGTAAGTTGGAGAGTGAGCTTCGGAAG CTCAAGTTTTCCTACAGAGAAAACTTAGAAGATGAATATGAACCACGAAGAAGAGATCAT ATTTCTCATTTTATTTTGCGGCTTGCTTATTGCCAGTCTGAAGAACTTAGACGCTGGTTC ATTCAACAAGAAATGGATCTCCTTCGATTTAGATTTAGTATTTTACCCAAGGATAAAATT CAGGATTTCTTAAAGGATAGCCAATTGCAGTTTGAGGCTATAAGTGATGAAGAGAAGACT CTTCGAGAACAGGAGATTGTTGCCTCATCACCAAGTTTAAGTGGACTTAAGTTGGGGTTC GAGTCCATTTATAAGATCCCTTTTGCTGATGCTCTGGATTTGTTTCGAGGAAGGAAAGTC TATTTGGAAGATGGCTTTGCTTACGTACCACTTAAGGACATTGTGGCAATCATCCTGAAT GAATTTAGAGCCAAACTGTCCAAGGCTTTGGCATTAACAGCCAGGTCCTTGCCTGCTGTG CAGTCTGATGAAAGACTTCAGCCTCTGCTCAATCACCTCAGTCATTCCTACACTGGCCAA GATTACAGTACCCAGGGAAATGTTGGGAAGATTTCTTTAGATCAGATTGATTTGCTTTCT ACCAAATCCTTCCCACCTTGCATGCGTCAGTTACATAAAGCCTTGCGGGAAAATCACCAT CTTCGTCATGGAGGCCGAATGCAGTATGGCCTATTTCTGAAGGGCATTGGTTTAACTTTG GAACAGGCATTGCAGTTCTGGAAGCAAGAATTTATCAAAGGAAAGATGGATCCAGACAAG TTTGATAAAGGTTACTCTTACAACATCCGTCACAGCTTTGGAAAGGAAGGCAAGAGGACA GACTATACACCTTTCAGTTGCCTGAAGATTATTCTGTCCAATCCACCAAGCCAAGGGGAT TATCATGGGTGCCCATTCCGTCACAGTGATCCAGAGCTGCTGAAGCAAAAGTTGCAGTCA TACAAGATCTCTCCTGGAGGGATAAGCCAGATTTTGGATTTAGTAAAGGGGACACATTAC CAGGTAGCCTGTCAAAAATACTTTGAGATGATACACAATGTGGATGATTGTGGCTTTTCT TTGAATCATCCTAATCAGTTCTTTTGTGAGAGCCAACGTATTCTAAATGGTGGTAAAGAC ATAAAGAAGGAACCTATCCAACCAGAAACTCCTCAACCCAAACCAAGTGTCCAGAAAACC AAGGATGCATCATCTGCTCTGGCCTCTTTAAATTCCTCTCTGGAAATGGATATGGAAGGA CTAGAAGATTACTTTAGTGAAGATTCTTAG Clone variation with respect to NM_000947.2 >OriGene 5' read for NM_000947 unedited
GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAACAGTGGC TGCCACCGTTTGTGTTTTCCCGAGTTTGAATTCTTGCAGGTGACCAAGATGGAGTTTTCT GGAAGAAAGTGGAGGAAGCTGAGGTTGGCAGGTGACCAGAGGAATGCTTCCTACCCTCAT TGCCTTCAGTTTTACTTGCAGCCACCTTCTGAAAACATATCTTTAATAGAATTTGAAAAC TTGGCTATTGATAGAGTTAAATTGTTAAAATCAGTTGAAAATCTTGGAGTGAGCTATGTG AAAGGAACTGAACAATACCAGAGTAAGTTGGAGAGTGAGCTTCGGAAGCTCAAGTTTTCC TACAGAGAAAACTTAGAAGATGAATATGAACCACGAAGAAGAGATCATATTTCTCATTTT ATTTTGCGGCTTGCTTATTGCCAGTCTGAAGAACTTAGACGCTGGTTCATTCAACAAGAA ATGGATCTCCTTCGATTTAGATTTAGTATTTTACCCAAGGATAAAATTCAGGATTTCTTA AAGGATAGCCAATTGCAGTTTGAGGCTATAAGTGATGAAGAGAAGACTCTTCGAGAACAG GAGATTGTTGCCTCATCACCAAGTTTAAGTGGACTTAAGTTGGGGTTCGAGTCCATTTAT AAGATCCCTTTTGCTGATGCTCTGGATTTGTTTCGAGGAAGGAAAGTCTATTTGGAAGAT GGCTTTGCTTACGTACCACTTAAGGACATTGTGGGCATCATCCTGAATGAATNTAGAGCC AAACTGTCCAAGGGCTTTGCATTAACAGCCCAGTCCTTGCCTGCTGTGCAGTCTGATGAA AGACTCAGCCTCTGCTAATCACCCAGTCATCCTACCTGGCCCAGATACAGACCCAGGAAT >OriGene 3' read for NM_000947 unedited
