Factor H (CFH) (BC020687) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CFHL3; complement factor H-related 3; complement factor H related 3; DOWN16; FHR-3; FHR3; HLF4; OTTHUMP00000034107
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC020687 edited AATGAAAGATTTCAAACCCCAAACAGTGCAACTGAAACTTTTGTATTAGCATACTACTGA GAATATCTAACATGTTGTTACTAATCAATGTCGTTCTGACCTTGTGGGTTTCCTGTGCTA ATGGACAAGTGAAACCTTGTGATTTTCCAGACATTAAACATGGAGGTCTATTTCATGAGA ATATGCGTAGACCATACTTTCCAGTAGCTGTAGGAAAATATTACTCCTATTACTGTGATG AACATTTTGAGACTCCTTCAGGAAGTTACTGGGATTACATTCATTGCACACAAAATGGGT GGTCACCAGCAGTACCATGTCTCAGAAAATGTTATTTTCCTTATTTGGAAAATGGATATA ATCAAAATTATGGAAGAAAGTTTGTACAGGGTAACTCTACAGAAGTTGCCTGCCATCCTG GCTACGGTCTTCCAAAAGCGCAGACCACAGTTACATGTACGGAGAAAGGCTGGTCTCCTA CTCCCAGATGCATCCGTGTCAGAACATGCTCAAAATCAGATATAGAAATTGAAAATGGAT TCATTTCCGAATCTTCCTCTATTTATATTTTAAATAAAGAAATACAATATAAATGTAAAC CAGGATATGCAACAGCAGATGGAAATTCTTCATATATGGTTTTTTGCATTTCTTATTAAG TTTTGCTTCAGATTTTTTTTGTCTTTTCTCCTGTTAATTGCCTTCACTCTCTTTCTGTTC TGTACTTTTCTTTGTATATAAGGATTTTTTGAAAAGATTGTATATCCCTGTTAACAAATT GAAATCTCTATTCACTTTAATAGATTTTTACCCCCAGTTAGCATATGGTTAGTGAGAAGT TGCAGGGTAAGAAGAAAACAATGTTCCCTTTCCCAACACTTTTCCTGATTATAGAGAAAA AAGCATGAACTTGTATAATGATCTAGTCCTGTACAGAATGAGAAATATTAATTGCTAGAC TGAGAATGTTTTGGTGGCTAAAGCTAGGAATAACTTTTTAAGACTGAAGAATGATATAAG CTATCAAATCTAACTCAGTTTTCAAATAACAGGTTTGCAGAGACTGGAGAATAAAAAGTA AAAAATTGTTTTATTAATTCCAGTGACTAATTCTACTTCATCAACATACATCATAAATAA AATTTCAAAAATAATTGCTGATATGGTTTGGCTGTGTCCCACCCAATTTTCACCTTGAAT TATATAATCACCATGCATCAAAGGTAGGGCCAGGTGGAGATAATGGAATCATGGGAGCAG TTTCCCCATACCCCACTCATGGTAGTAAATACATCTCATGAGATCTAATGGTTTTATAAA TGAAAGTTCCCCTGGACAAGTTCTCTTGCCTGCCACCATGTAAGATGTACCTTTGCTACT CATTCACCTTCTGTCATGATGGTGAGGCCTTCCCAGCAATGTGGAACTGTGAGTCCATTA AACCTCTTTCCTTTATAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020687 unedited NGCTTGCTATTTATACCACTTATATAGGCGGCCGCGAATTCGGCCATTACGGCCGGGGGA ATGAAAGATTTCAAACCCCAAACAGTGCAACTGAGACTTTTGTATTAGCATACTACTGAG AATATCTAACATGTTGTTACTAATCAATGTCGTTCTGACCTTGTGGGTTTCCTGTGCTAA TGGACAAGTGAAACCTTGTGATTTTCCAGACATTAAACATGGAGGTCTATTTCATGAGAA TATGCGTAGACCATACTTTTTTGTAGCTGTAGGAAAATATTACTCCTATTACTGTGATGA ACATTTTGAGACTCCTTCAGGAAGTTACTGGGATTACATTCATTGCACACAAAATGGGTG GTCACCAGCAGTACCATGTCTCAGAAAATGTTATTTTCCTTATTTGGAAAATGGATATAA TCAAAATTATGGAAGAAAGTTTGTACAGGGTAACTCTACAGAAGTTGCCTGCCATCCTGG CTACGGTCTTCCAAAAGCGCAGACCACAGTTACATGTACGGAGAAAGGCTGGTCTCCTAC TCCCAGATGCATCCGTGTCAGAACATGCTCAAAATCAGATATAGAAATTGAAAATGGATT CATTTCCGAATCTTCCTCTATTTATATTTTAAATAAAGAAATACAATATAAATGTAAACC AGGATATGCAACAGCAGATGGAAATTCTTCATATATGGTTTTTTGCATTTCTTATTAAGT TTTGCTTCAGATTTTTTTTGTCTTTTCTCCTGTGTATTGCCTTCACTCTCTTTCTGTTCT GTACTTTTCTTTGTATATAAGGAGTTTTTGAAAAGATGTATATCCCTGTTAACAAATTGA AATCTCTATTCACTTTAATAGATTTTTACCCCCAGTTAGCATATGGTTAGTGAGAAGTTT GCGGA
Restriction Sites	Please inquire
ACCN	BC020687
Insert Size	1490 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020687.1, AAH20687.1
RefSeq Size	1490 bp
Locus ID	10878
Protein Families	Secreted Protein
Gene Summary	The protein encoded by this gene is a secreted protein, which belongs to the complement factor H-related protein family. It binds to heparin, and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-related macular degeneration, and with an increased risk of atypical hemolytic-uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

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