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NRG1 (NM_013956) Human Untagged Clone
CAT＃: SC124810
NRG1 (untagged)-Human neuregulin 1 (NRG1), transcript variant HRG-beta1
CN¥ 7,216.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; NRG1-IT2; SMDF |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC124810 sequence for NM_013956 edited (data generated by NextGen Sequencing)
ATGTCCGAGCGCAAAGAAGGCAGAGGCAAAGGGAAGGGCAAGAAGAAGGAGCGAGGCTCC GGCAAGAAGCCGGAGTCCGCGGCGGGCAGCCAGAGCCCAGCCTTGCCTCCCCAATTGAAA GAGATGAAAAGCCAGGAATCGGCTGCAGGTTCCAAACTAGTCCTTCGGTGTGAAACCAGT TCTGAATACTCCTCTCTCAGATTCAAGTGGTTCAAGAATGGGAATGAATTGAATCGAAAA AACAAACCACAAAATATCAAGATACAAAAAAAGCCAGGGAAGTCAGAACTTCGCATTAAC AAAGCATCACTGGCTGATTCTGGAGAGTATATGTGCAAAGTGATCAGCAAATTAGGAAAT GACAGTGCCTCTGCCAATATCACCATCGTGGAATCAAACGAGATCATCACTGGTATGCCA GCCTCAACTGAAGGAGCATATGTGTCTTCAGAGTCTCCCATTAGAATATCAGTATCCACA GAAGGAGCAAATACTTCTTCATCTACATCTACATCCACCACTGGGACAAGCCATCTTGTA AAATGTGCGGAGAAGGAGAAAACTTTCTGTGTGAATGGAGGGGAGTGCTTCATGGTGAAA GACCTTTCAAACCCCTCGAGATACTTGTGCAAGTGCCCAAATGAGTTTACTGGTGATCGC TGCCAAAACTACGTAATGGCCAGCTTCTACAAGCATCTTGGGATTGAATTTATGGAGGCG GAGGAGCTGTACCAGAAGAGAGTGCTGACCATAACCGGCATCTGCATCGCCCTCCTTGTG GTCGGCATCATGTGTGTGGTGGCCTACTGCAAAACCAAGAAACAGCGGAAAAAGCTGCAT GACCGTCTTCGGCAGAGCCTTCGGTCTGAACGAAACAATATGATGAACATTGCCAATGGG CCTCACCATCCTAACCCACCCCCCGAGAATGTCCAGCTGGTGAATCAATACGTATCTAAA AACGTCATCTCCAGTGAGCATATTGTTGAGAGAGAAGCAGAGACATCCTTTTCCACCAGT CACTATACTTCCACAGCCCATCACTCCACTACTGTCACCCAGACTCCTAGCCACAGCTGG AGCAACGGACACACTGAAAGCATCCTTTCCGAAAGCCACTCTGTAATCGTGATGTCATCC GTAGAAAACAGTAGGCACAGCAGCCCAACTGGGGGCCCAAGAGGACGTCTTAATGGCACA GGAGGCCCTCGTGAATGTAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGATTCCTAC CGAGACTCTCCTCATAGTGAAAGGTATGTGTCAGCCATGACCACCCCGGCTCGTATGTCA CCTGTAGATTTCCACACGCCAAGCTCCCCCAAATCGCCCCCTTCGGAAATGTCTCCACCC GTGTCCAGCATGACGGTGTCCATGCCTTCCATGGCGGTCAGCCCCTTCATGGAAGAAGAG AGACCTCTACTTCTCGTGACACCACCAAGGCTGCGGGAGAAGAAGTTTGACCATCACCCT CAGCAGTTCAGCTCCTTCCACCACAACCCCGCGCATGACAGTAACAGCCTCCCTGCTAGC CCCTTGAGGATAGTGGAGGATGAGGAGTATGAAACGACCCAAGAGTACGAGCCAGCCCAA GAGCCTGTTAAGAAACTCGCCAATAGCCGGCGGGCCAAAAGAACCAAGCCCAATGGCCAC ATTGCTAACAGATTGGAAGTGGACAGCAACACAAGCTCCCAGAGCAGTAACTCAGAGAGT GAAACAGAAGATGAAAGAGTAGGTGAAGATACGCCTTTCCTGGGCATACAGAACCCCCTG GCAGCCAGTCTTGAGGCAACACCTGCCTTCCGCCTGGCTGACAGCAGGACTAACCCAGCA GTCCGCTTCTCGACACAGGAAGAAATCCAGGCCAGGCTGTCTAGTGTAATTGCTAACCAA GACCCTATTGCTGTATAA Clone variation with respect to NM_013956.3 113 g=>a;1862 g=>t >OriGene 5' read for NM_013956 unedited
