PPAR alpha (PPARA) (NM_032644) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PPAR, NR1C1, hPPAR, MGC2237, MGC2452
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_032644 edited GAAGTTCAAGATCAAAGTGCCAGCAGATTCAGTGTCATGTGAGGACGTGCTTCCTGCTTC ATAGATAAGAGTGCCACACCCCCCTGTCCCAGTGCACTGGGGCTGTGGATCCCTTCAAAG CTGAGATTGCCTGTCTGTGGTCTCCAGCGTTAAGCACAGTCATTAGCTCAGCTTGGAGCT CGGCGGCACAACCAGCACCATCTGGTCGCGATGGTGGACACGGAAAGCCCACTCTGCCCC CTCTCCCCACTCGAGGCCGGCGATCTAGAGAGCCCGTTATCTGAAGAGTTCCTGCAAGAA ATGGGAAACATCCAAGAGATTTCGCAATCCATCGGCGAGGATAGTTCTGGAAGCTTTGGC TTTACGGAATACCAGTATTTAGGAAGCTGTCCTGGCTCAGATGGCTCGGTCATCACGGAC ACGCTTTCACCAGCTTCGAGCCCCTCCTCGGTGACTTATCCTGTGGTCCCCGGCAGCGTG GACGAGTCTCCCAGTGGAGCATTGAACATCGAATGTAGAATCTGCGGGGACAAGGCCTCA GGCTATCATTACGGAGTCCACGCGTGTGAAGGCTGCAAGGGCTTCTTTCGGCGAACGATT CGACTCAAGCTGGTGTATGACAAGTGCGACCGCAGCTGCAAGATCCAGAAAAAGAACAGA AACAAATGCCAGTATTGTCGATTTCACAAGTGCCTTTCTGTCGGGATGTCACACAACGCG ATTCGTTTTGGACGAATGCCAAGATCTGAGAAAGCAAAACTGAAAGCAGAAATTCTTACC TGTGAACATGACATAGAAGATTCTGAAACTGCAGATCTCAAATCTCTGGCCAAGAGAATC TACGAGGCCTACTTGAAGAACTTCAACATGAACAAGGTCAAAGCCCGGGTCATCCTCTCA GGAAAGGCCAGTAACAATCCAGTAGGTGTTTGCGGCTGTTCTGGGTTCTCTTGGCAACAT GGAACCAGTGTCGTAGAGGACGATTAAGGACACATGTGTTGAATGTTGAGAAAATTATAT TTATCCCACAGTTAAGCAAAGGACAGCGAAGATGGAAACAGTTCATTCTGAGACTCTGAG CTGTAGCTTAACAACAACTCCTTTCTTCTTGCTTGGAGCCACCTCAAAGCTCTTAGCAAC TAAGTTATTATACTGGCTATGTAATTAATACACTTAAAAAAAACCTTAATAGCTTACCAA GTACTAAGATGATTTCTTAGGAGCATTTTTTCTTAAATAGAGATAGGTTCTTGCTCTGTT GCCCAGGCTGGAATGCAGTGGTGCAATCATAGTTCACTGCAGCCTTGAACTCCTGGGCTC AAGCAATCCTCCTGCCTCAGCCTCCCAAGGAGCTGGGACTACAGGTGTGCACCACCACAC CTGGCTATGTTTGATGTTGTTGTTGTTTTGTTTTGTTTTTGTTTTTTGGTAGAGATGAGA TGTTTCCCAGGCTGGTCTCAAACTCCTGGCCTCAAGTGATCTTCCCACCTCGGCCTCCCA AAGCACTGGCATTACAGGTGTGAGTCATGGCACCCAGCATTAACTGGATTTAAAAAAAAA AAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_032644 unedited NAAAGTACAGCATTTGTNATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGNAA GTTCAATATCAAAGTGCCAGCAGATTCAGTGTCATGTGAGGACGTGCTTCCTGCTTCATA GATAAGAGTGCCACACCCCCCTGTCCCAGTGCACTGGGGCTGTGGATCCCTTCAAAGCTG AGATTGCCTGTCTGTGGTCTCCAGCGTTAAGCACAGTCATTAGCTCAGCTTGGAGCTCGG CGGCACAACCAGCACCATCTGGTCGCGATGGTGGACACGGAAAGCCCACTCTGCCCCCTC TCCCCACTCGAGGCCGGCGATCTAGAGAGCCCGTTATCTGAAGAGTTCCTGCAAGAAATG