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NF2 (NM_000268) Human Untagged Clone
CAT＃: SC124024
NF2 (untagged)-Human neurofibromin 2 (merlin) (NF2), transcript variant 1
CNY 6,656.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACN; BANF; merlin-1; SCH |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC124024 sequence for NM_000268 edited (data generated by NextGen Sequencing)
ATGGCCGGGGCCATCGCTTCCCGCATGAGCTTCAGCTCTCTCAAGAGGAAGCAACCCAAG ACGTTCACCGTGAGGATCGTCACCATGGACGCCGAGATGGAGTTCAATTGCGAGATGAAG TGGAAAGGGAAGGACCTCTTTGATTTGGTGTGCCGGACTCTGGGGCTCCGAGAAACCTGG TTCTTTGGACTGCAGTACACAATCAAGGACACAGTGGCCTGGCTCAAAATGGACAAGAAG GTACTGGATCATGATGTTTCAAAGGAAGAACCAGTCACCTTTCACTTCTTGGCCAAATTT TATCCTGAGAATGCTGAAGAGGAGCTGGTTCAGGAGATCACACAACATTTATTCTTCTTA CAGGTAAAGAAGCAGATTTTAGATGAAAAGATCTACTGCCCTCCTGAGGCTTCTGTGCTC CTGGCTTCTTACGCCGTCCAGGCCAAGTATGGTGACTACGACCCCAGTGTTCACAAGCGG GGATTTTTGGCCCAAGAGGAATTGCTTCCAAAAAGGGTAATAAATCTGTATCAGATGACT CCGGAAATGTGGGAGGAGAGAATTACTGCTTGGTACGCAGAGCACCGAGGCCGAGCCAGG GATGAAGCTGAAATGGAATATCTGAAGATAGCTCAGGACCTGGAGATGTACGGTGTGAAC TACTTTGCAATCCGGAATAAAAAGGGCACAGAGCTGCTGCTTGGAGTGGATGCCCTGGGG CTTCACATTTATGACCCTGAGAACAGACTGACCCCCAAGATCTCCTTCCCGTGGAATGAA ATCCGAAACATCTCGTACAGTGACAAGGAGTTTACTATTAAACCACTGGATAAGAAAATT GATGTCTTCAAGTTTAACTCCTCAAAGCTTCGTGTTAATAAGCTGATTCTCCAGCTATGT ATCGGGAACCATGATCTATTTATGAGGAGAAGGAAAGCCGATTCTTTGGAAGTTCAGCAG ATGAAAGCCCAGGCCAGGGAGGAGAAGGCTAGAAAGCAGATGGAGCGGCAGCGCCTCGCT CGAGAGAAGCAGATGAGGGAGGAGGCTGAACGCACGAGGGATGAGTTGGAGAGGAGGCTG CTGCAGATGAAAGAAGAAGCAACAATGGCCAACGAAGCACTGATGCGGTCTGAGGAGACA GCTGACCTGTTGGCTGAAAAGGCCCAGATCACCGAGGAGGAGGCAAAACTTCTGGCCCAG AAGGCCGCAGAGGCTGAGCAGGAAATGCAGCGCATCAAGGCCACAGCGATTCGCACGGAG GAGGAGAAGCGCCTGATGGAGCAGAAGGTGCTGGAAGCCGAGGTGCTGGCACTGAAGATG GCTGAGGAGTCAGAGAGGAGGGCCAAAGAGGCAGATCAGCTGAAGCAGGACCTGCAGGAA GCACGCGAGGCGGAGCGAAGAGCCAAGCAGAAGCTCCTGGAGATTGCCACCAAGCCCACG TACCCGCCCATGAACCCAATTCCAGCACCGTTGCCTCCTGACATACCAAGCTTCAACCTC ATTGGTGACAGCCTGTCTTTCGACTTCAAAGATACTGACATGAAGCGGCTTTCCATGGAG ATAGAGAAAGAAAAAGTGGAATACATGGAAAAGAGCAAGCATCTGCAGGAGCAGCTCAAT GAACTCAAGACAGAAATCGAGGCCTTGAAACTGAAAGAGAGGGAGACAGCTCTGGATATT CTGCACAATGAGAACTCCGACAGGGGTGGCAGCAGCAAGCACAATACCATTAAAAAGCTC ACCTTGCAGAGCGCCAAGTCCCGAGTGGCCTTCTTTGAAGAGCTCTAG Clone variation with respect to NM_000268.3 >OriGene 5' read for NM_000268 unedited
AATACGACTCACTATAGGGCGGCCGCGAGTTCGGCACGAGGTTCCCGGGCCGGCCAGCCG GCCACCATGGTGGCCCTGAGGCCTGTGCAGCAACTCCAGGGGGGCTAAAGGGCTCANAGT GCAGGCCGTGGGGCGCGAGGGTCCCGGGCCTGAGCCCCGCGCCATGGCCGGGGCCATCGC TTCCCGCATGAGCTTCAGCTCTCTCAAGAGGAAGCAACCCAAGACGTTCACCGTGAGGAT CGTCACCATGGACGCCGAGATGGAGTTCAATTGCGAGATGAAGTGGAAAGGGAAGGACCT CTTTGATTTGGTGTGCCGGACTCTGGGGCTCCGAGAAACCTGGTTCTTTGGACTGCAGTA CACAATCAAGGACACAGTGGCCTGGCTCAAAATGGACAAGAAGGTACTGGATCATGATGT TTCAAAGGAAGAACCAGTCACCTTTCACTTCTTGGCCAAATTTTATCCTGAGAATGCTGA AGAGGAGCTGGTTCAGGAGATCACACAACATTTATTCTTCTTACAGGTAAAGAAGCAGAT TTTAGATGAAAAGATCTACTGCCCTCCTGAGGCTTCTGTGCTCCTGGCTTCTTACGCCGT CCAGGCCAAGTATGGTGACTACGACCCCAGTGTTCACAAGCGGGGATTTTTGGCCCAAGA GGAATTGCTTCCAAAAAGGGTAATAAATCTGTATCAGATGACTCCGGAAATGTGGGAGGA GAGAATTACTGCT >OriGene 3' read for NM_000268 unedited
