TMEM74 (BC033057) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NET36
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC033057 edited AGCGGGGGGCCGAGCGGCGTGCAGAGGCTACAAGTGCCGTAGCTGGTGATTGGGGGACTT TCTCCGGGAACCGTGCCGGGAGAGCGCGCGGTGCTGGAGCCGCACCGGGTGGCCGAAGCA GAAGAGACATGGTCTGGCTATGTTGCCTAGGCTGATCATGAACTCCTGGGCTCAAGCTAT TCTCCAAACCTGGCCTCCCAAAGTCCTGGGATTCCAGCAGAGAAGATTCAAGAAGATTCC AGAAGTGGTCAAGACATTGATGTTAAACATAATGTTTCAGTGAAGGATGTTTGATTCCAA GACCTCTGGAAAAGATTCTTTATCTACAGTTTCTAAGATGGCAGATTTGCAGAACTTAAA AGAATACTGTAAGATTGGGATTGATGCCACCTGAAAGTGAAGAGCCAAACTGGAAACGTC ATAAGTGTCTTAAGAAGTTCGACTTTAAACTAAATTCAAGGCCATTTATGGACAGAATAC CATGGTGGGTGGTGTCATCACTATATTTGATACAGCAAGAGCTTCTACAATATAATTAGT GATTATTGCATTCAATTTAATCAAAGAATAGTTTACCATAACACCAACAGGCCATCATTT TATAGTTATCAGGTGGACCAGAGACCAGAATCTAATTGCGCAGATTCTCTTAATAAAATG TTAGTCTATATGTTTGTTTATTGGCTGGTTGGTCTAAAGTGGTTGGTTTATTCATTTTAG ACATTCTAATTTTTGACTCCTCAATGTATATATTGTGCATTTTACGCCAATGCACTTTCA AATGAAACTGTTGAATTTCCTCTGCAGATTAGTCAGATTATATTTTTCAAAGGGGACCTC AACAATGATACACATTCTGCATGCTCTCCTGTCATGTGACCTTGTCCAATTCTGAACATC AAAAGCTGGAGTGTATTTCTTCACTTCCTTGAATCTGGGAGGCCCTGTGATTATTTTGAC CAATAAAGCACAGGGTTGAAGTGTGATCACATGCAAGAAATGTGATTACCCCAAGATTAC TGCACTGTGAGAAGCCCAAGCCAAGTGGAAAGGCTCTGGACGGTGAGTTGCCATGCAGGC AGAGGCAGGCCAAGGAAAACCATAGCACCTGACAGTGAATGAAAGTCATCTTGGAGTATA TCCTCCAGTCCCACTGCCTCAGCTGACACCATAGGGATAAAAGACAAACCACCCAGTCAA GCTATTCCTGAGTTCCTTACCTCCAAAACCATGAGTACAATAAAATGGATGATTTAAGCA AAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC033057 unedited AGCGTCAAATTTTGTATACGACTCATATGGGCGGCCGCATAACTTCGTATAGCATACATT ATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGGGGCC GAGCGGCGTGCAGAGGCTACAAGTGCCGTAGCTGGTGATTGGGGGACTTTCTCCGGGAAC CGTGCCGGGAGAGCGCGCGGTGCTGGAGCCGCACCGGGTGGCCGAAGCAGAAGAGACATG GTCTGGCTATGTTGCCTAGGCTGATCATGAACTCCTGGGCTCAAGCTATTCTCCAAACCT GGCCTCCCAAAGTCCTGGGATTCCAGCAGAGAAGATTCAAGAAGATTCCAGAAGTGGTCA AGACATTGATGTTAAACATAATGTTTCAGTGAAGGATGTTTGATTCCAAGACCTCTGGAA AAGATTCTTTATCTACAGTTTCTAAGATGGCAGATTTGCAGAACTTAAAAGAATACTGTA AGATTGGGATTGATGCCACCTGAAAGTGAAGAGCCAAACTGGAAACGTCATAAGTGTCTT AAGAAGTTCGACTTTAAACTAAATTCAAGGCCATTTATGGACAGAATACCATGGTGGGTG GTGTCATCACTATATTTGATACAGCAAGAGCTTCTACAATATAATTAGTGATTATTGCAT TCAATTTAATCAAAGAATAGTTTACCATAACACCAACAGGCCATCATTTTATAGTTATCA GGTGGACCAGAGACCAGAATCTAATTGCGCAGATTCTCTTATAAAATGTTAGTCTATATG GTTGGTTATGGGCTGGTTGGTCTAAAGTGGTGGGTTTATTCATTTTAGACATTCTAATTT TTGACTCCTCAATGTATATATTGTGCATTTTACGCCNATGCACCTTTAAATGGAAACTGT GAATTTC
Restriction Sites	Please inquire
ACCN	BC033057
Insert Size	1273 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033057.1, AAH33057.1
RefSeq Size	1273 bp
Locus ID	157753
Protein Families	Transmembrane
Gene Summary	Plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.