Trefoil Factor 3 (TFF3) (BC017859) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HITF; hP1.B; intestinal trefoil factor; ITF; secretory protein; TFI; trefoil factor 3; trefoil factor 3 (intestinal); trefoil factor 3, HITF, human intestinal trefoil factor
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC017859 edited GGAGTCCTGAGCTGCGTCCCGGAGCCCACGGTGGTCATGGCTGCCAGAGCGCTCTGCATG CTGGGGCTGGTCCTGGCCTTGCTGTCCTCCAGCTCTGCTGAGGAGTACGTGGGCCTGTCT GCAAACCAGTGTGCCGTGCCAGCCAAGGACAGGGTGGACTGCGGCTACCCCCATGTCACC CCCAAGGAGTGCAACAACCGGGGCTGCTGCTTTGACTCCAGGATCCCTGGAGTGCCTTGG TGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGG CCGGGGGATGCGAGGCTCGGAGCACCCTTGCCCGGCTGTGATTGCTGCCAGGCACTGTTC ATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTCTGCTGAAAGTTCATATCTGG AGCCTGATGTCTTAACGAATAAAGGTCCCATGCTCCACCCTAAAAAAAAAAAAAAAAAAA AAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017859 unedited NNNNGGTTCCGTCATTATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCCATTA CGGCCGGGGGGAGTCCTGAGCTGCGTCCCGGAGCCCACGGTGGTCATGGCTGCCAGAGCG CTCTGCATGCTGGGGCTGGTCCTGGCCTTGCTGTCCTCCAGCTCTGCTGAGGAGTACGTG GGCCTGTCTGCAAACCAGTGTGCCGTGCCAGCCAAGGACAGGGTGGACTGCGGCTACCCC CATGTCACCCCCAAGGAGTGCAACAACCGGGGCTGCTGCTTTGACTCCAGGATCCCTGGA GTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGC TGCCCCCGGCCGGGGGATGCGAGGCTCGGAGCACCCTTGCCCGGCTGTGATTGCTGCCAG GCACTGTTCATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTCTGCTGAAAGTT CATATCTGGAGCCTGATGTCTTAACGAATAAAGGTCCCATGCTCCACCCTAAAAAAAAAA AAAAAAAAANNNNNNNNNCCTTGTCGGCCGCCTCGGCCCTCGAGTTNNNNNNNNNNNNNN TTTTTTTTTTTTCCGCGTTCATTATCTCATTTAATTCTTACAAAACCACGGCAAGGATTC AACCCCACAACTGCTGTCTAGAAAACCGAGGAAAGTGACCCACCCCGCGGGAGGTCTGAC CGCCCGAGAGCCCCACCCCCAGGCCCGGGAACCACCCCCGTCCAATTTCCCGGGGGATTC TTTTTCGTTTCCCCGCGGCCCGCCCAGGTGAACCTCCCTTCCCCCCTACAAAAGGAGCTC CCAACGGGGAACCACTTCGAGGGCAACCCCGGGGCCCCCCGGGAG
Restriction Sites	Please inquire
ACCN	BC017859
Insert Size	491 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017859.1, AAH17859.1
RefSeq Size	491 bp
Locus ID	7033
Protein Families	Secreted Protein
Gene Summary	Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

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