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GLP1R (NM_002062) Human Untagged Clone
CAT＃: SC124060
GLP1R (untagged)-Human glucagon-like peptide 1 receptor (GLP1R)
CNY 5,488.00
| Cited in 5 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GLP-1; GLP-1-R; GLP-1R |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC124060 sequence for NM_002062 edited (data generated by NextGen Sequencing)
ATGGCCGGCGCCCCCGGCCCGCTGCGCCTTGCGCTGCTGCTGCTCGGGATGGTGGGCAGG GCCGGCCCCCGCCCCCAGGGTGCCACTGTGTCCCTCTGGGAGACGGTGCAGAAATGGCGA GAATACCGACGCCAGTGCCAGCGCTCCCTGACTGAGGATCCACCTCCTGCCACAGACTTG TTCTGCAACCGGACCTTCGATGAATACGCCTGCTGGCCAGATGGGGAGCCAGGCTCGTTC GTGAATGTCAGCTGCCCCTGGTACCTGCCCTGGGCCAGCAGTGTGCCGCAGGGCCACGTG TACCGGTTCTGCACAGCTGAAGGCCTCTGGCTGCAGAAGGACAACTCCAGCCTGCCCTGG AGGGACTTGTCGGAGTGCGAGGAGTCCAAGCGAGGGGAGAGAAGCTCCCCGGAGGAGCAG CTCCTGTTCCTCTACATCATCTACACGGTGGGCTACGCACTCTCCTTCTCTGCTCTGGTT ATCGCCTCTGCGATCCTCCTCGGCTTCAGACACCTGCACTGCACCAGGAACTACATCCAC CTGAACCTGTTTGCATCCTTCATCCTGCGAGCATTGTCCGTCTTCATCAAGGACGCAGCC CTGAAGTGGATGTATAGCACAGCCGCCCAGCAGCACCAGTGGGATGGGCTCCTCTCCTAC CAGGACTCTCTGAGCTGCCGCCTGGTGTTTCTGCTCATGCAGTACTGTGTGGCGGCCAAT TACTACTGGCTCTTGGTGGAGGGCGTGTACCTGTACACACTGCTGGCCTTCTCGGTCTTC TCTGAGCAATGGATCTTCAGGCTCTACGTGAGCATAGGCTGGGGTGTTCCCCTGCTGTTT GTTGTCCCCTGGGGCATTGTCAAGTACCTCTATGAGGACGAGGGCTGCTGGACCAGGAAC TCCAACATGAACTACTGGCTCATTATCCGGCTGCCCATTCTCTTTGGCATTGGGGTGAAC TTCCTCATCTTTGTTCGGGTCATCTGCATCGTGGTATCCAAACTGAAGGCCAATCTCATG TGCAAGACAGACATCAAATGCAGACTTGCCAAGTCCACGCTGACACTCATCCCCCTGCTG GGGACTCATGAGGTCATCTTTGCCTTTGTGATGGACGAGCACGCCCGGGGGACCCTGCGC TTCATCAAGCTGTTTACAGAGCTCTCCTTCACCTCCTTCCAGGGGCTGATGGTGGCCATA TTATACTGCTTTGTCAACAATGAGGTCCAGCTGGAATTTCGGAAGAGCTGGGAGCGCTGG CGGCTTGAGCACTTGCACATCCAGAGGGACAGCAGCATGAAGCCCCTCAAGTGTCCCACC AGCAGCCTGAGCAGTGGAGCCACGGCGGGCAGCAGCATGTACACAGCCACTTGCCAGGCC TCCTGCAGCTGA Clone variation with respect to NM_002062.3 399 a=>g;780 a=>c;947 c=>g >OriGene 5' read for NM_002062 unedited
GAGTTANGATTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGCCCTTGATGGCCC AGTCCTGAACTCCCCGCCATGGCCGGCGCCCCCGGCCCGCTGCGCCTTGCGCTGCTGCTG CTCGGGATGGTGGGCAGGGCCGGCCCCCGCCCCCAGGGTGCCACTGTGTCCCTCTGGGAG ACGGTGCAGAAATGGCGAGAATACCGACGCCAGTGCCAGCGCTCCCTGACTGAGGATCCA CCTCCTGCCACAGACTTGTTCTGCAACCGGACCTTCGATGAATACGCCTGCTGGCCAGAT GGGGAGCCAGGCTCGTTCGTGAATGTCAGCTGCCCCTGGTACCTGCCCTGGGCCAGCAGT GTGCCGCAGGGCCACGTGTACCGGTTCTGCACAGCTGAAGGCCTCTGGCTGCAGAAGGAC AACTCCAGCCTGCCCTGGAGGGACTTGTCGGAGTGCGAGGAGTCCAAGCGAGGGGAGAGA AGCTCCCCGGAGGAGCAGCTCCTGTTCCTCTACATCATCTACACGGTGGGCTACGCACTC TCCTTCTCTGCTCTGGTTATCGCCTCTGCGATCCTCCTCGGCTTCAGACACCTGCACTGC ACCANGAACTACATCCACCTGAACCTGTTTGCATCCTTCATCCTGCGAGCATTGTCCGTC TTCATCAAGGACGCAGCCCTGAAGTGGATGTATAGCACAGCCGCCCAGCAGCACCAGTGG GATGGGCTCCTCTNNCTACCAGACTCTCTGAGCTGCCGCCTGGTGTTTCTGCTCATGCAG TACTGTGTGGCGGCCAAATACTACTGGCTCTTGGTGGAGGGCGTGTACCTGTACCACTGC TGGGCTTCTCGGGTCTCTCTGAGCAATGGATCTTCAGGCTCTACGTGAGCATANGCTGGG GGTGTTTCCCTGC >Forward primer walk for NM_002062 unedited
AAGTCAGCTGACATCACCCCTGCTGGGGACTCATGAGGTCATCTTTGCCTTTGTGATGGA CGAGCACGCCCCGGGGGACCCTGCGCTTCATCAAGCTGTTTACAGAGCTCTCCTTCACCT CCTTCCAGGGGCTGATGGTGGCCATATTATACTGCTTTGTCAACAATGAGGTCCAGCTGG AATTTCGGAAGAGCTGGGAGCGCTGGCGGCTTGAGCACTTGCACATCCAGAGGGACAGCA GCATGAAGCCCCTCAAGTGTCCCACCAGCAGCCTGAGCAGTGGAGCCACGGCGGGCAGCA GCATGTACACAGCCACTTGCCAGGCCTCCTGCAGCTGAGACTCCAGCGCCTGCCCTCCCT GGGGTCCTTGCTGCAGGCCTGGTGGCCAATCCAGGTGGGAGAGACACTCAAGGGCGAATT CAGATCTGGTACCGATATCAAGCTTGTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTT TCCTGAACAGATCCCCGGTGGCATCCCTGTGACCNCTCCCCAGTGCCTCTCCTGGCCCTT GGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAGTTGCATCATTTTG TCTGACTANGTGTCCTTCTTAAATGGAGGGG |
