LIM Kinase 1 (LIMK1) (NM_002314) Human Untagged Clone

CAT#: SC123883

LIMK1 (untagged)-Human LIM domain kinase 1 (LIMK1), transcript variant 1



  "NM_002314" in other vectors (6)

CNY 4,824.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
LIMK1 (LIM Kinase 1 ) mouse monoclonal antibody, clone OTI6B4 (formerly 6B4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms LIMK; LIMK-1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_002314 edited
GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAAT
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCGCCTCGGTCCG
CCCCTCCGCTCGCTCCCCAAGCCGCCGCGGCGCCGAGCCGGTTTCCCCGCCGGTGTCCGA
GAGGCGCCCCCGGCCCGGCCGCCCCCAGCCCCAGCCCCGCCGGGCCCCGCCCCCCGTCGA
GTGCATGAGGTTGACGCTACTTTGTTGCACCTGGAGGGAAGAACGTATGGGAGAGGAAGG
AAGCGAGTTGCCCGTGTGTGCAAGCTGCGGCCAGAGGATCTATGATGGCCAGTACCTCCA
GGCCCTGAACGCGGACTGGCACGCAGACTGCTTCAGGTGTTGTGACTGCAGTGCCTCCCT
GTCGCACCAGTACTATGAGAAGGATGGGCAGCTCTTCTGCAAGAAGGACTACTGGGCCCG
CTATGGCGAGTCCTGCCATGGGTGCTCTGAGCAAATCACCAAGGGACTGGTTATGGTGGC
TGGGGAGCTGAAGTACCACCCCGAGTGTTTCATCTGCCTCACGTGTGGGACCTTTATCGG
TGACGGGGACACCTACACGCTGGTGGAGCACTCCAAGCTGTACTGCGGGCACTGCTACTA
CCAGACTGTGGTGACCCCCGTCATCGAGCAGATCCTGCCTGACTCCCCTGGCTCCCACCT
GCCCCACACCGTCACCCTGGTGTCCATCCCAGCCTCATCTCATGGCAAGCGTGGACTTTC
AGTCTCCATTGACCCCCCGCACGGCCCACCGGGCTGTGGCACCGAGCACTCACACACCGT
CCGCGTCCAGGGAGTGGATCCGGGCTGCATGAGCCCAGATGTGAAGAATTCCATCCACGT
CGGAGACCGGATCTTGGAAATCAATGGCACGCCCATCCGAAATGTGCCCCTGGACGAGAT
TGACCTGCTGATTCAGGAAACCAGCCGCCTGCTCCAGCTGACCCTCGAGCATGACCCTCA
CGATACACTGGGCCACGGGCTGGGGCCTGAGACCAGCCCCCTGAGCTCTCCGGCTTATAC
TCCCAGCGGGGAGGCGGGCAGCTCTGCCCGGCAGAAACCTGTCTTGAGGAGCTGCAGCAT
CGACAGGTCTCCGGGCGCTGGCTCACTGGGCTCCCCGGCCTCCCAGCGCAAGGACCTGGG
TCGCTCTGAGTCCCTCCGCGTAGTCTGCCGGCCACACCGCATCTTCCGGCCGTCGGACCT
CATCCACGGGGAGGTGCTGGGCAAGGGCTGCTTCGGCCAGGCTATCAAGGTGACACACCG
TGAGACAGGTGAGGTGATGGTGATGAAGGAGCTGATCCGGTTCGACGAGGAGACCCAGAG
GACGTTCCTCAAGGAGGTGAAGGTCATGCGATGCCTGGAACACCCCAACGTGCTCAAGTT
CATCGGGGTGCTCTACAAGGACAAGAGGCTCAACTTCATCACTGAGTACATCAAGGGCGG
CACGCTCCGGGGCATCATCAAGAGCATGGACAGCCAGTACCCATGGAGCCAGAGAGTGAG
CTTTGCCAAGGACATCGCATCAGGGATGGCCTACCTCCACTCCATGAACATCATCCACCG
AGACCTCAACTCCCACAACTGCCTGGTCCGCGAGAACAAGAATGTGGTGGTGGCTGACTT
CGGGCTGGCGCGTCTCATGGTGGACGAGAAGACTCAGCCTGAGGGCCTGCGGAGCCTCAA
GAAGCCAGACCGCAAGAAGCGCTACACCGTGGTGGGCAACCCCTACTGGATGGCACCTGA
GATGATCAACGGCCGCAGCTATGATGAGAAGGTGGATGTGTTCTCCTTTGGGATCGTCCT
GTGCGAGATCATCGGGCGGGTGAACGCAGACCCTGACTACCTGCCCCGCACCATGGACTT
TGGCCTCAACGTGCGAGGATTCCTGGACCGCTACTGCCCCCCAAACTGCCCCCCGAGCTT
CTTTCCCATCACCGTGCGCTGTTGCGATCTGGACCCCGAGAAGAGGCCATCCTTTGTGAA
GCTGGAACACTGGCTGGAGACCCTCCGCATGCACCTGGCCGGCCACCTGCCACTGGGCCC
ACAGCTGGAGCAGCTGGACAGAGGTTTCTGGGAGACCTACCGGCGCGGCGAGAGCGGACT
