Acyl coenzyme A Thioesterase 4 (ACOT4) (BC031799) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	acyl-CoA thioesterase 4; peroxisomal acyl-CoA thioesterase 2B; PTE-Ib; PTE1B; PTE2B
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC031799 edited CCCCAATAACATGGACAACATATCCCTGGAGTACTTCGAAGAAGCCGTATGCTACATGCT TCAACATCCCCAGGTAAAAGGCCCAGGCATTGGGCTTTTGGGCATTTCTCTAGGAGCTGA TATTTGTCTCTCAATGGCCTCATTCTTGAAGAATGTCTCAGCCACAGTTTCCATCAATGG ATCTGGGATCAGTGGGAACACAGCCATCAACTATAAGCACAGTAGCATTCCACCATTGGG CTATGACCTGAGGAGAATCAAGGTAGCTTTCTCAGGCCTCGTGGACATCGTGGATATAAG GAATGCTCTCGTAGGAGGGTACAAGAACCCCAGCATGATTCCAATAGAGAAGGCCCAGGG GCCCATCCTGCTCATTGTTGGTCAGGATGACCATAACTGGAGAAGTGAGTTGTATGCCCA AACAGTCTCTGAACGGTTACAGGCCCATGGAAAGGAAAAACCCCAGATCATCTGTTACCC TGGGACTGGGCATTACATCGAGCCTCCTTACTTCCCCCTGTGCCCAGCTTCCCTTCACAG ATTACTGAACAAACATGTTATATGGGGTGGGGAGCCCAGGGCTCATTCTAAGGCCCAGGA AGATGCCTGGAAGCAAATTCTAGCCTTCTTCTGCAAACACCTGGGAGGTACCCAGAAAAC AGCTGTCCCTAAATTGTAATGCATTTGTCTATTGTTGACATGAGAGATTCAAGATCAGAT TCTAGTGTTCAGTAACCCTATGTGAATCAGATGTCTCCTGGATAACATTAAAGCCATGTC TTTGTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC031799 unedited CGGGATATCGTCGACCCACGCGTCCGCCCCAATAACATGGACAACATATCCCTGGAGTAC TTCGAAGAAGCCGTATGCTACATGCTTCAACATCCCCAGGTAAAAGGCCCAGGCATTGGG CTTTTGGGCATTTCTCTAGGAGCTGATATTTGTCTCTCAATGGCCTCATTCTTGAAGAAT GTCTCAGCCACAGTTTCCATCAATGGATCTGGGATCAGTGGGAACACAGCCATCAACTAT AAGCACAGTAGCATTCCACCATTGGGCTATGACCTGAGGAGAATCAAGGTAGCTTTCTCA GGCCTCGTGGACATCGTGGATATAAGGAATGCTCTCGTAGGAGGGTACAAGAACCCCAGC ATGATTCCAATAGAGAAGGCCCAGGGGCCCATCCTGCTCATTGTTGGTCAGGATGACCAT AACTGGAGAAGTGAGTTGTATGCCCAAACAGTCTCTGAACGGTTACAGGCCCATGGAAAG GAAAAACCCCAGATCATCTGTTACCCTGGGACTGGGCATTACATCGAGCCTCCTTACTTC CCCCTGTGCCCAGCTTCCCTTCACAGATTACTGAACAAACATGTTATATGGGGTGGGGGG AGCCCAGGGCTCATTCTAAGGCCCAGGAAGATGCCTGGAAGCAAATTCTAGCCTTCTTCT GCAAACACCTGGGAGGTACCCAGAAAACAGCTGTCCCTAAATTGTAATGCATTTGTCTAT TGTTGACATGAGAGATTCAAGATCAGATTCTAGTGTTCAGTAACCCTATGTGAATCAGAT GTCTCCTGGGATACATTAAGCCATGTCTTTGTCAT
Restriction Sites	Please inquire
ACCN	BC031799
Insert Size	898 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC031799.1, AAH31799.2
RefSeq Size	898 bp
Locus ID	122970
Protein Pathways	Biosynthesis of unsaturated fatty acids
Gene Summary	Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:16940157). ACOT4 is a peroxisomal succinyl-coenzyme A thioesterase can also hydrolyze glutaryl-CoA and long chain saturated acyl-CoAs (PubMed:16940157).[UniProtKB/Swiss-Prot Function]

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