United States
Germany
Japan
United Kingdom
China
ACAT1 (NM_000019) Human Untagged Clone
CAT＃: SC125282
ACAT1 (untagged)-Human acetyl-CoA acetyltransferase 1 (ACAT1), nuclear gene encoding mitochondrial protein
CNY 5,488.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACAT; MAT; T2; THIL |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC125282 sequence for NM_000019 edited (data generated by NextGen Sequencing)
ATGGCTGTGCTGCCGGCACTTCTGCGCAGCGGCGCCCGCAGCCGCAGCCCCCTGCTCCGG AGGCTGGTGCAGGAAATAAGATATGTGGAACGGAGTTATGTATCAAAACCCACTTTGAAG GAAGTGGTCATAGTAAGTGCTACAAGAACACCCATTGGATCTTTTTTAGGCAGCCTTTCC TTGCTGCCAGCCACTAAGCTTGGTTCCATTGCAATTCAGGGAGCCATTGAAAAGGCAGGG ATTCCAAAAGAAGAAGTGAAAGAAGCATACATGGGTAATGTTCTACAAGGAGGTGAAGGA CAAGCTCCTACAAGGCAGGCAGTATTGGGTGCAGGCTTACCTATTTCTACTCCATGTACC ACCATAAACAAAGTTTGTGCTTCAGGAATGAAAGCCATCATGATGGCCTCTCAAAGTCTT ATGTGTGGACATCAGGATGTGATGGTGGCAGGTGGGATGGAGAGCATGTCCAATGTTCCA TATGTAATGAACAGAGGATCAACACCATATGGTGGGGTAAAGCTTGAAGATTTGATTGTA AAAGACGGGCTAACTGATGTCTACAATAAAATTCATATGGGCAGCTGTGCTGAGAATACA GCAAAGAAGCTGAATATTGCACGAAATGAACAGGACGCTTATGCTATTAATTCTTATACC AGAAGTAAAGCAGCATGGGAAGCTGGGAAATTTGGAAATGAAGTTATTCCTGTCACAGTT ACAGTAAAAGGTCAACCAGATGTAGTGGTGAAAGAAGATGAAGAATATAAACGTGTTGAT TTTAGCAAAGTTCCAAAGCTGAAGACAGTTTTCCAGAAAGAAAATGGCACAGTAACAGCT GCCAATGCCAGTACACTGAATGATGGAGCAGCTGCTCTGGTTCTCATGACGGCAGATGCA GCGAAGAGGCTCAATGTTACACCACTGGCAAGAATAGTAGCATTTGCTGACGCTGCTGTA GAACCTATTGATTTTCCAATTGCTCCTGTATATGCTGCATCTATGGTTCTTAAAGATGTG GGATTGAAAAAAGAAGATATTGCAATGTGGGAAGTAAATGAAGCCTTTAGTCTGGTTGTA CTAGCAAACATTAAAATGTTGGAGATTGATCCCCAAAAAGTGAATATCAATGGAGGAGCT GTTTCTCTGGGACATCCAATTGGGATGTCTGGAGCCAGGATTGTTGGTCATTTGACTCAT GCCTTGAAGCAAGGAGAATACGGTCTTGCCAGTATTTGCAATGGAGGAGGAGGTGCTTCT GCCATGCTAATTCAGAAGCTGTAG Clone variation with respect to NM_000019.3 13 g=>c >OriGene 5' read for NM_000019 unedited
ATTTTGTATCCGACTTCTATAGGCGGCCGCGCAATTCGCACGAGTGGAGCCGATACTCAT CCCACTGCGACCATGGCTGTGCTGCCGGCACTTCTGCGCATCGGCGCCCGCAGCCGCAGC CCCCTGCTCCGGAGGCTGGTGCAGGAAATAAGATATGTGGAACGGAGTTATGTATCAAAA CCCACTTTGAAGGAAGTGGTCATAGTAAGTGCTACAAGAACACCCATTGGATCTTTTTTA AGCAGCCTTTCCTTGCTGCCAGCCACTAAGCTTGGTTCCATTGCAATTCAAGGAGCCATT GAAAAGGCAGGGATTCCAAAAGAAGAAGTGAAAGAAGCATACATGGGTAATGTTCTACAA AGAGGTGAAAGACAAGCTCCTACAAAGCAAGCAGTATTGGGTGCAAGCTTACCTATTTCT ACTCCATGTACCACCATAAACAAAGTTTGTGCTTCAAGAATGAAAGCCATCATGATGGCC TCTCAAAGTCTTATGTGTGGACATCAAGATGTGATGGTGGCAGGTGGGATGGATAGCATG TCCAATGTTCCATATGTAATGAACAGATGATCAACACCATATGGTGGGGTAAAGCTTGAA GATTTGATTGCTAAAGACGGGCTAACTGATGTCTACAATAAAATTCATATGGGCAGCTGT GCTGAGAATACAGCANAGAATCTGAATATTGCACGAAATGAACAGGACGGCTATGCTATT AATTCCTATACCANAAGTAAAGCAGCATGGGAAGCTGGGAAATTTGCAAATGAAGNTATT CCTGTCACAGTTACAGTAAAAAGTCAACCANATGTATCGGCAGAAAGAGAATGAGAATAT AAACGCGTGATCTTAACAAGTTCCAAAGCTGAAGACAGTCTTCCCGAAAGAAAATGCCCA GTNACAGCTGTCAATTGCCTNACACTGAATGATGGAGCAACTGCTCCTGTTCTCATGACG CANATCCCGGAATAGCTCATGTTCCCACTGCCAAAATAAACCTGGCGGCCCGTCGTAAAT CTATGATTTCAATGC >OriGene 3' genomic read for NM_000019 unedited