GCCTGCCCACCCTGAGGTCCCCGACTGAGCCAGGGCCACTCAGCTGCCCCTGTCCCCACC
TCTGGAGAATCCACCCCCACCAGATTCCTCCGCGGGAGGTGGCCCTCAGCTGGGACAGTG
GGGACCCAGGCTTCTCCTCAGAGCCAGGCCCTGACTTGCCTTCTCCCACCCCGTGGACCG
CTTCCCCTGCCTTCTCTCTGCCGTGGCCCAGAGCCGGCCCAGCTGCACACACACACCATG
CTCTCGCCCTGCTGTAACCTCTGTCTTGGCAGGGCTGTCCCCTCTTGCTTCTCCTTGCAT
GAGCTGGAGGGCCTGTGTGAGTTACGCCCCTTTCCACACGCCGCTGCCCCAGCAACCCTG
TTCACGCTCCACCTGTCTGGTCCATAGCTCCCTGGAGGCTGGGCCAGGAGGCAGCCTCCG
AACCATGCCCCATATAACGCTTGGGTGCGTGGGAGGGCGCACATCAGGGCAGAGGCCAAG
TTCCAGGTGTCTGTGTTCCCAGGAACCAAATGGGGAGTCTGGGGCCCGTTTTCCCCCCAG
GGGGTGTCTAGGTAGCAACAGGTATCGAGGACTCTCCAAACCCCCAAAGCAGAGAGAGGG
CTGATCCCATGGGGCGGAGGTCCCCAGTGGCTGAGCAAACAGCCCCTTCTCTCGCTTTGG
GTCTTTTTTTTGTTTCTTTCTTAAAGCCACTTTAGTGAGAAGCAGGTACCAAGCCTCAGG
GTGAAGGGGGTCCCTTGAGGGAGCGTGGAGCTGCGGTGCCCTGGCCGGCGATGGGGAGGA
GCCGGCTCCGGCAGTGAGAGGATAGGCACAGTGGACCGGGCAGGTGTCCACCAGCAGCTC
AGCCCCTGCAGTCATCTCAGAGCCCCTTCCCGGGCCTCTCCCCCAAGGCTCCCTGCCCCT
CCTCATGCCCCTCTGTCCTCTGCGTTTTTTCTGTGTAATCTATTTTTTAAGAAGAGTTTG
TATTATTTTTTCATACGGCTGCAGCAGCAGCTGCCAGGGGCTTGGGATTTTATTTTTGTG
GCGGGCGGGGGTGGGAGGGCCATTTTGTCACTTTGCCTCAGTTGAGCATCTAGGAAGTAT
TAAAACTGTGAAGCTTTCTCAGTGCACTTTGAACCTGGAAAACAATCCCAACAGGCCCGT
GGGACCATGACTTAGGGAGGTGGGACCCACCCACCCCCATCCAGGAACCGTGACGTCCAA
GGAACCAAACCCAGACGCAGAACAATAAAATAAATTCCGTACTCCCCACCCAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAA
>OriGene 5' read for NM_002314 unedited
ATAGGGCGGCCGCGAATTCGGCACGAGGCCCGCCTCGGTCCGCCCCTCCGCTCGCTCCCC
AAGCTCGCCGCGGCGCCGAGCCGGTTTCCCCGCCGGTGTCCGAGAGGCGCCCCCGGCCCG
GCCGCCCCCAGCCCCAGCCCCGCCGGGCCCCGCCCCCCGTCGAGTGCATGAGGTTGACGC
TACTTTGTTGCACCTGGAGGGAAGAACGTATGGGAGAGGAAGGAAGCGAGTTGCCCGTGT
GTGCAAGCTGCGGCCAGAGGATCTATGATGGCCAGTACCTCCAGGCCCTGAACGCGGACT
GGCACGCAGACTGCTTCAGGTGTTGTGACTGCAGTGCCTCCCTGTCGCACCAGTACTATG
AGAAGGATGGGCAGCTCTTCTGCAAGAAGGACTACTGGGCCCGCTATGGCGAGTCCTGCC
ATGGGTGCTCTGAGCAAATCACCAAGGGACTGGTTATGGTGGCTGGGGAGCTGAAGTACC
ACCCCGAGTGTTTCATCTGCCTCACGTGTGGGACCTTTATCGGTGACGGGGACACCTACA
CGCTGGTGGAGCACTCCAAGCTGTACTGCGGGCACTGCTACTACCAGACTGTGGTGACCC
CCGTCATCGAGCAGATCCTGCCTGACTCCCCTGGCTCCCAGCTGCCCCACACCGTCACCC
TGGTGTCCATCCCAGCCTCATCTCATGGCAAGCGTGGACTTTCAGTCTCCATTGACCCCC
CGCACGGCCCACCGGGCTGTGGN
Restriction Sites NotI-NotI     
ACCN NM_002314
Insert Size 3500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002314.2, NP_002305.1
RefSeq Size 3332 bp
RefSeq ORF 1944 bp
Locus ID 3984
UniProt ID P53667
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
Gene Summary There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The exon structure of the 5' UTR has been inferred from cross-species comparisons and lacks direct experimental support.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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