ACTTCCAGGGCCAGGAGAGGCACTGGGGAGNGGTCACAGGGATGCCACCCGGGATCTGTT CAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTGGGTTTTTTTT TTTTTTAGACAAAGGAAGACAAATACTTCTTAAAACAAAGACTCAGGTCAGAGAACTAGA ACGCCATGAGAGAAATCTCTACAGGGTCGTAGTTCAAGGCAATTGCACATAAGAGGGGCT GGGCTCTTCACTCAGCTGGGGAAAGTTCTGGAGCTCCCACGGAAACCCCCAGCCAGCTCC CTATGGATGGCTCTCTTGATATCTGGTCCATCCCGCGGTCCCGGTAGCAGGAGAAGTGGC AGGTCCTGGGGTGGCTGGGTCACCTGCTAGAGCTCTTCAAAGAAGGCCACTCGGGACTTG GCGCTCTGCAAGGTGAGCTTTTTAATGGTATTGTGCTTGCTGCTGCCACCCCTGTCGGAG TTCTCATTGTGCAGAATATCCAGAGCTGTCTCCCTCTCTTTCAGTTTCAAGGCCTCGATT TCTGTCTTGAGTTCATTGAGCTGCTCCTGCAGATGCTTGCTCTTTTCCATGTATTCCACT TTTTCTTTCTCTATCTCCATGGAAAGCCGCTTCATGTCAGTATCTTTGAAGTCGAAAGAC AGGCTGTCACCAATGAGGTTGAAGCTTGGTATGTCAAGAGGCAACGGTGCTGGAATTGGG TTCATGGGCGGGT |
| Restriction Sites | Please inquire |
| ACCN | NM_000268 |
| Insert Size | 2300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000268.2, NP_000259.1 |
| RefSeq Size | 6021 bp |
| RefSeq ORF | 1788 bp |
| Locus ID | 4771 |
| UniProt ID | P35240 |
| Domains | B41, ERM |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longer predominant isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
15?-methoxypuupehenol induces antitumor effects in vitro and in vivo against human glioblastoma and breast cancer models
,Hilliard, T;Miklossy, G;Chock, C;Yue, P;Williams, P;Turkson, J;,
Mol. Cancer Ther
,PubMed ID 28069875
[NF2]
|
|
Co-targeting of FAK and MDM2 triggers additive anti-proliferative effects in mesothelioma via a coordinated reactivation of p53
,Ou, WB;Lu, M;Eilers, G;Li, H;Ding, J;Meng, X;Wu, Y;He, Q;Sheng, Q;Zhou, HM;Fletcher, JA;,
Br. J. Cancer
,PubMed ID 27736841
[NF2]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205883 | NF2 (Myc-DDK-tagged)-Human neurofibromin 2 (merlin) (NF2), transcript variant 1 |
CNY 6,648.00 |
|
| RC205883L1 | Lenti ORF clone of Human neurofibromin 2 (merlin) (NF2), transcript variant 1, Myc-DDK-tagged |
CNY 9,048.00 |
|
| RC205883L2 | Lenti ORF clone of Human neurofibromin 2 (merlin) (NF2), transcript variant 1, mGFP tagged |
CNY 9,048.00 |
|
| RC205883L3 | Lenti ORF clone of Human neurofibromin 2 (merlin) (NF2), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC205883L4 | Lenti ORF clone of Human neurofibromin 2 (merlin) (NF2), transcript variant 1, mGFP tagged |
CNY 9,048.00 |
|
| RG205883 | NF2 (tGFP-tagged) - Human neurofibromin 2 (merlin) (NF2), transcript variant 1 |
CNY 8,248.00 |