| Restriction Sites | Please inquire |
| ACCN | NM_002062 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002062.2, NP_002053.2 |
| RefSeq Size | 3071 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 2740 |
| UniProt ID | P43220 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| Gene Summary | This gene encodes a 7-transmembrane protein that functions as a receptor for glucagon-like peptide 1 (GLP-1) hormone, which stimulates glucose-induced insulin secretion. This receptor, which functions at the cell surface, becomes internalized in response to GLP-1 and GLP-1 analogs, and it plays an important role in the signaling cascades leading to insulin secretion. It also displays neuroprotective effects in animal models. Polymorphisms in this gene are associated with diabetes. The protein is an important drug target for the treatment of type 2 diabetes and stroke. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the supported protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Biased GLP-2 agonist with strong G protein-coupling but impaired arrestin recruitment and receptor desensitization enhances intestinal growth in mice.
,null,
British journal of pharmacology
,PubMed ID 36683195
[GLP1R]
|
|
N‐terminal alterations turn the gut hormone GLP‐2 into an antagonist with gradual loss of GLP‐2 receptor selectivity towards more GLP‐1 receptor interaction
,null,
British Journal of Pharmacology
,PubMed ID 35523760
[GLP1R]
|
|
A proopiomelanocortin‐derived peptide sequence enhances plasma stability of peptide drugs
,null,
FEBS Letters
,PubMed ID 32506501
[GLP1R]
|
|
Modulation of Glucagon-like Peptide (GLP)-1 Potency by Endocannabinoid-like Lipids Represents A Novel Mode of Regulating GLP-1 Receptor Signaling
,Cheng, YH;Ho, MS;Huang, WT;Chou, YT;King, K;,
J. Biol. Chem.
,PubMed ID 25903129
[GLP1R]
|
|
Synthesis and biological evaluation of glucagon-like peptide-1 receptor agonists
,Zhang, YJ;Shen, LL;Cheon, HG;Xu, YN;Jeong, JH;,
Arch. Pharm. Res.
,PubMed ID 24178744
[GLP1R]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211333 | GLP1R (Myc-DDK-tagged)-Human glucagon-like peptide 1 receptor (GLP1R) |
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| RC211333L1 | Lenti ORF clone of Human glucagon-like peptide 1 receptor (GLP1R), Myc-DDK-tagged |
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| RC211333L2 | Lenti ORF clone of Human glucagon-like peptide 1 receptor (GLP1R), mGFP tagged |
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| RC211333L3 | Lenti ORF clone of Human glucagon-like peptide 1 receptor (GLP1R), Myc-DDK-tagged |
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| RC211333L4 | Lenti ORF clone of Human glucagon-like peptide 1 receptor (GLP1R), mGFP tagged |
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| RG211333 | GLP1R (tGFP-tagged) - Human glucagon-like peptide 1 receptor (GLP1R) |
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