WFDC3 (BC026014) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dJ447F3.3; OTTHUMP00000196627; protease inhibitor WAP14; WAP14; WAP four-disulfide core domain 3; whey acidic protein 14
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC026014 edited AGCGGAGCTGCGGGGTTACCATGGGAACCGAACCGCCGCGCCTCGCCCAGGACAGTTACA CTTAGAGGCCTTCAAAAGAGGGAGAATGCCCTCCCGATAAGAACCCATGCAAAGAGCTGT GCCAGGGCGATGAATTGTGTCCGGCTGAACAGAAGTGCTGCACCAGGCTGTGGTCGGATC TGCCGAGACATTCCTAAGGGGAGGAAAAGAGATTGCCCTAGGGTTATTCGGAAACAATCC TGTTTGAAAAGGTGCATCACTGATAAGACATGTCCAGGTGTAAAGAAATGCTGCACGCTT GGCTGCAACAAGAGCTGTGTAGTCCCAATCTCTAAACAGAAGCTGGGGCGGGGCGGTGAT TGTCCAAAAGTTCTGGTGGGCCTGTGCATTGTTGGCTGTGTGATGGATGAGAATTGTCAA GCTGGAGAAAAATGTTGCAAGTCAGGCTGTGGCCGCTTCTGTGTCCCACCAGTCCTGCCC CCAAAACTGACCATGAACCCCAACTGGACTGTGAGGTCTGATTCCGAATTAGAGATCCCG GTGCCCTAGCTGTGCTGATTTGTCTGGAGCTTCTTTGGTAATTCTGGAAGCTTTTCCTGG CAGTCAAGAGAGGGTGACATCCTGGGGCTTGTGACATTTCCAGGGGCACTCATGGCCCTC TCTGCTCTGCTTCTCCTCCTGCCGCTGACCAGAGCATGGGAAATAGCCCTGGATTGGGTA GTGGGTGTGTGGTGCTTCTCTTTCCCAATAAAGGCTGGTGCTGAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC026014 unedited TGGGGGGCTTACGGGTTTTGTACCCCTCTTCTCATAGGCGGACGCCTAACTTCGTATAGC ATACATTATACGAAGTTATGGATCATGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCG GAGCTGCGGGGTTACCATGGGAACCGAACCGCCGCGCCTCGCCCAGGACAGTTACACTTA GAGGCCTTCAAAAGAGGGAGAATGCCCTCCCGATAAGAACCCATGCAAAGAGCTGTGCCA GGGCGATGAATTGTGTCCGGCTGAACAGAAGTGCTGCACCAGGCTGTGGTCGGATCTGCC GAGACATTCCTAAGGGGAGGAAAAGAGATTGCCCTAGGGTTATTCGGAAACAATCCTGTT TGAAAAGGTGCATCACTGATAAGACATGTCCAGGTGTAAAGAAATGCTGCACGCTTGGCT GCAACAAGAGCTGTGTAGTCCCAATCTCTAAACAGAAGCTGGGGCGGGGCGGTGATTGTC CAAAAGTTCTGGTGGGCCTGTGCATTGTTGGCTGTGTGATGGATGAGAATTGTCAAGCTG GAGAAAAATGTTGCAAGTCAGGCTGTGGCCGCTTCTGTGTCCCACCAGTCCTGCCCCCAA AACTGACCATGAACCCCAACTGGACTGTGAGGTCTGATTCCGAATTAGAGATCCCGGTGC CCTATCTGTGCTGATTTGTCTGGAGCTTCTTTGGTAATTCTGGAAGCTTTTCCTGGCAGT CAAGAGAGGGTGACATCCTGGGGCTTGTGACATTTCCAGGGGCACTCATGGCCCTCTCTG CTCTGCTTCTCCTCCTGCCGCTGACCAGAGCATGGGAAATAGCCCTGGATTGGGTAGTGG GTGTGTGGTGCTTCTCTTTCCAATAAAAG
Restriction Sites	Please inquire
ACCN	BC026014
Insert Size	778 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026014.1, AAH26014.2
RefSeq Size	778 bp
Locus ID	140686
Protein Families	Secreted Protein
Gene Summary	This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. The encoded protein contains four WFDC domains. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the telomeric cluster. Alternatively spliced transcript variants have been observed but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

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