CGGCGATGAGCCTACCCCCCCCTCACACACCCCGNGGTGGGGGTTNTAAAACAAATGTCC ANAAAGATGAGTCAAATACAAAGGATAANATTAAAACAAAGTTGTTCTTTATTGTAGTTT CTGGGGGACTTTAACCAGTCCCCAAAGCTGTTTTTATCTCCTAAAATAGGACTTAAATGA CATCACTCAAGTGACAGATTTTAATAGAAACTCCTCCTCTTTCCTTCTGTCAAAGCAAAA TATAGTGAAAGTCTCTGTTGTTACCTCAAACCTCCTTTCCTAATAAGTGTGCATGCCTGT AGATGGATTGGGATGCAGTGAACAGAGAAGTTAAACAAAAAGGTTAAAAAAAAAAAAAAA GGCCAGGCACAGTGGCTCACAACTGTAATCCCAGCACTCTGGGACGCTGAGGTGTGAGGA TCGCTTGAGCCCAGGAGTCTGAGATCTGCCTGGGCAACATAGGGAGACCCCCCACCTCTA CAAAAAAAAATTGAAAAAAATTATCTGGATATGAGGTGCACACCTGTGCTCCTAACTACT TGGGAGGCTGACGCCCGAGCGATCACCCGATCTGGTAAGGTCAAGGCTGCAATCACTTGT CACTGTGTCACTGCCCTTCTCCTTCGCGACATTGAAACTCTTTTTAACTATTTAGGCCAA TTCTCCTACCCCGTACCATGTTTTTTTATGAAAAAAGCCTCCTCCACCCCCCTTTTACCC TCCCCACCCTTCTTCTCTTATTTTTTTTTTTTCCTCTTCCCCCACCGTACTTCTATTAGT TTTATCCCCCCCCTTCTCTTCTTTTCTTTCTTTAATCCCCGCCCGTGTCTCATCTCCCAT CTCCCTTTTTCGTCTCTTCCCCTTCCCTTTTCCACTGTCCATCAATCCCCTCCTCTTTTT TAAACCCCTCCTTCCTTCTACTCAACCTCTCCCCCCACCCCCACCCCTCTTCTCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000947 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000947.2, NP_000938.2 |
| RefSeq Size | 2353 bp |
| RefSeq ORF | 1530 bp |
| Locus ID | 5558 |
| UniProt ID | P49643 |
| Domains | DNA_primase_lrg |
| Protein Pathways | DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism |
| Gene Summary | This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209566 | PRIM2 (Myc-DDK-tagged)-Human primase, DNA, polypeptide 2 (58kDa) (PRIM2) |
CNY 3,792.00 |
|
| RC209566L1 | Lenti ORF clone of Human primase, DNA, polypeptide 2 (58kDa) (PRIM2), Myc-DDK-tagged |
CNY 6,192.00 |
|
| RC209566L2 | Lenti ORF clone of Human primase, DNA, polypeptide 2 (58kDa) (PRIM2), mGFP tagged |
CNY 5,890.00 |
|
| RC209566L3 | Lenti ORF clone of Human primase, DNA, polypeptide 2 (58kDa) (PRIM2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209566L4 | Lenti ORF clone of Human primase, DNA, polypeptide 2 (58kDa) (PRIM2), mGFP tagged |
CNY 5,890.00 |
|
| RG209566 | PRIM2 (tGFP-tagged) - Human primase, DNA, polypeptide 2 (58kDa) (PRIM2) |
CNY 4,370.00 |
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