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CYP7A1 (NM_000780) Human Untagged Clone
CAT＃: SC123882
CYP7A1 (untagged)-Human cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1)
CNY 3,760.00
CNY 7,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CP7A; CYP7; CYPVII |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000780 edited
CGGCCGCGAATTCGGCACCAGGCTTTGTCAACCAAGCTCAAGTTAATGGATCTGGATACT ATGTATATAAAAAGCCTAGCTTGAGTCTCTTTTCAGTGGCATCCTTCCCTTTCTAATCAG AGATTTTCTTCCTCAGAGATTTTGGCCTAGATTTGCAAAATGATGACCACATCTTTGATT TGGGGGATTGCTATAGCAGCATGCTGTTGTCTATGGCTTATTCTTGGAATTAGGAGAAGG CAAACGGGTGAACCACCTCTAGAGAATGGATTAATTCCATACCTGGGCTGTGCTCTGCAA TTTGGTGCCAATCCTCTTGAGTTCCTCAGAGCAAATCAAAGGAAACATGGTCATGTTTTT ACCTGCAAACTAATGGGAAAATATGTCCATTTCATCACAAATCCCTTGTCATACCATAAG GTGTTGTGCCACGGAAAATATTTTGATTGGAAAAAATTTCACTTTGCTACTTCTGCGAAG GCATTTGGGCACAGAAGCATTGACCCGATGGATGGAAATACCACTGAAAACATAAACGAC ACTTTCATCAAAACCCTGCAGGGCCATGCCTTGAATTCCCTCACGGAAAGCATGATGGAA AACCTCCAACGTATCATGAGACCTCCAGTCTCCTCTAACTCAAAGACCGCTGCCTGGGTG ACAGAAGGGATGTATTCTTTCTGCTACCGAGTGATGTTTGAAGCTGGGTATTTAACTATC TTTGGCAGAGATCTTACAAGGCGGGACACACAGAAAGCACATATTCTAAACAATCTTGAC AACTTCAAGCAATTCGACAAAGTCTTTCCAGCCCTGGTAGCAGGCCTCCCCATTCACATG TTCAGGACTGCGCACAATGCCCGGGAGAAACTGGCAGAGAGCTTGAGGCACGAGAACCTC CAAAAGAGGGAAAGCATCTCAGAACTGATCAGCCTGCGCATGTTTCTCAATGACACTTTG TCCACCTTTGATGATCTGGAGAAGGCCAAGACACACCTCGTGGTCCTCTGGGCATCGCAA GCAAACACCATTCCAGCGACTTTCTGGAGTTTATTTCAAATGATTAGGAACCCAGAAGCA ATGAAAGCAGCTACTGAAGAAGTGAAAAGAACATTAGAGAATGCTGGTCAAAAAGTCAGC TTGGAAGGCAATCCTATTTGTTTGAGTCAAGCAGAACTGAATGACCTGCCAGTATTAGAT AGTATAATCAAGGAATCGCTGAGGCTTTCCAGTGCCTCCCTCAACATCCGGACAGCTAAG GAGGATTTCACTTTGCACCTTGAGGACGGTTCCTACAACATCCGAAAAGATGACATCATA GCTCTTTACCCACAGTTAATGCACTTAGATCCAGAAATCTACCCAGACCCTTTGACTTTT AAATATGATAGGTATCTTGATGAAAACGGGAAGACAAAGACTACCTTCTATTGTAATGGA CTCAAGTTAAAGTATTACTACATGCCCTTTGGATCGGGAGCTACAATATGTCCTGGAAGA TTGTTCGCTATCCACGAAATCAAGCAATTTTTGATTCTGATGCTTTCTTATTTTGAATTG GAGCTTATAGAGGGCCAAGCTAAATGTCCACCTTTGGACCAGTCCCGGGCAGGCTTGGGC ATTTTGCCGCCATTGAATGATATTGAATTTAAATATAAATTCAAGCATTTGTGAATACAT GGCTGGAATAAGAGGACACTAGATGATATTACAGGACTGCAGAACACCCTCACCACACAG TCCCTTTGGACAAATGCATTTAGTGGTGGTAGAAATGATTCACCAGGTCCAATGTTGTTC ACCAGTGCTTGCTTGTGAATCTTAACATTTTGGTGACAGTTTCCAGATGCTATCACAGAC TCTGCTAGTGAAAAGAACTAGTTTCTAGGAGCACAATAATTTGTTTTCATTTGTATAAGT CCATGAATGTTCATATAGCCAGGGATTGAAGTTTATTATTTTCAAAGGAAAACACCTTTA TTTTATTTTTTTTCAAAATGAAGATACACATTACAGCCAGGTGTGGTAGCAGGCACCTGT AGTCTTAGCTACTCGAGAGGCCA >OriGene 5' read for NM_000780 unedited
CCGTCAGATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGGCTTTGTCA ACCAAGCTCAAGTTAATGGATCTGGATACTATGTATATAAAAAGCCTAGCTTGAGTCTCT TTTCAGTGGCATCCTTCCCTTTCTAATCAGAGATTTTCTTCCTCAGAGATTTTGGCCTAG ATTTGCAAAATGATGACCACATCTTTGATTTGGGGGATTGCTATAGCAGCATGCTGTTGT CTATGGCTTATTCTTGGAATTAGGAGAAGGCAAACGGGTGAACCACCTCTAGAGAATGGA TTAATTCCATACCTGGGCTGTGCTCTGCAATTTGGTGCCAATCCTCTTGAGTTCCTCAGA GCAAATCAAAGGAAACATGGTCATGTTTTTACCTGCAAACTAATGGGAAAATATGTCCAT TTCATCACAAATCCCTTGTCATACCATAAGGTGTTGTGCCACGGAAAATATTTTGATTGG AAAAAATTTCACTTTGCTACTTCTGCGAAGGCATTTGGGCACAGAAGCATTGACCCGATG GATGGAAATACCACTGAAAACATAAACGACACTTTCATCAAAACCCTGCAGGGCCATGCC TTGAATTCCCTCACGGAAAGCATGATGGANAACCTCCAACGTATCATGAGACCTCCAGTC TCCTCTAACTCAAAGACCGCTGCCTGGGTGACAGAAGGGATGTATTCTTTCTGCTACCGA GTGATGTTTGAAGCTGGGTATTTAACTATCTTTGGGCAGAGATCTTACAAGGCGGGACAC ACAAGAAGCACATATTCTAAACAATCTTGACAACTTTCAGCAATTCGACAAAGTCTTTCA GCCCTGGTAGCACGCCTCCCCATTTCCCTGTTCAGGACTGCGCACAATGCCCGGGAAGAA CTTGCAGAGAGCTTGGAGGCCCAAGACCN >OriGene 3' read for NM_000780 unedited
GGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTAGATTCATACTTTTATTTCTGAAAGATGAATCATTTCAAAATTATCAAATGTT AGAATATTTTCTTAATATTTGTAAAATAAGCAAAAAGGTAAATATAATTTAAATATAAAT CCTACCCAGAAATATCAATTGACATAGAGCTGATAACTCATACTTAAAATATTTTATCAA AGCATTGTTGTACACAGCATTTAAACACTCGAGTCACCTTAGTTTTTTTCATCTGCAAGT CATTTAGCGATAAAAAGCATTTAAATAAAACATAATGTCAAGCTGCACTTAATACACTCC ACATGTTTCATAACCAAAGCTTCCAAATGGCTATTTCCATCCAGTTTTAACTTTAAAACA TATCACATAAAAGCTACATTTTTTCAGTGATAATGTGTATTTAACAATGACAGAAAAACT ACCTTTTTTAATCTCTGAGAAAACTCTTCTCATTAATGTGAATGAAATAAAAATATAGAG AAGATAGTTAAAGCCGGACATAGAGTTTTCAGCACAATAAGTTAAAACATGAAGGGAAAA TAAAACCACCTTAGTGTTTTTGGAAATCAAGATATCATGTTTAAGCCCTGATTTTTGATA TAGAGAAGTGAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000780 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000780.2, NP_000771.1 |
| RefSeq Size | 2877 bp |
| RefSeq ORF | 1515 bp |
| Locus ID | 1581 |
| UniProt ID | P22680 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, P450, Transmembrane |
| Protein Pathways | Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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STRUCTURAL CHARACTERIZATION OF HUMAN CHOLESTEROL 7a-HYDROXYLASE
,Tempel, W;Grabovec, I;MacKenzie, F;Dichenko, YV;Usanov, SA;Gilep, AA;Park, HW;Strushkevich, N;,
J. Lipid Res.
,PubMed ID 24927729
[CYP7A1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211019 | CYP7A1 (Myc-DDK-tagged)-Human cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) |
CNY 3,752.00 |
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| RC211019L1 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), Myc-DDK-tagged |
CNY 6,152.00 |
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| RC211019L2 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), mGFP tagged |
CNY 5,890.00 |
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| RC211019L3 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211019L4 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), mGFP tagged |
CNY 5,890.00 |
|
| RG211019 | CYP7A1 (tGFP-tagged) - Human cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) |
CNY 5,352.00 |
