HLA (BC012106) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CELIAC1; HLA-DQB; IDDM1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC012106 edited ATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCAATTATGTCT TGGAAGAAGGCTTTGCGGATCCCTGGAGGCCTTCGGGTAGCAACTGTGACCTTGATGCTG GCGATGCTGAGCACCCCGGTGGCTGAGGGCAGAGACTCTCCCGAGGATTTCGTGTACCAG TTTAAGGGCATGTGCTACTTCACCAACGGGACGGAGCGCGTGCGTCTTGTGACCAGATAC ATCTATAACCGAGAGGAGTACGCACGCTTCGACAGCGACGTGGGGGTGTATCGGGCGGTG ACGCCGCTGGGGCCGCCTGCCGCCGAGTACTGGAACAGCCAGAAGGAAGTCCTGGAGAGG ACCCGGGCGGAGTTGGACACGGTGTGCAGACACAACTACCAGTTGGAGCTCCGCACGACC TTGCAGCGGCGAGTGGAGCCCACAGTGACCATCTCCCCATCCAGGACAGAGGCCCTCAAC CACCACAACCTGCTGGTCTGCTCAGTGACAGATTTCTATCCAGCCCAGATCAAAGTCCGG TGGTTTCGGAATGACCAGGAGGAGACAACTGGCGTTGTGTCCACCCCCCTTATTAGGAAC GGTGACTGGACCTTCCAGATCCTGGTGATGCTGGAAATGACTCCCCAGCGTGGAGACGTC TACACCTGCCACGTGGAGCACCCCAGCCTCCAGAACCCCATCATCGTGGAGTGGCGGGCT CAGTCTGAATCTGCCCAGAGCAAGATGCTGAGTGGCATTGGAGGCTTCGTGCTGGGGCTG ATCTTCCTCGGGCTGGGCCTTATTATCCATCACAGGAGTCAGAAAGGGCTCCTGCACTGA CTCCTGAGACTATTTTAACTGGGATTGGTTATCACTTTTCTGTAACGCCTGCTTGTCCCT GCCCAGAATTCCCAGCTGCCTGTGTCAGCCTGTCCCCCTGAGATCAGAGTCCTACAGTGG CTGTCACGCAGCCACCAGGTCATCTCCTTTCATCCCCACCTCGAGGCTGATGGCTGTGAC CCTGCTTCCTGCACTTACCCAGAGCCTCTGCCTGTGCACGGCCAGCTGCGTCTACTGAGG CCCCAAGGGGTTTCTGTTTCTATTCTCTCCTCAGACTGCTCAAGAGAAGCACATGAAAAC CAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC012106 unedited NATCTTTGGGTTTTGTAATACGACTTACTATAGGGCGGCACGCGAATTCGCACGAGGATC CATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCAATTATGTCTTGG AAGAAGGCTTTGCGGATCCCTGGAGGCCTTCGGGTAGCAACTGTGACCTTGATGCTGGCG ATGCTGAGCACCCCGGTGGCTGAGGGCAGAGACTCTCCCGAGGATTTCGTGTACCAGTTT AAGGGCATGTGCTACTTCACCAACGGGACGGAGCGCGTGCGTCTTGTGACCAGATACATC TATAACCGAGAGGAGTACGCACGCTTCGACAGCGACGTGGGGGTGTATCGGGCGGTGACG CCGCTGGGGCCGCCTGCCGCCGAGTACTGGAACAGCCAGAAGGAAGTCCTGGAGAGGACC CGGGCGGAGTTGGACACGGTGTGCAGACACAACTACCAGTTGGAGCTCCGCACGACCTTG CAGCGGCGAGTGGAGCCCACAGTGACCATCTCCCCATCCAGGACAGAGGCCCTCAACCAC CACAACCTGCTGGTCTGCTCAGTGACAGATTTCTATCCAGCCCAGATCAAAGTCCGGTGG TTTCGGAATGACCAGGAGGAGACAACTGGCGTTGTGTCCACCCCCCTTATTAGGAACGGT GACTGGACCTTCCAGATCCTGGTGATGCTGGAAATGACTCCCCAGCGTGGAGACGTCTAC ACCTGCCACGTGGAGCACCCCAGCCTCCAGAACCCCATCATCGTGGAGTGGCGGGCTCAG TCTGAATCTGCCCAGAGCAGATGCTGAGTGGCATTGGAGGCTTCGTGCNTGGGCTGATCT TCCTCCGGCTGGGNCTTATTATNCATCACAGGAGTCAGAAAGGGCTNCTGCACTGACTCC TG
Restriction Sites	Please inquire
ACCN	BC012106
Insert Size	1160 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012106.1, AAH12106.1
RefSeq Size	1160 bp
RefSeq ORF	783 bp
Locus ID	3119
Protein Families	Transmembrane
Protein Pathways	Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
Gene Summary	HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

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