SLC35A2 (BC035747) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDG2M; CDGX; UDP-Gal-Tr; UGALT; UGAT; UGT; UGT1; UGT2; UGTL
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC035747 edited CGGGCCGGGCAGATGCCAACATGGCAGCGGTTGGGGCTGGTGGTTCCACCGCGGCGCCCG GGCCAGGGGCGGTTTCCGCGGGTGCATTGGAGCCGGGGACCGCCAGTGCGGCTCACAGGC GCCTGAAGTACATATCCCTAGCTGTGCTGGTGGTCCAGAATGCCTCCCTCATCCTCAGCA TCCGCTACGCCCGCACGTTGCCAGGGGACCGCTTCTTTGCCACCACTGCTGTGGTCATGG CGGAAGTGCTCAAAGGTCTCACCTGCCTGCTGCTGCTCTTCGCACAGAAGAGGGGTAACG TGAAGCACCTGGTTCTCTTCCTCCATGAGGCTGTCCTGGTGCAGTATGTGGACACGCTCA AGCTCGCAGTGCCCTCTCTCATCTACACCTTGCAGAATAACCTCCAGTATGTTGCCATCT CTAACCTACCAGCTGCCACTTTCCAGCCTTCCCCGAGGTGCAGCCAAAGCCATAGCCTCT GCCTCTGCCTCCGCCTCCGGGCCCTGCGTTCACCAGCAGCCTCCCGGGCAGCCACCACCA CCGCAGCTGTCTTCCCACCGTGGAGACCTCATCACGGAGCCCTTTCTGCCAAAGTCAGTG CTGGTGAAGTGAGGGCTGGCAGCAATGGGGGGACACAAGGGAGGGGGACTGGGGTGGAGG GTGTTGGGCATCTGCAGGACCCAAGTCGCCACCCTCCGGGGCCTGGCTCCTCTGGGTTTG GGAGATGGTCTTTTCTCCCAGGTCACTGAGACTTCTGGAGGGGTGTGGGACTAGAGCTGG GTGTCACGTGAACCCTTCCTGGTAGGGTGACCCCCTTCCCCTGGAGGGGGTTTTAGAGCT GCCGCCTCTGCTCCCTCTAACCTCTTTGGAGGCAGGGTTGGGGGTATTGTCATTCAAGGC CTTTTTTTTGTCTGCTCCCTCCCCGACCCTGTGCCCTCTTCTGGAGGTTTCTCGTCTGGG AGAGTCCCTCCCAGCAGTCCCTCCACCTCCATAAGGACACACTGGACAAAACTCCCGCAG CTCTTCAGGAATGACCGATGCCTACCTGTGGGGTTCAGTTGCCCATAGTTTGAGGCCTTC TCTCCTCCCTTACCACCGCTCTGGATCATGTTACTAGTTCCGTCTTTTGTGTGGCCTTGG GCCAGCTTCCTTGATACCTTGAAGATGGGCTCCTTGTGAGTCCCCAGGGAGAAAGGGACA AGAGCTAAGATTTTTGCATCAGCCCTTCTGGCAGAAGGTGTGGTAGGGGCCATTTGTTTT TTTTAGTGGACTTGGGATTTGTGGTGTAATCATATCATTAATGATCCAGGGTGTGGGAAA AATGGAGGTCCTTGAAGTGGCTGAATCTCATTGTATTTAAGACACTGTCAGTTGCCAGAT GTAGGCTTATTTTTGGAGATGTCTAGGAGAGGAAAAAGCTACCAATCATACTCTTGATAT CCGTCTGGCTGTGTGAGGCACCCCTACCTCATGGGGGTGTCTTGGGATTGATGAACTGTG GAACCTGCCTCCTGCGCTCCCCAAAGCTTATTAACCCCTTAACTGTATCGGGGCGGGGTG TGTGTGTGCATGGAAGATGCCTGGGCTGTCTTTGCTATATGTAAATAGAGCCATTGGATC TTTATTTTTGATTAATTTGTTCTGATTTTTTGGTTTGTTTTTTAAGGAACTGTAATGAAC AAATGTCAGGATATCCAATGCCAAATAAAGATGTTGTATTTTTTTAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC035747 unedited AGTTAGGATTTGTAAACGACTTATATAGGCGGCCGACGATTCCCGGGATCGGGCCGGGCA GATGCCAACATGGCAGCGGTTGGGGCTGGTGGTTCCACCGCGGCGCCCGGGCCAGGGGCG GTTTCCGCGGGTGCATTGGAGCCGGGGACCGCCAGTGCGGCTCACAGGCGCCTGAAGTAC ATATCCCTAGCTGTGCTGGTGGTCCAGAATGCCTCCCTCATCCTCAGCATCCGCTACGCC CGCACGTTGCCAGGGGACCGCTTCTTTGCCACCACTGCTGTGGTCATGGCGGAAGTGCTC AAAGGTCTCACCTGCCTGCTGCTGCTCTTCGCACAGAAGAGGGGTAACGTGAAGCACCTG GTTCTCTTCCTCCATGAGGCTGTCCTGGTGCAGTATGTGGACACGCTCAAGCTCGCAGTG CCCTCTCTCATCTACACCTTGCAGAATAACCTCCAGTATGTTGCCATCTCTAACCTACCA GCTGCCACTTTCCAGCCTTCCCCGAGGTGCAGCCAAAGCCATAGCCTCTGCCTCTGCCTC CGCCTCCGGGCCCTGCGTTCACCAGCAGCCTCCCGGGCAGCCACCACCACCGCAGCTGTC TTCCCACCGTGGAGACCTCATCACGGAGCCCTTTCTGCCAAAGTCAGTGCTGGTGAAGTG AGGGCTGGCAGCAATGGGGGGACACAAAGGAGGGGGACTGGGGTGGAGGGTGTGGGGCAT CTGCAGGACCCCAGTCGGCACCCTCCGGGGCCTGGCTCCTCTGGGTTTGGGAGATGGTCT TTTCTCCCAGGTCACTGAGACTTCTGGNAGGGTGGTGGGACTAGAGCTGGGTGTCACGTG AACCCTTCCGGTAGGGTGACCCCCCTTCCCTGGAGGGGGGTTTAGAGCTGCCGCTCTGTT CCCTTTAACTCTTTGGAGGCAGGG
Restriction Sites	Please inquire
ACCN	BC035747
Insert Size	1800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC035747.1, AAH35747.1
RefSeq Size	1763 bp
RefSeq ORF	726 bp
Locus ID	7355
Protein Families	Transmembrane
Gene Summary	This gene encodes a member of the nucleotide-sugar transporter family. The encoded protein is a multi-pass membrane protein. It transports UDP-galactose from the cytosol into Golgi vesicles, where it serves as a glycosyl donor for the generation of glycans. Mutations in this gene cause congenital disorder of glycosylation type IIm (CDG2M). Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

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