CEP290 (BC008641) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	3H11Ag; BBS14; centrosomal protein 290kDa; CTCL tumor antigen se2-2; FLJ13615; FLJ21979; JBTS5; JBTS6; KIAA0373; LCA10; MKS4; monoclonal antibody 3H11 antigen; nephrocytsin-6; NPHP6; prostate cancer antigen T21; rd16; SLSN6
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC008641 edited CAGATGGATTCGGATGAAATGAAAAAAATACTTGCAGAAAATAGTAGGAAAATTACTGTT TTGCAAGTGAATGAAAAATCACTTATAAGGCAATATACAACCTTAGTAGAATTGGAGCGA CAACTTAGAAAAGAAAATGAGAAGCAAAAGAATGAATTGTTGTCAATGGAGGCTGAAGTT TGTGAAAAAATTGGGTGTTTGCAAAGATTTAAGGAAATGGCCATTTTCAAGATTGCAGCT CTCCAAAAAGTTGTAGATAATAGTGTTTCTTTGTCTGAACTAGAACTGGCTAATAAACAG TACAATGAACTGACTGCTAAGTACAGGGACATCTTGCAAAAAGATAATATGCTTGTTCAA AGAACAAGTAACTTGGAACACCTGGAGTGTGAAAACATCTCCTTAAAAGAACAAGTGGAG TCTATAAATAAAGAACTGGAGATTACCAAGGAAAAACTTCACACTATTGAACAAGCCTGG GAACAGGAAACTAAATTAGGTAATGAATCTAGCATGGATAAGGCAAAGAAATCAATAACC AACAGTGACATTGTTTCCATTTCAAAAAAAATAACTATGCTGGAAATGAAGGAATTAAAT GAAAGGCAGCGGGCTGAACATTGTCAAAAAATGTATGAACACTTACGGACTTCGTTAAAG CAAATGGAGGAACGTAATTTTGAATTGGAAACCAAATTTGCTGAGGTTTGATATTATAAG TTTTATCATACAATTATAGAATAAAGAATTAGTTTTGGTAGACATTGTATTATTGTTAAG TGGTTTGTCTGGATCTCTGAAATATCTTATTAATATAGTGCCTATGTTTTGTGTAATAAA TAAATAAAAGATTTAAATCTGAATTGTTTAAAAGGAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008641 unedited NNGGTCGGAATTTGTATACGACTCATATAGGGCGGCCGCGATTCCCGGGATATCGTCGAC CCACGCGTCCGCAGATGGATTCGGATGAAATGAAAAAAATACTTGCAGAAAATAGTAGGA AAATTACTGTTTTGCAAGTGAATGAAAAATCACTTATAAGGCAATATACAACCTTAGTAG AATTGGAGCGACAACTTAGAAAAGAAAATGAGAAGCAAAAGAATGAATTGTTGTCAATGG AGGCTGAAGTTTGTGAAAAAATTGGGTGTTTGCAAAGATTTAAGGAAATGGCCATTTTCA AGATTGCAGCTCTCCAAAAAGTTGTAGATAATAGTGTTTCTTTGTCTGAACTAGAACTGG CTAATAAACAGTACAATGAACTGACTGCTAAGTACAGGGACATCTTGCAAAAAGATAATA TGCTTGTTCAAAGAACAAGTAACTTGGAACACCTGGAGTGTGAAAACATCTCCTTAAAAG AACAAGTGGAGTCTATAAATAAAGAACTGGAGATTACCAAGGAAAAACTTCACACTATTG AACAAGCCTGGGAACAGGAAACTAAATTAGGTAATGAATCTAGCATGGATAAGGCAAAGA AATCAATAACCAACAGTGACATTGTTTCCATTTCAAAAAAAATAACTATGCTGGAAATGA AGGAATTAAATGAAAGGCAGCGGGCTGAACATTGTCAAAAAATGTATGAACACTTACGGA CTTCGTTAAAGCAAATGGAGGAACGTAATTTTGAATTGGAAACCAAATTTGCTGAGGTTT GATATTATAAGTTTTATCATACAATTATAGAATAAAGAATTAGTTTTGGTAGACATTGTA TTATTGNTAAAGTGGNTTTTGTCTGGATCTCTGAAATATCTTATTAATATAGTGCCCTAT GTTTTGTGTTATAAATAAATAAAAGATTTAATCTGAA
Restriction Sites	Please inquire
ACCN	BC008641
Insert Size	910 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008641.1, AAH08641.1
RefSeq Size	910 bp
Locus ID	80184
Gene Summary	This gene encodes a protein with 13 putative coiled-coil domains, a region with homology to SMC chromosome segregation ATPases, six KID motifs, three tropomyosin homology domains and an ATP/GTP binding site motif A. The protein is localized to the centrosome and cilia and has sites for N-glycosylation, tyrosine sulfation, phosphorylation, N-myristoylation, and amidation. Mutations in this gene have been associated with Joubert syndrome and nephronophthisis and the presence of antibodies against this protein is associated with several forms of cancer. [provided by RefSeq, Jul 2008]

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