EBAG9 (BC017729) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	cancer associated surface antigen; EB9; estrogen receptor binding site associated, antigen, 9; estrogen receptor binding site associated antigen 9; PDAF; RCAS1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC017729 edited CAGGCTGCTACGGAGCGCGCGCCCGGCTTTGAATGAGCGGGGCTGGGAGTGAGCGGGCGG AGCGCGAGCTCGAGGAAGAGACAGGCAGCGCGCGTGAGCGCGCCTTGTGTGCGCGCGCGG CCCGCGGCAGCTCGGAGCCTCCGCCGGGCGGGCGGGGAGGGGGAGGGGCAGGTTTTGATT CCCACCATGGCCATCACCCAGTTTCGGTTATTTAAATTTTGTACCTGCCTAGCAACAGTA TTCTCATTCCTAAAGAGATTAATATGCAGATCTGGCAGAGGACGGAAATTAAGTGGAGAC CAAATAACTTTGCCAACTACAGTTGATTATTCATCAGTTCCTAAGCAGACAGATGTTGAA GAGTGGACTTCCTGGGATGAAGATGCACCCACCAGTGTAAAGATCGAAGGAGGGAATGGG AATGTGGCAACACAACAAAATTCTTTGGAACAACTGGAACCTGACTATTTTAAGGACATG ACACCAACTATTAGGAAAACTCAGAAAATTGTTATTAAGAAGAGAGAACCATTGAATTTT GGCATCCCAGATGGGAGCACAGGTTTCTCTAGTAGATTAGCAGCTACACAAGATCTGCCT TTTATTCATCAGTCTTCTGAATTAGGTGACTTAGATACCTGGCAGGAAAATACCAATGCA TGGGAAGAAGAAGAAGATGCAGCCTGGCAAGCAGAAGAAGTTCTGAGACAGCAGAAACTA GCAGACAGAGAAAAGAGAGCAGCCGAACAACAAAGGAAGAAAATGGAAAAGGAAGCACAA CGGCTAATGAAGAAGGAACAAAACAAAATTGGTGTGAAACTTTCATAACACATGTTCAAA TTTTATCATGCCAGTAGGAGAAATCTCAGCTCCACAACCCAAGCAACATTTGTATGGATT TAAGAGTATTTTAAGAAGACATACTGCTTGATTTTAATACATTGATCAGGCCATCCAGGA CACCACGATTCTCCCAAAGTACCTTGAACTCTTAGTGATTGAGACTCAAAAAAACAAAAA AGACTTGAGACAATGTTTTCTTCAACATGCTCCAAATATAAGACATTTGTTTGCTGTACA GAAAGTATCACAAATGGAATATATCAGTACCTCTCAAGCTAGTGTTTCTAGCTAAATAAA TGGGTGTATATAATTTTATGGTGGAAAAGAACTGTACTGTCTGTTATGATTTCCTTCAAT GTGCATAATGATAAAATAAATAATTTTAATATTCAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017729 unedited CCCGTTTGGATTTGNATACGACTTATATAGGGCGGCCGCGATTCCCGGGATATCGTCGAC CCACGCGTCCGCAGGCTGCTACGGAGCGCGCGCCCGGCTTTGAATGAGCGGGGCTGGGAG TGAGCGGGCGGAGCGCGAGCTCGAGGAAGAGACAGGCAGCGCGCGTGAGCGCGCCTTGTG TGCGCGCGCGGCCCGCGGCAGCTCGGAGCCTCCGCCGGGCGGGCGGGGAGGGGGAGGGGC AGGTTTTGATTCCCACCATGGCCATCACCCAGTTTCGGTTATTTAAATTTTGTACCTGCC TAGCAACAGTATTCTCATTCCTAAAGAGATTAATATGCAGATCTGGCAGAGGACGGAAAT TAAGTGGAGACCAAATAACTTTGCCAACTACAGTTGATTATTCATCAGTTCCTAAGCAGA CAGATGTTGAAGAGTGGACTTCCTGGGATGAAGATGCACCCACCAGTGTAAAGATCGAAG GAGGGAATGGGAATGTGGCAACACAACAAAATTCTTTGGAACAACTGGAACCTGACTATT TTAAGGACATGACACCAACTATTAGGAAAACTCAGAAAATTGTTATTAAGAAGAGAGAAC CATTGAATTTTGGCATCCCAGATGGGAGCACAGGTTTCTCTAGTAGATTAGCAGCTACAC AAGATCTGCCTTTTATTCATCAGTCTTCTGAATTAGGTGACTTAGATACCTGGCAGGAAA ATACCAATGCATGGGAAAGAGAAGAAGATGCAGCCTGGCAAGCAGAAGAAGTTCTGAGAC AGCNGAAACTAGCAGACAGAGAAAAGAGAGCAGCCGAAACACANAGGAAGAAAATGGAAA GGGAGCACAACGGCTAATGGAGAANGGGACANACANAATTGTGTGAAACTTTCATACACA TGTTA
Restriction Sites	Please inquire
ACCN	BC017729
Insert Size	1252 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017729.1, AAH17729.1
RefSeq Size	1252 bp
Locus ID	9166
Protein Families	Druggable Genome
Gene Summary	This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]

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