AFAF (EQTN) (BC014307) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Acr formation associated factor; Acrosome formation associated factor; Afaf; chromosome 9 open reading frame 11; OTTHUMP00000022786
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC014307 edited GGGAGACACAGGACCTGCTGGGCCACAGAAAGGAGGCTCTGGGTAGACGCACTAGATTAC TGGATAAATCACTTCAATTTCCCAATGAATTTTATATTGTTTATTTTTATACCTGGAGTT TTTTCCTTAAAAAGTAGCACTTTGAAGCCTACTATTGAAGCATTGCCTAATGTGCTACCT TTAAATGAAGACGTTAATAAGCAGGAAGAAAAGAATGAAGATCATACTCCCAATTATGCT CCTGCTAATGAGAAAAATGGCAATTATTATAAAGATATAAAACAATATGTGTTCACAACA CAAAATCCAAATGGCACTGAGTCTGAAATATCTGTGAGAGCCACAACTGACCTGAATTTT GCTCTAAAAAACGATAAAACTGTCAATGCAACTACATATGAAAAATCCACCATTGAAGAA GAAACAACTACTAGCGAACCCTCTCATAAAAATATTCAAAGTATTTAAATTACTACACTG TATTCATACCTGGAGAATATCATTATGTGACTTAAAAATTTAAGTTTCACTACTCTGAGC ATTACATAAAGCCATAGCATGCTTAAAGAATTTATACTGCTACCTTTTAAAGTCACTAAA ATCTTTTAAATGGTGAATGTCTAAGAGTCAGTCTTCAGGTATACCTGACAAAGGATGAGA AATGAGCTTTTGTAGAAATAGAACTTTATTTTAATAAAAATGTGTTACATTTGTGTCTAA AAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC014307 unedited GCAGTCAGATTTGTATACACTATTAGGCGGCCGCGAATCGCCATTACGCCGGGAGACACA GACCTGCTGGGCCACAGAAGGAGGCTCTGGGTAGACGCACTAGATTACTGGATAAATCAC TTCAATTTCCCAATGAATTTTATATTGTTTATTTTTATACCTGGAGTTTTTTCCTTAAAA AGTAGCACTTTGAAGCCTACTATTGAAGCATTGCCTAATGTGCTACCTTTAAATGAAGAC GTTAATAAGCAGGAAGAAAAGAATGAAGATCATACTCCCAATTATGCTCCTGCTAATGAG AAAAATGGCAATTATTATAAAGATATAAAACAATATGTGTTCACAACACAAAATCCAAAT GGCACTGAGTCTGAAATATCTGTGAGAGCCACAACTGACCTGAATTTTGCTCTAAAAAAC GATAAAACTGTCAATGCAACTACATATGAAAAATCCACCATTGAAGAAGAAACAACTACT AGCGAACCCTCTCATAAAAATATTCAAAGTATTTAAATTACTACACTGTATTCATACCTG GAGAATATCATTATGTGACTTANAAATTTAAGTTTCACTACTCTGAGCATTACATAAAGC CATAGCATGCTTAAAGAATTTATACTGCTACCTTTTTAAGTCACTAAAATCTTTTTAATG GTGAATGTCTAAGAATCAGTCTTCAGGTATACCTGACAAGGATGAGAAATGAGCTTTTGT AGAATAGACTTTATTTTATAAAAAGTGTTACATTGTGTCTAAAAAAAAAAAAAAGNANGN GGNCTTGTCGGCCCCCTCGCCCCCAGTTAANTAAACAGAGAAGATGAGTATTGTGCTGAG AGTACGTTTCTATGTAGACAGAAAAAT
Restriction Sites	Please inquire
ACCN	BC014307
Insert Size	746 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC014307.1, AAH14307.1
RefSeq Size	746 bp
RefSeq ORF	384 bp
Locus ID	54586
Protein Families	Transmembrane
Gene Summary	Acrosomal membrane-anchored protein involved in the process of fertilization and in acrosome biogenesis.[UniProtKB/Swiss-Prot Function]

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