GGAAACATCCAAGAGATTTCGCAATCCATCGGCGAGGATAGTTCTGGAAGCTTTGGCTTT ACGGAATACCAGTATTTAGGAAGCTGTCCTGGCTCAGATGGCTCGGTCATCACGGACACG CTTTCACCAGCTTCGAGCCCCTCCTCGGTGACTTATCCTGTGGTCCCCGGCAGCGTGGAC GAGTCTCCCAGTGGAGCATTGAACATCGAATGTAGAATCTGCGGGGACAAGGCCTCAGGC TATCATTACGGAGTCCACGCGTGTGAAGGCTGCAAGGGCTTCTTTCGGCGAACGATTCGA CTCAAGCTGGTGTATGACAAGTGCGACCGCAGCTGCNAGATCCANGAAAAGAACAGAAAC AAATGCCAGTATTGTCGATTTCACAAGTGCCTTTCTGTCGGGATGTCACACAACGCGATT CGTTTTGGACGAATGCCAAGATCTGAGAAAGCANAACTGAAAGCAGAAATTCTTACCTGT GAACATGACATAGNAAGATTCTGAAACTGCAGATCTCAAATCTCTGGCCAAGAAATCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_032644 unedited CCATGGGGCCATTGGGNGATGTGCAACTTCCCAGGNCCAGNANAGCACTGGGGNAGGGTC ACAGGGCATGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTA GAGTCGAGTTTTTTTTTTTTTTTTTTTTAAATCCAGTTAATGCTGGGTGCCATGACTCAC ACCTGTAATGCCAGTGCTTTGGGAGGCCGAGGTGGGAAGATCACTTGAGGCCAGGAGTTT GAGACCAGCCTGGGAAACATCTTATCTCTACCAAAAAACAAAAACAAAACAAAACAACCA CAACATCAAACATAGCCAGGTGTGGTGGTGCACACCTGTAGTCCCAGCTCCTTGGGAGGC TGAGGCAGGAGGATTGCTTGAGCCCAGGAGTTCAAGGCTGCAGTGAACTATGATTGCACC ACTGCATTCCAGCCTGGGCAACAGAGCAAGAACCTATCTCTATTTAAGAAAAAATGCTCC TAAGAAATCATCTTAGTACTTGGTAAGCTATTAAGGTTTTTTTTAAGTGTATTAATTACA TAGCCAGTATAATAACTTAGTTGCTAAGAGCTTTGAGGTGGCTCCAAGCAAGAAGAAAGG AGTTGTTGTTAAGCTACAGCTCAGAGTCTCAGAATGAACTGTTTCCATCTTCGCTGTCCT TTGCTTAACTGTGGGATAAATATAATTTTCTCAACATTCAACACATGTGTCCTTAATCGT CCTCTACGACACTGGTTCCATGTTGCCAAGAGAACCCAGNAAAGCCGCAAACACCTACTG GATTGNTACTGGCCTTTCCTGAGAGGATGACCCGGGCTTTGACCTTGTTCATGTTGGAAT TCTTCAAGTAGGCCTCGTAGATTTCTTTGGCCAGAGATTTGAGATCTGCAGTTTAGAATT TTCTATG
Restriction Sites	Please inquire
ACCN	NM_032644
Insert Size	1580 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_032644.3, NP_116033.2
RefSeq Size	3370 bp
RefSeq ORF	777 bp
Locus ID	5465
Protein Families	Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
Protein Pathways	Adipocytokine signaling pathway, PPAR signaling pathway
Gene Summary	Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (7) differs in the 5' UTR and continues past a splice site in the 3' coding region which results in an early stop codon, compared to variant 6. The resulting isoform (2) has a distinct and shorter C-terminus when compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.