SPAG8 (BC019247) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BS-84; HSD-1; hSMP-1; MGC26201; OTTHUMP00000021352; SMP1; SPAG3; sperm associated antigen 8; sperm membrane protein 1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC019247 edited GTCGAGAGCGGAGTCTGAAGATGGAGACCAACGGGTCTACGGAGGGATCGCGGTCGCGGT CGCGATCTTTAGACATACAGCCCAGCTCCGAAGGACTGGGGCCCACTTCGGAACCGTTTC CTTCTTCAGATGACAGTCCCAGGTCGGCCCTGGCAGCTGCAACCGCAGCAGCTGCAGCGG CTGCATCAGCTGCTGCAGCTACTGCAGCCTTCACCACTGCCAAAGCAGCTGCATTATCTA CAAAGACCCCAGCGCCCTGTTCTGAGTTCATGGAGCCGTCCTCTGACCCCAGCCTTCTTG GGGAGCCCTGTGCGGGACCCGGCTTTACCCACAATATAGCCCATGGGAGTCTTGGCTTTG AGCCCGTCTATGTTTCCTGTATTGCTCAGGACACTTGCACTACAACTGACCATAGTTCTA ATCCTGGCCCTGTTCCAGGCTCTAGCTCTGGGCCTGTTCTTGGTTCCAGCTCAGGTGCTG GCCATGGCTCTGGCTCTGGCTCTGGTCCTGGCTGTGGCTCTGTCCCTGGCTCTGGCTCTG GTCCTGGTCCTGGCTCTGGTCCTGGCTCTGGTCCTGGTCATGGCTCTGGCTCTCATCCTG GTCCTGCCTCTGGGCCTGGTCCAGACACTGGCCCTGACTCTGAGCTCAGCCCCTGTATTC CTCCAGGGTTCAGAAACCTGGTGGCAGATCGGGTCCTTAACTATACCTCCTGGAGTCAGC ACTGCCCCTGGGAGCCCCAGAAACAACCACCTTGGGAATTTTTGCAAGTCTTAGAACCGG GTGCCCGAGGATTATGGAAACCCCCAGACATTAAAGGGAAGCTTATGGTTTGCTATGAAA CTTTGCCGCGGGGCCAGTGCCTCCTCTACAACTGGGAGGAAGAGAGAGCCACCAACCACC TGGATCAAGTCCCAAGCATGCAGGATGGCTCTGAGAGTTTTTTCTTCCGACACGGACACC GGGGACTGCTGACTATGCAACTAAAGTCACCCATGCCCTCCAGCACCACCCAGAAAGACT CGTACCAGCCACCAGGAAACGTCTATTGGCCACTTCGAGGGAAGCGTGAAGCCATGCTGG AGATGCTCCTGCAGCATCAGATCTGTAAAGAGGTGCAGGCAGAACAGGAACCCACAAGGA AGCTCTTCGAGGTTGAGTCTGTGACACACCATGACTACCGAATGGAGCTGGCACAAGCAG GGACTCCTGCCCCAACAAAGCCTCACGACTACCGCCAGGAGCAACCTGAGACCTTCTGGA TACAGAGGGCACCACAGCTGCCGACATGGTGGCCATTGCCCACCCAGGTACCAGCAGCAG AAGACTATCTGACTTGGAAAGAATGGGGGTTTACAGGAGTCCAGGAGGTCCTTTCCGCTC TCCTAAGAGCCACACCTGGTGAATACTCAGTAAACATTTGCGGAATGAATGAACACCCTG TCTGCAGCAGGACATGGACAAATAGGCTCTGCCATCAGGAAATGGGAAGCAAGAAAACGG TAACTCAAGAGGACAGAGGCTGGTGAGGAGGTAAAGCAGGCCCAGTGAAATGGACCTGAC AGCCCAAACTGTCAGAGAGGAGGAAAGCAGGCATTTGATTTTGGTAATTGTGCCAAAGCT ATGTAAGAGAATAAATGCCATTGTTTTAAGGAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC019247 unedited NNNNGGGGTAACGTCAGTATTTGTATACGACTCACTATAGGGCGGCCGCATAACTTCGTA TAGCATACATTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAG AGCGGAGTCTGAAGATGGAGACCAACGGGTCTACGGAGGGATCGCGGTCGCGGTCGCGAT CTTTAGACATACAGCCCAGCTCCGAAGGACTGGGGCCCACTTCGGAACCGTTTCCTTCTT CAGATGACAGTCCCAGGTCGGCCCTGGCAGCTGCAACCGCAGCAGCTGCAGCGGCTGCAT CAGCTGCTGCAGCTACTGCAGCCTTCACCACTGCCAAAGCAGCTGCATTATCTACAAAGA CCCCAGCGCCCTGTTCTGAGTTCATGGAGCCGTCCTCTGACCCCAGCCTTCTTGGGGAGC CCTGTGCGGGACCCGGCTTTACCCACAATATAGCCCATGGGAGTCTTGGCTTTGAGCCCG TCTATGTTTCCTGTATTGCTCAGGACACTTGCACTACAACTGACCATAGTTCTAATCCTG GCCCTGTTCCAGGCTCTAGCTCTGGGCCTGTTCTTGGTTCCAGCTCAGGTGCTGGCCATG GCTCTGGCTCTGGCTCTGGTCCTGGCTGTGGCTCTGTCCCTGGCTCTGGCTCTGGTCCTG GTCCTGGCTCTGGTCCTGGCTCTGGTCCTGGTCATGGCTCTGGCTCTTATCCTGGTCCTG CCTCTGGGCCCTGGTCCAAAAACTGGCCCTTGACTCTGAGCTCAGCCCCTGTTTTCCTCC AGGGTTCAAAAACTTGGGGGCAGATCGGGTCCCCTAACTATTACCCCCGGGAAGTCACCC TTGGCCCTGGGAGCCCCAAG
Restriction Sites	Please inquire
ACCN	BC019247
Insert Size	1667 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019247.1, AAH19247.1
RefSeq Size	1667 bp
Locus ID	26206
Gene Summary	The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq, Jul 2012]

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