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Actin (ACTA1) (NM_001100) Human Untagged Clone
CAT＃: SC125333
ACTA1 (untagged)-Human actin, alpha 1, skeletal muscle (ACTA1)
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; SHPM |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001100, the custom clone sequence may differ by one or more nucleotides
ATGTGCGACGAAGACGAGACCACCGCCCTCGTGTGCGACAATGGCTCCGGCCTGGTGAAAGCCGGCTTCG CCGGGGATGACGCCCCTAGGGCCGTGTTCCCGTCCATCGTGGGCCGCCCCCGACACCAGGGCGTCATGGT CGGTATGGGTCAGAAAGATTCCTACGTGGGCGACGAGGCTCAGAGCAAGAGAGGTATCCTGACCCTGAAG TACCCTATCGAGCACGGCATCATCACCAACTGGGATGACATGGAGAAGATCTGGCACCACACCTTCTACA ACGAGCTTCGCGTGGCTCCCGAGGAGCACCCCACCCTGCTCACCGAGGCCCCCCTCAATCCCAAGGCCAA CCGCGAGAAGATGACCCAGATCATGTTTGAGACCTTCAACGTGCCCGCCATGTACGTGGCCATCCAGGCC GTGCTGTCCCTCTACGCCTCCGGCAGGACCACCGGCATCGTGCTGGACTCCGGCGACGGCGTCACCCACA ACGTGCCCATTTATGAGGGCTACGCGCTGCCGCACGCCATCATGCGCCTGGACCTGGCGGGCCGCGATCT CACCGACTACCTGATGAAGATCCTCACTGAGCGTGGCTACTCCTTCGTGACCACAGCTGAGCGCGAGATC GTGCGCGACATCAAGGAGAAGCTGTGCTACGTGGCCCTGGACTTCGAGAACGAGATGGCGACGGCCGCCT CCTCCTCCTCCCTGGAAAAGAGCTACGAGCTGCCAGACGGGCAGGTCATCACCATCGGCAACGAGCGCTT CCGCTGCCCGGAGACGCTCTTCCAGCCCTCCTTCATCGGTATGGAGTCGGCGGGCATTCACGAGACCACC TACAACAGCATCATGAAGTGTGACATCGACATCAGGAAGGACCTGTATGCCAACAACGTCATGTCGGGGG GCACCACGATGTACCCTGGGATCGCTGACCGCATGCAGAAAGAGATCACCGCGCTGGCACCCAGCACCAT GAAGATCAAGATCATCGCCCCGCCGGAGCGCAAATACTCGGTGTGGATCGGCGGCTCCATCCTGGCCTCG CTGTCCACCTTCCAGCAGATGTGGATCACCAAGCAGGAGTACGACGAGGCCGGCCCTTCCATCGTCCACC GCAAATGCTTCTAG >OriGene 5' read for NM_001100 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCCATTACGGCCGGGGACCGCAGCGGA CAGCGCCAAGTGAAGCCTCGCTTCCCTCCCGCGGCGACCAGGGCCCGAGCCGAGAGTAGC AGTTGTAGCTACCCGCCCAGAAACTAGACACAATGTGCGACGAAGACGAGACCACCGCCC TCGTGTGCGACAATGGCTCCGGCCTGGTGAAAGCCGGCTTCGCCGGGGATGACGCCCCTA GGGCCGTGTTCCCGTCCATCGTGGGCCGCCCCCGACACCAGGGCGTCATGGTCGGTATGG GTCAGAAAGATTCCTACGTGGGCGACGAGGCTCAGAGCAAGAGAGGTATCCTGACCCTGA AGTACCCTATCGAGCACGGCATCATCACCAACTGGGATGACATGGAGAAGATCTGGCACC ACACCTTCTACAACGAGCTTCGCGTGGCTCCCGAGGAGCACCCCACCCTGCTCACCGAGG CCCCCCTTCATCCCAAGGCCAACCGCGAGAAGATGACCAGATCATGTTTGGACCTTCACG TGCCGCATGTACTGGCATCAAGCGTGGTGGCCTTTAGGCTTCGAGACAACCGTTTGGGTT GATCGGGCAGGCGTACCCCACGTGCCATTATGAGCTAGCGTTGCGAGCATTTGCCTGACT GCGGCGGTTTACGATCTGTAATACTATGACGGTTC |
| Restriction Sites | Please inquire |
| ACCN | NM_001100 |
| Insert Size | 1600 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001100.3, NP_001091.1 |
| RefSeq Size | 1509 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 58 |
| UniProt ID | P68133 |
| Domains | ACTIN |
| Protein Families | Stem cell - Pluripotency |
| Gene Summary | The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause a variety of myopathies, including nemaline myopathy, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects with manifestations such as hypotonia. [provided by RefSeq, Sep 2019] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Therapeutic impact of systemic AAV-mediated RNA interference in a mouse model of myotonic dystrophy
,Bisset, DR;Stepniak-Konieczna, EA;Zavaljevski, M;Wei, J;Carter, GT;Weiss, MD;Chamberlain, JR;,
Hum. Mol. Genet.
,PubMed ID 26082468
[ACTA1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203112 | ACTA1 (Myc-DDK-tagged)-Human actin, alpha 1, skeletal muscle (ACTA1) |
CNY 5,488.00 |
|
| RC203112L3 | Lenti-ORF clone of ACTA1 (Myc-DDK-tagged)-Human actin, alpha 1, skeletal muscle (ACTA1) |
CNY 5,890.00 |
|
| RC203112L4 | Lenti-ORF clone of ACTA1 (mGFP-tagged)-Human actin, alpha 1, skeletal muscle (ACTA1) |
CNY 5,890.00 |
|
| RG203112 | ACTA1 (tGFP-tagged) - Human actin, alpha 1, skeletal muscle (ACTA1) |
CNY 7,088.00 |
