GPR3 (BC032702) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACCA; adenylate cyclase constitutive activator; G protein-coupled receptor 3; OTTHUMP00000003475; OTTHUMP00000043209
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC032702 edited CGCGGGGGTTTCTCGGGGTCCACGCACGCCCTGCGCCGCCAGGACCCGAGCGGAGCCTCC CCGCGGCCCGGCCGCGCCTGGTCCTGAGCGGTACCATGATGTGGGGTGCAGGCAGCCCTC TGGCCTGGCTCTCAGCTGGCTCAGGCAACGTGAATGTAAGCAGCGTGGGCCCAGCAGAGG GGCCCACAGGTCCAGCCGCACCACTGCCCTCGCCTAAGGCCTGGGATGTGGTGCTCTGCA TCTCAGGCACCCTGGTGTCCTGCGAGAATGCGCTAGTGGTGGCCATCATCGTGGGCACTC CTGCCTTCCGTGCCCCCATGTTCCTGCTGGTGGGCAGCCTGGCCGTGGCAGACCTGCTGG CAGGCCTGGGCCTGGTCCTGCACTTTGCTGCTGTCTTCTGCATCGGCTCAGCGGAGATGA GCCTGGTGCTGGTTGGCGTGCTGGCAATGGCCTTTACCGCCAGCATCGGCAGTCTACTGG CCATCACTGTCGACCGCTACCTTTCTCTGTACAATGCCCTCACCTACTATTCAGAGACAA CAGTGACACGGACCTATGTGATGCTGGCCTTAGTGTGGGGAGGTGCCCTGGGCCTGGGGC TGCTGCCTGTGCTGGCCTGGAACTGCCTGGATGGCCTGACCACATGTGGCGTGGTTTATC CACTCTCCAAGAACCATCTGGTAGTTCTGGCCATTGCCTTCTTCATGGTGTTTGGCATCA TGCTGCAGCTCTACGCCCAAATCTGCCGCATCGTCTGCCGCCATGCCCAGCAGATTGCCC TTCAGCGGCACCTGCTGCCTGCCTCCCACTATGTGGCCACCCGCAAGGGCATTGCCACAC TGGCCGTGGTGCTTGGAGCCTTTGCCGCCTGCTGGTTGCCCTTCACTGTCTACTGCCTGC TGGGTGATGCCCACTCTCCACCTCTCTACACCTATCTTACCTTGCTCCCTGCCACCTACA ACTCCATGATCAACCCTATCATCTACGCCTTCCGCAACCAGGATGTGCAGAAAGTGCTGT GGGCTGTCTGCTGCTGCTGTTCCTCTTCCAAGATCCCCTTCCGATCCCGCTCCCCCAGTG ATGTCTAGCTGAGTCTTCATGACCCTTCAACCCTGATTACTACAGAATTCCAGAATGTTA GGCTCTCCAGGGCTTCTTTCCAAACCCCCAGCTCCACACCCCCCAGACCCAGCTGGTTCT GGAGTTCTAGGACATTGGGTGTTTCAAGGTTCTGTTCAGATCCCTATGGGGGCCCAGCTG GCTCCACGGTTCCAGAATGTTCAGGTGGTCAGTGTTCTACTCAGAAATGTCTCACAGCCC AGCTGGGTTGCAATTCCAGAATGCTGGGAGTTTTACAGTGCCATTCCAAGTCCCAGATGT CCCTCTTCCCCCAAACTTGACCTTGACCATGTCACTTTACGTTTGAATTTCTGAGCTAAA GAGTCAGAGAGATTAGTCACATAGTTGCCTAAATAGGAGAGAGAAAGATTATATATGCAC ATATACAAAGACAGTGTCTATTTATGATTGATTTATTTATTTATAAATTTACTTATGGGT GGTAAGGGGCAAAAAAGAGGCCCACACCTTGATATCCAGGCCATACCAGGGTATCCCTTG TCCCTTCACCCCCATTTCTGACCTCAGTTCCTGGAGGGGGGAAAGGGTGAAAGAGAAACC ACGTATTTTGTTATTATTTTGGATTATTTTTTATCGAAGAGATCATAGAAACCAGAGCCT TCTCCCCAGGCCTGCCCTCCTCGGGTTTGGAAGGGGAACACACCAGCCTCTGGTTTTTTA TTTTTTTAAGAAGCCATCACCTGAGCAACCAAAAATTCCTCTGCGCTGGGGTCCGACTGC CCTCTGGTGGCCATTTGGGGAAAACTGCAGCCCGGCCAGGCAGCTGGGACCAGAATGCAA CCCCAGCTCCACTCCAGCCTGGCGTCCAGGGCCACAGCCATGGCCTGGGGGCCAAGCCTC ACCCTGCGGTGCCCTAAAGGAGGGGGGGCACGAGCCAACACCCCACCCCTCTGCCAACCG GGGTATGGCCCCCAGTGCATTCCCTGTTCCCGTCTCCAACCCAACTCAATAAAAAATGAT TTTGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032702 unedited GAGCGTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGATTCANATCTGGTACCATG ATGTGGGGTGCAGGCAGCCCTCTGGCCTGGCTCTCAGCTGGCTCAGGCAACGTGAATGTA AGCAGCGTGGGCCCAGCAGAGGGGCCCACAGGTCCAGCCGCACCACTGCCCTCGCCTAAG GCCTGGGATGTGGTGCTCTGCATCTCAGGCACCCTGGTGTCCTGCGAGAATGCGCTAGTG GTGGCCATCATCGTGGGCACTCCTGCCTTCCGTGCCCCCATGTTCCTGCTGGTGGGCAGC CTGGCCGTGGCAGACCTGCTGGCAGGCCTGGGCCTGGTCCTGCACTTTGCTGCTGTCTTC TGCATCGGCTCAGCGGAGATGAGCCTGGTGCTGGTTGGCGTGCTGGCAATGGCCTTTACC GCCAGCATCGGCAGTCTACTGGCCATCACTGTCGACCGCTACCTTTCTCTGTACAATGCC CTCACCTACTATTCAGAGACAACAGTGACACGGACCTATGTGATGCTGGCCTTAGTGTGG GGAGGTGCCCTGGGCCTGGGGCTGCTGCCTGTGCTGGCCTGGAACTGCCTGGATGGCCTG ACCACATGTGGCGTGGTTTATCCACTCTCCAAGAACCATCTGGTAGTTCTGGCCATTGCC TTCTTCATGGTGTTTGGCATCATGCTGCAGCTCTACGCCCAAATTTGCCGCATCCTCTGC CGCCATGCCCAGCAGATTGCCCCTTAGCGGCACCTGCTGGCTGCCTCCCATATGTGGCCA CCGCAAGGGATTGCCCACTGGCCGTGGTGCTTTGAGCCTTTGCCCACTGTGGTTGCCCTT CAATGTCTATGGCTGGGGGGGGAAGCCCACTCCCCCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC032702 unedited GNGANGAGCACTGGGGNAGGGTCACAGGTATGCCACCCGGGGTCTGTTCAGGAAACAGCT ATGACCGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTGTCATGAAGACTCAG CTAGACATCACTGGGGGAGCGGGATCGGAAGGGGATCTTGGAAGAGGAACAGCAGCAGCA GACAGCCCACAGCACTTTCTGCACATCCTGGTTGCGGAAGGCGTAGATGATAGGGTTGAT CATGGAGTTGTAGGTGGCAGGGAGCAAGGTAAGATAGGTGTAGAGAGGTGGAGAGTGGGC ATCACCCAGCAGGCAGTAGACAGTGAAGGGCAACCAGCAGGCGGCAAAGGCTCCAAGCAC CACGGCCAGTGTGGCAATGCCCTTGCGGGTGGCCACATAGTGGGAGGCAGGCAGCAGGTG CCGCTGAAGGGCAATCTGCTGGGCATGGCGGCAGACGATGCGGCAGATTTGGGCGTAGAG CTGCAGCATGATGCCAAACACCATGAAGAAGGCAATGGCCAGAACTACCAGATGGTTCTT GGAGAGTGGATAAACCACGCCACATGTGGTCAGGCCATCCAGGCAGTTCCAGGCCAGCAC AGGCAGCAGCCCCAGGCCCAGGGCACCTCCCCACACTAAGGCCAGCATCACATAGGTCCG TGTCACTGTTGTCTCTGAATAGTANGTGAGGGCATTGTACANAGAAAGGTAGCGGTCGAC AGTGATGGCCAATAAACTGCCCATGCTGGCGGTAAAAGCCATTGCCAGCACGCCAACCAG CACCAGGCTCATCTCCGCTGAGCCCATGCAGAAAACGGANNAAAATGCAGGACCAGGCCC CAGCCTGCCAACCGGTCG
Restriction Sites	Please inquire
ACCN	BC032702
Insert Size	1050 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032702.1, AAH32702.1
RefSeq Size	2145 bp
RefSeq ORF	990 bp
Locus ID	2827
Protein Families	Druggable Genome, GPCR, Transmembrane
Gene Summary	This gene is a member of the G protein-coupled receptor family and is found in the cell membrane. G protein-coupled receptors, characterized by a seven transmembrane domain motif, are involved in translating outside signals into G protein mediated intracellular effects. The encoded protein activates adenylate cyclase and modulates amyloid-beta production in a mouse model, suggesting that it may play a role in Alzheimer's disease. [provided by RefSeq, Oct 2012]

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