ATACGACTCCTATAGGGCGGCCGCGAATTCGCCGAGGGGCTCGCGCGGAGGCCAGGAGCT GAGCGGCGGCGGCTGCCGGACGATGGGAGCGTGAGCAGGACGGTGATAACCTCTCCCCGA TCGGGTTGCGAGGGCGCCGGGCAGAGGCCAGGACGCGAGCCGCCAGCGGCGGGACCCATC GACGACTTCCCGGGGCGACAGGAGCAGCCCCGAGAGCCAGGGCGAGCGCCCGTTCCAGGT GGCCGGACCGCCCGCCGCGTCCGCGCCGCGCTCCCTGCAGGCAACGGGAGACGCCCCCGC GCAGCGCGAGCGCCTCAGCGCGGCCGCTCGCTCTCCCCATCGAGGGACAAACTTTTCCCA AACCCGATCCGAGCCCTTGGACCAAACTCGCCTGCGCCGAGAGCCGTCCGCGTAGAGCGC TCCGTCTCCGGCGAGATGTCCGAGCGCAAAGAAGGCAGAGGCAAAGGGAAGGGCAAGAAG AAGGAGCGAGGCTCCGGCAAGAAGCCGGAGTCCGCGGCGGGCAGCCAGAGCCCAGCCTTG CCTCCCCAATTGAAAGAGATGAAAAGCCAGGAATCGGCTGCAGGTTCCAAACTAGTCCTT CGGTGTGAAACCAGTTCTGAATACTCCTCTCTCAGATTCAAGTGGTTCAAGAATGGGAAT GAATTGAATCGAANAAACAAACCACAAAATATCAAGATACAAAAAAAGCCAGNGGAAGTC AGAACTTCGCATTAACAAAGCATCACTGGCTGATTCTGGAGAGTATATGTGCANAGTGAT CAGCAATTAGGAAATGACAGTGCCTCTGCCATATCACCATCGTGGATCAAACGAGATCTC ACTGGTATGCCAGNCTCACTGAAGAGCCTAATGTGCT >Forward primer walk for NM_013956 unedited
NTTCTCCGCGAAAAAATCAGGCACANCAGCCCACTGGGGGCCCAAAAGGACGTCTTAATG GCACAGGAGGCCCTNCGTGATGTAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGATT CCTACCGAGACTCTCCTCATAGTGAAAGGTATGTGTCAGCCATGACCACCCCGGCTCGTA TGTCACCTGTAGATTTCCACACGCCAAGCTCCCCCAAATCGCCCCCTTCGGAAATGTCTC CACCCGTGTCCAGCATGACGGTGTCCATGCCTTCCATGGCGGTCAGCCCCTTCATGGAAG AAGAGAGACCTCTACTTCTCGTGACACCACCAAGGCTGCGGGAGAAGAAGTTTGACCATC ACCCTCAGCAGTTCAGCTCCTTCCACCACAACCCCGCGCATGACAGTAACAGCCTCCCTG CTAGCCCCTTGAGGATAGTGGAGGATGAGGAGTATGAAACGACCCAAGAGTACGAGCCAG CCCAAGAGCCTGTTAAGAAACTCGCCAATAGCCGGCGGGCCAAAAGACCAAGCCCAATGG CCACATTGCTAACAGATTGGAAGTGGACAGCAACACAAGCTCCCAGAGCAGTAACTCAGA GAGTGAAACAGAAGATGAAAGAGTAGGTGAAGATACGCCTTTCCTGGGCATACAGAACCC CCTGGCAGCCAGTCTTGAGGCAACACCTGCCTTCCGCCTGGCTGACAGCAGGACTAACCC CGCAGTTCCGCTTCTCGACACAGGAAAAAATCCAGGCCACAGCTGGCTAGTGGTAATTGG TAACCCAAAAACCCTTTTGCTGGTTTAAAACCTAAATAAACCCCTTGGATTTCCCTGGGA AAACTTTATTTTTATTTTAAAAAGGTTTTTCCCCTCTAAAATAAAAACAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_013956 |
| Insert Size | 4100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013956.2, NP_039250.2 |
| RefSeq Size | 2583 bp |
| RefSeq ORF | 1938 bp |
| Locus ID | 3084 |
| UniProt ID | Q02297 |
| Protein Families | Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane |
| Protein Pathways | ErbB signaling pathway |
| Gene Summary | The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Apr 2016] Transcript Variant: This variant (HRG-beta1), which uses the type I promoter, encodes isoform HRG-beta1 (also known as NDF-beta1a or HRG-beta1a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Anti-HER3 monoclonal antibody patritumab sensitizes refractory non-small cell lung cancer to the epidermal growth factor receptor inhibitor erlotinib
,Yonesaka, K;Hirotani, K;Kawakami, H;Takeda, M;Kaneda, H;Sakai, K;Okamoto, I;Nishio, K;Jänne, PA;Nakagawa, K;,
Oncogene
,PubMed ID 25961915
[NRG1]
|
|
Computational Modeling of ERBB2-Amplified Breast Cancer Identifies Combined ErbB2/3 Blockade as Superior to the Combination of MEK and AKT Inhibitors
,Daniel C. Kirouac, Jin Y. Du, Johanna Lahdenranta, Ryan Overland, Defne Yarar, Violette Paragas, Emily Pace, Charlotte F. McDonagh, Ulrik B. Nielsen, and Matthew D. Onsum,
Sci. Signal., Aug 2013; 6: ra68.
,PubMed ID 23943608
[NRG1]
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