AGNCACATAACAAGNCCTCAAAATTGGTGATGATTACAGAGTTTTTGAAAAACTCTACTC TTCCTCTGAATCTGAAGAGCAATCTAATCTATTTCTCCCATATATTGTCATTTTGCTCCT TCAAAAGATCTACAATACATTATTTGCCAACAGATAATGTGTACACAATGACCTTTACTG CCCCCTCCTCTTCCTGAGGGGGAGTTTTCCCTCTTCAGAGCTGCAATTGAGGGTAGTGTG CATAGCCCCTGCCTGTAGCGTTTTAATTAATTTTTTCCCAGATAAAATGCAATGCGAGAT ACTTCTGTTACAGTCTGTCCTGCTTAAGACAGGCATTGTCGTGTATTGGAAGTGGTGATC TGTTCCCTTTTTTATATATCCATTCAGCATATCAGTTTCTACCCTCCCTTCCACTCTCTA TGCTGCTATAGTAATTCAGAGCCCAAACTCCAGGGTTTTCTGCTTGACCCTCCCCCAACC CTGAATGTTGAGGTTATAAGAAAATGCCCCCCCTCTAGACCCTGAGGACATGGGGCTTCT GTCCTTGTTTCTAATACTGCTGGCCCACACTGGGGTTTTGGAGTGATTATATTAAGCCAC TGCTAAGTTTGTCCCAAAATACCCACTTCTAACTGGAAAAAAAAAATAATTTTAATTTAC CCGGAATTTACCTAAATGTAAATTAACCTTANAAAAGCCATTCCTTTGACCCTTTAAAAA TCCTAAAAAAATTAAAGGGGTTCTCAACAATGGAAACCTTCTGCCCCCTATTTTAAGCCC CTAATTTTCCCCCTTGGGATTTTTCCCCTATTCCGAAAAGGTAGACCTCCAAAGGCAAAA TGTTTCAAACTTTTCAGGGTTCCCCGAAAAAACCCCAAAAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000019 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000019.2, NP_000010.1 |
| RefSeq Size | 1518 bp |
| RefSeq ORF | 1284 bp |
| Locus ID | 38 |
| UniProt ID | P24752 |
| Domains | thiolase |
| Protein Families | Druggable Genome |
| Protein Pathways | Butanoate metabolism, Fatty acid metabolism, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Tryptophan metabolism, Valine, leucine and isoleucine degradation |
| Gene Summary | This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217753 | ACAT1 (Myc-DDK-tagged)-Human acetyl-CoA acetyltransferase 1 (ACAT1), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
|
| RC217753L1 | Lenti-ORF clone of ACAT1 (Myc-DDK-tagged)-Human acetyl-CoA acetyltransferase 1 (ACAT1), nuclear gene encoding mitochondrial protein |
CNY 7,888.00 |
|
| RC217753L2 | Lenti-ORF clone of ACAT1 (mGFP-tagged)-Human acetyl-CoA acetyltransferase 1 (ACAT1), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
| RC217753L3 | Lenti-ORF clone of ACAT1 (Myc-DDK-tagged)-Human acetyl-CoA acetyltransferase 1 (ACAT1), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
| RC217753L4 | Lenti-ORF clone of ACAT1 (mGFP-tagged)-Human acetyl-CoA acetyltransferase 1 (ACAT1), nuclear gene encoding mitochondrial protein |
CNY 7,888.00 |
|
| RG217753 | ACAT1 (tGFP-tagged) - Human acetyl-CoA acetyltransferase 1 (ACAT1), nuclear gene encoding mitochondrial protein |
CNY 7,088.00 |
