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Alanine Transaminase (GPT) (NM_005309) Human Untagged Clone
CAT＃: SC122706
GPT (untagged)-Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAT1; ALT1; GPT1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_005309 edited
AGGGCTGCCACTTGGACTCCCTTGCCAGGGGGCGGGCAGGGGCTGGCTCTGCCTGGGGCC AGGCCCATGTTAACCTGTGCCCCGGCCTGGGGGCCAGAGGGGCAGGCCTGCGCACGGGGA GGCTGTTGTGGCAGCGGAACAGAGACGTGCACGAGTCATGGCCTCGAGCACAGGTGACCG GAGCCAGGCGGTGAGGCATGGACTGAGGGCGAAGGTGCTGACGCTGGACGGCATGAACCC GCGTGTGCGGAGAGTGGAGTACGCAGTGCGTGGCCCCATAGTGCAGCGAGCCTTGGAGCT GGAGCAGGAGCTGCGCCAGGGTGTGAAGAAGCCTTTCACCGAGGTCATCCGTGCCAACAT CGGGGACGCACAGGCTATGGGGCAGAGGCCCATCACCTTCCTGCGCCAGGTCTTGGCCCT CTGTGTTAACCCTGATCTTCTGAGCAGCCCCAACTTCCCTGACGATGCCAAGAAAAGGGC GGAGCGCATCTTGCAGGCGTGTGGGGGCCACAGTCTGGGGGCCTACAGCGTCAGCTCCGG CATCCAGCTGATCCGGGAGGACGTGGCGCGGTACATTGAGAGGCGTGACGGAGGCATCCC TGCGGACCCCAACAACGTCTTCCTGTCCACAGGGGCCAGCGATGCCATCGTGACGGTGCT GAAGCTGCTGGTGGCCGGCGAGGGCCACACACGCACGGGTGTGCTCATCCCCATCCCCCA GTACCCACTCTACTCGGCCACGCTGGCAGAGCTGGGCGCAGTGCAGGTGGATTACTACCT GGACGAGGAGCGTGCCTGGGCGCTGGACGTGGCCGAGCTTCACCGTGCACTGGGCCAGGC GCGTGACCACTGCCGCCCTCGTGCGCTCTGTGTCATCAACCCTGGCAACCCCACCGGGCA GGTGCAGACCCGCGAGTGCATCGAGGCCGTGATCCGCTTCGCCTTCGAAGAGCGGCTCTT TCTGCTGGCGGACGAGGTGTACCAGGACAACGTGTACGCCGCGGGTTCGCAGTTCCACTC ATTCAAGAAGGTGCTCATGGAGATGGGGCCGCCCTACGCCGGGCAGCAGGAGCTTGCCTC CTTCCACTCCACCTCCAAGGGCTACATGGGCGAGTGCGGGTTCCGCGGCGGCTATGTGGA GGTGGTGAACATGGACGCTGCAGTGCAGCAGCAGATGCTGAAGCTGATGAGTGTGCGGCT GTGCCCGCCGGTGCCAGGACAGGCCCTGCTGGACCTGGTGGTCAGCCCGCCCGCGCCCAC CGACCCCTCCTTTGCGCAGTTCCAGGCTGAGAAGCAGGCAGTGCTGGCAGAGCTGGCGGC CAAGGCCAAGCTCACCGAGCAGGTCTTCAATGAGGCTCCTGGCATCAGCTGCAACCCAGT GCAGGGCGCCATGTACTCCTTCCCGCGCGTGCAGCTGCCCCCGCGGGCGGTGGAGCGCGC TCAGGAGCTGGGCCTGGCCCCCGATATGTTCTTCTGCCTGCGCCTCCTGGAGGAGACCGG CATCTGCGTGGTGCCAGGGAGCGGCTTTGGGCAGCGGGAAGGCACCTACCACTTCCGGAT GACCATTCTGCCCCCCTTGGAGAAACTGCGGCTGCTGCTGGAGAAGCTGAGCAGGTTCCA TGCCAAGTTCACCCTCGAGTACTCCTGAGCACCCCAGCTGGGGCCAGGCTGGGTCGCCCT GGACTGTGTGCTCAGGAGCCCTGGGAGGCTCTGGAGCCCACTGTACTTGCTCTTGATGCC TGGCGGGGTGGGGTGGGGGGGGTGCTGGGCCCCTGCCTCTCTGCAGGTCCCTAATAAAGC TGTGTGGCAGTCTGACTCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAA >OriGene 5' read for NM_005309 unedited
GGTCAGAATTTTGTATACGACTCATATAGGGCGGCCGCGATTCCCGGGATATCGTCGACC CACGCGTCCGAGGGCTGCCACTTGGACTCCCTTGCCAGGGGGCGGGCAGGGGCTGGCTCT GCCTGGGGCCAGGCCCATGTTAACCTGTGCCCCGGCCTGGGGGCCAGAGGGGCAGGCCTG CGCACGGGGAGGCTGTTGTGGCAGCGGAACAGAGACGTGCACGAGTCATGGCCTCGAGCA CAGGTGACCGGAGCCAGGCGGTGAGGCATGGACTGAGGGCGAAGGTGCTGACGCTGGACG GCATGAACCCGCGTGTGCGGAGAGTGGAGTACGCAGTGCGTGGCCCCATAGTGCAGCGAG CCTTGGAGCTGGAGCAGGAGCTGCGCCAGGGTGTGAAGAAGCCTTTCACCGAGGTCATCC GTGCCAACATCGGGGACGCACAGGCTATGGGGCAGAGGCCCATCACCTTCCTGCGCCAGG TCTTGGCCCTCTGTGTTAACCCTGATCTTCTGAGCAGCCCCAACTTCCCTGACGATGCCA AGAAAAGGGCGGAGCGCATCTTGCAGGCGTGTGGGGGCCACAGTCTGGGGGCCTACAGCG TCAGCTCCGGCATCCAGCTGATCCGGGAGGACGTGGCGCGGTACATTGAGAGGCGTGACG GAGGCATCCCTGCGGACCCCACAACGTCTTCCTGTCCACAGGGGCCAGCGATGCCATCGT GACGGTGCTGAAGCTGCTGGTGGCCGGCGAGGGCCACACACGCCCGGGTGTGCTCATCCC CATCCCCCAGTACCCACTCTACTCGGCCACGCTGGCAGAGCTGGGCGCAGTGCAGGTGGA TTACTACCTGGACAAGGAGCGTGCCTGGGCGCCTGGACTTGGCCGAACTTACCGTGCACT GGGCCAGGCGCGTGG |
| Restriction Sites | Please inquire |
| ACCN | NM_005309 |
| Insert Size | 1871 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005309.1, NP_005300.1 |
| RefSeq Size | 1924 bp |
| RefSeq ORF | 1491 bp |
| Locus ID | 2875 |
| UniProt ID | P24298 |
| Protein Families | Druggable Genome |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Metabolic pathways |
| Gene Summary | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203756 | GPT (Myc-DDK-tagged)-Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT) |
CNY 3,656.00 |
|
| RC203756L3 | Lenti ORF clone of Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC203756L4 | Lenti ORF clone of Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), mGFP tagged |
CNY 5,890.00 |
|
| RG203756 | GPT (tGFP-tagged) - Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT) |
CNY 5,256.00 |
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