OGDH (BC009580) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	2-oxoglutarate dehydrogenase; AKGDH; E1k; H_DJ0691F11_gi16307008.sp_cds.1; OGDC; oxoglutarate (alpha-ketoglutarate) dehydrogenase (lip; oxoglutarate decarboxylase'; oxoglutarate dehydrogenase (lipoamide); oxoglutarate dehydrogenase (succinyl-transferring)
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009580 edited CCGAGAGTTTGGAGCCCCGGAGTGGGGTGTCGGCGCCTCATTCGGGTGGAGCTGAGCCGG AGACAGGCAGTTGTGAAAAACTTCAGGACAAAAATGTTTCATTTAAGGACTTGTGCTGCT AAGTTGAGGCCATTGACGGCTTCCCAGACTGTTAAGACATTTTCACAAAACAGACCAGCA GCAGCTAGGACATTTCAACAGATTCGGTGCTATTCTGCACCTGTTGCTGCTGAGCCCTTT CTCAGTGGGACTAGTTCGAACTATGTGGAGGAGATGTACTGTGCTTGGCTGGAAAACCCC AAAAGTGTACATAAGTCATGGGACATTTTTTTTCGCAACACGAATGCCGGAGCCCCACCG GGCACTGCCTACCAGAGTCCCCTTCCCCTGAGCCGAGGCTCCCTGGCTGCTGTGGCCCAT GCACAGTCCCTGGTAGAAGCACAGCCCAACGTGGACAAGCTCGTGGAGGACCACCTGGCA GTGCAGTCGCTCATCAGGGCATATCAGATACGAGGGCACCATGTAGCACAGCTGGACCCC CTGGGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTGACATTATCTCATCCACA GACAAACTTGGGTTCTATGGCCTGGATGAGTCTGACCTCGACAAGGTCTTCCACTTGCCC ACCACCACTTTCATCGGGGGACAGGAATCAGCACTTCCTCTGCGGGAGATCATCCGTCGG CTGGAGATGGCCTACTGCCAGCATATTGGGGTGGAGTTCATGTTCATCAATGACCTGGAG CAGTGCCAGTGGATCCGGCAGAAGTTTGAGACCCCTGGGATCATGCAGTTCACAAATGAG GAGAAACGGACCCTGCTGGCCAGGCTTGTGCGGTCCACCAGGTTTGAGGAGTTCCTACAG CGGAAGTGGTCCTCTGAGAAGCGCTTTGGTCTAGAAGGCTGCGAGGTACTGATCCCTGCC CTCAAGACCATCATTGACAAGTCTAGTGAGAATGGCGTGGACTACGTGATCATGGGCATG CCACACAGAGGGCGGCTGAACGTGCTTGCAAATGTCATCAGGAAGGAGCTGGAACAGATC TTCTGTCAATTCGATTCAAAGCTGGAGGCAGCTGATGAGGGCTCCGGAGATGTGAAGTAC CACCTGGGCATGTATCACCGCAGGATCAATCGTGTCACCGACAGGAACATTACCTTGTCC TTGGTGGCCAACCCTTCCCACCTTGAGGCCGCTGACCCCGTGGTGATGGGCAAGACCAAA GCCGAACAGTTTTACTGTGGCGACACTGAAGGGAAAAAGGTAAGGCCCAGAGAGAGGCGT GCAAGGCAGATCGTCAAGGCCCCATGTTCCAGCATGGAGTTCCGCTCACCAACATAACCC AGAGCCCTGGGTGCATCTAGACTTTAAAAAAATATTTAAAGTCGGCCGGGCGCAGTGTCT CACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCAGATCACCTGAGTTCGGGAG TTGGAGACCAGCCTGACCAACATGGAGAAACTCCATCTCTACTAAAAATACAAAATTAGC TGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCG CTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATTACGCCATTGCACTCCAGCCTG GGCCAACAAGAGCGAAACTCTGTCTCAAAGAAAAAAATAAATAAATAAAAAATATATGTA TATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009580 unedited NNCCAGACACATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCCATTACGGCCGG GGCCGAGAGTTTGGAGCCCCGGAGTGGGGTGTCGGCGCCTCATTCGGGTGGAGCTGAGCC GGAGACAGGCAGTTGTGAAAAACTTCAGGACAAAAATGTTTCATTTAAAGACTTGTGCTG CTAAGTTGAGGCCATTGACGGCTTCCCAGACTGTTAAGACATTTTCACAAAACAGACCAG CAGCAGCTAGGACATTTCAACAGATTCGGTGCTATTCTGCACCTGTTGCTGCTGAGCCCT TTCTCAGTGGGACTAGTTCGAACTATGTGGAGGAGATGTACTGTGCTTGGCTGGAAAACC CCAAAAGTGTACATAAGTCATGGGACATTTTTTTTCGCAACACGAATGCCGGAGCCCCAC CGGGCACTGCCTACCAGAGTCCCCTTCCCCTGAGCCGAGGCTCCCTGGCTGCTGTGGCCC ATGCACAGTCCCTGGTAGAAGCACAGCCCAACGTGGACAAGCTCGTGGAGGACCACCTGG CAGTGCAGTCGCTCATCAGGGCATATCAGATACGAGGGCACCATGTAGCACAGCTGGACC CCCTGAGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTGACATTATCTCATCCA CAGACAAACTTGAGTTCTATGGCCTGGATGAGTCTGACCTCGACAGGTCTTCCACTTGCC CACCACCACTTTCATCGGGGGACAGGAATCAGCACTTCCTCTGCGGNAGATCATCCGTCG GCTGGAGATGGCCTACTGCCAGCATATTGAGGGTGGAGTTCATGTTNCATCATGACCCTN AGCANTGCCCATTGGATCCGGCAGAAGTTTGAGACCCCTGGGATCATGCAGTTCACN
Restriction Sites	Please inquire
ACCN	BC009580
Insert Size	1775 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009580.1, AAH09580.1
RefSeq Size	1775 bp
Locus ID	4967
Protein Families	Druggable Genome
Protein Pathways	Citrate cycle (TCA cycle), Lysine degradation, Metabolic pathways, Tryptophan metabolism
Gene Summary	This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.