SMUG1 (BC000417) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FDG; HMUDG; MGC104370; single-strand-selective monofunctional uracil-DNA glycosylase 1; single-strand selective monofunctional uracil DNA glycosylase; UNG3
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC000417 edited CGGCTCCCAGGGTCGTCGGGGCTGAGCCGAGAGGGTGGTTAGAATTTCTATATTTTGGGG TCCCTCCCTCCGTATGGGGTAAGACTCAGTGCAGGGAGAGCCCAGCCTATGAAACCGCCT GGGAGGTCTGCAGGATCAAGGCTTTCCAGTCGGCCGGAGCCAGCGGGCGCCCCCGGAGGC TGGAGTGCAATGGCGCGATCTCAGCTCACTGCAGCCTCCGCCTCCCGGGTTCAAGCGATT CTTCTGCCTCAGCCTCCCGAGTAGCAGATTATGAGCTTACAGAAAGCCTGGCCTACATTT TACTCTTTTTGGATTTCTTCCTCATCAAGAGACTGCTGCGGTGCCTGTCATGTGACAGCG GCATGGACATATGCCCCAGGCTTTCCTGCTGGGGTCCATCCATGAGCCTGCAGGTGCCCT CATGGAGCCCCAGCCCTGCCCTGGAAGCTTGGCTGAGAGCTTCCTGGAGGAGGAGCTTCG GCTCAATGCTGAGCTGAGCCAGCTGCAGTTTTCGGAGCCTGTGGGCATCATCTACAATCC CGTGGAGTATGCATGGGAGCCACATCGCAACTACGTGACTCGCTACTGCCAGGGCCCCAA GGAAGTACTCTTCCTGGGCATGAACCCTGGACCTTTTGGCATGGCCCAGACTGGGGTGCC CTTTGGGGAAGTAAGCATGGTCCGGGACTGGTTGGGCATTGTGGGGCCTGTGCTGACCCC TCCCCAAGAGCATCCTAAACGACCAGTGCTGGGACTGGAGTGCCCACAGTCAGAAGGACC AAGACAAAGCATGGGACATGAAATTAAGAGTGAACTTCTTATGGGAGGCTGCAGCTGGAT CAGAGGAAAAATCCAGTGTGACAGAGTGCAAGTCAGAAGACCTGGCTTTTCATCCCAGCT TTGAAACTTGGAACTTTTTGATTGACAAATTAATAAACCTCTCTATGCCTCAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC000417 unedited AGAGTTCANCATTTGTATACGACTTATATAGGCGGCACGCGAATTCGCACGAGGCGGCTC CCATGGTCGTCGGGGCTGAGCCGAGAGGTGGTTAGAATTTCTATATTTTGGGGTCCCTCC CTCCGTATGGGGTAAGACTCAGTGCAGGGAGAGCCCAGCCTATGAAACCGCCTGGGAGGT CTGCAGGATCAAGGCTTTCCAGTCGGCCGGAGCCAGCGGGCGCCCCCGGAGGCTGGAGTG CAATGGCGCGATCTCAGCTCACTGCAGCCTCCGCCTCCCGGGTTCAAGCGATTCTTCTGC CTCAGCCTCCCGAGTAGCAGATTATGAGCTTACAGAAAGCCTGGCCTACATTTTACTCTT TTTGGATTTCTTCCTCATCAAGAGACTGCTGCGGTGCCTGTCATGTGACAGCGGCATGGA CATATGCCCCATGCTTTCCTGCTGGGGTCCATCCATGAGCCTGCAGGTGCCCTCATGGAG CCCCAGCCCTGCCCTGGAAGCTTGGCTGAGAGCTTCCTGGAGGAGGAGCTTCGGCTCAAT GCTGAGCTGAGCCAGCTGCAGTTTTCGGAGCCTGTGGGCATCATCTACAATCCCGTGGAG TATGCATGGGAGCCACATCGCAACTACGTGACTCGCTACTGCCAGGGCCCCAAGGAAGTA CTCTTCCTGGGCATGAACCCTGGACCTTTTGGCATGGCCCANACTGAAGTGCCCTTTGNN GAAGTAAGCATGGTCCNGGACTGGGTGGGCATTGTGGAGCCTGTGCTGACCCCTCCCCAA AGCATCCTATACGACCAGTGCTGGGACTGGAGTGCCCACAGTCAGAAGGACCCAGACAAA GCTGGGACATGAAATTAGAGTGGACCTCCTATGGAAGCTGCAGCTGGTCAGAGGAAAATC ATGTGACA
Restriction Sites	Please inquire
ACCN	BC000417
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC000417.2, AAH00417.1
RefSeq Size	982 bp
Locus ID	23583
Protein Families	Druggable Genome
Protein Pathways	Base excision repair
Gene Summary	This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011]

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