TSLP (BC016720) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	thymic stromal lymphopoietin
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_138551 edited GGGGGACTCTCGACTTGTGTTCCCCGCTCCTCCCTGACCTTCCTCCCCTCCCCTTTCACT CAATTCTCACCAACTCTTTCTCTCTCTGGTGTTTTCTCCTTTTCTCGTAAACTTTGCCGC CTATGAGCAGCCACATTGCCTTACTGAAATCCAGAGCCTAACCTTCAATCCCACCGCCGG CTGCGCGTCGCTCGCCAAAGAAATGTTCGCCATGAAAACTAAGGCTGCCTTAGCTATCTG GTGCCCAGGCTATTCGGAAACTCAGATAAATGCTACTCAGGCAATGAAGAAGAGGAGAAA AAGGAAAGTCACAACCAATAAATGTCTGGAACAAGTGTCACAATTACAAGGATTGTGGCG TCGCTTCAATCGACCTTTACTGAAACAACAGTAAACCATCTTTATTATGGTCATATTTCA CAGCACCAAAATAAATCATCTTTATTAAGTAGATGAAACATTAACTCTAACTGTGACAAA GAAGACCACAAATAGTTATCTTTTAATTACAGAAGAGTTTCTTAACTTACTTTTGTAAGT TTTTATTGTGTAAGTTTATAATGCAGGGGAAGTACTACTCCTCAAATGTTGAGGGAAGCT TCCATAACATTGATGACTGGCTTCATGGCAGTAATTCTCGGCTGTAGTTGCATAAGCATT GCTCAAGAGGAAAATCCAAAAGTGCAGCAGGAGAACTTTTTTCCCTGAAAAAGGAAAAAT ATTGAACTCAATGATAGCACCTAAACTTACATTTAAAAGACAGACATTCCTTCTACATGT AATGACACTTCTTGTGTTAAACTAAAAATTTACAAGAGAAGAAAGTGAAAGCAAATGGGG TTTCACAAATAGTTGTAAATATAGTGAAGCAATTTGAAATAATTTTCAAGCAAAGTATTG TGAAAGTATTCTAAGCCAAGTTTTAAATATTATCTAACAGACAAGAGTGGTATATACAAG TAGATCCTGAGAAGTACCTTTGTTACAGCTACTATAAATATACATATAAATTATAGAATC TACTTTAATTTATTTTGTGAACACTTTTGAAAATGTACATGTTCCTTTGTAATTGACACT ATATATTTCTTAATAAAATAATTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC016720 unedited GGGGGTTAGGATTTGTNATACGACTTCACTATAGGGCGGCCGCGAATTCGCCATTACGGC CGGGGGACTCTCGACTTGTGTTCCCCGCTCCTCCCTGACCTTCCTCCCCTCCCCTTTCAC TCAATTCTCACCAACTCTTTCTCTCTCTGGTGTTTTCTCCTTTTCTCGTAAACTTTGCCG CCTATGAGCAGCCACATTGCCTTACTGAAATCCAGAGCCTAACCTTCAATCCCACCGCCG GCTGCGCGTCGCTCGCCAAAGAAATGTTCGCCATGAAAACTAAGGCTGCCTTAGCTATCT GGTGCCCAGGCTATTCGGAAACTCAGATAAATGCTACTCAGGCAATGAAGAAGAGGAGAA AAAGGAAAGTCACAACCAATAAATGTCTGGAACAAGTGTCACAATTACAAGGATTGTGGC GTCGCTTCAATCGACCTTTACTGAAACAACAGTAAACCATCTTTATTATGGTCATATTTC ACAGCACCAAAATAAATCATCTTTATTAAGTAGATGAAACATTAACTCTAACTGTGACAA AGAAGACCACAAATAGTTATCTTTTAATTACAGAAGAGTTTCTTAACTTACTTTTGTAAG TTTTTATTGTGTAAGTTTATAATGCAGGGGAAGTACTACTCCTCNANATGTGAGGGAAGC TTCCCATACATTGATGACTGGCTTCATGGCAGTAATTCTCGGCTGTAGTTGCATAAGCAT TGCTCAAGAGGAAAATCCAANAGTGCAGCAGNGAGACCTTTTTTCCCTGGAAAAGGGNAA AATATTGNAGCTCATGATAGCACCTAAACTTACATTNTAAAGAAGACATTCCTCTACATG TAATGACACTTTCTGGGTTAAACTAAAAATTACAAGAAAGAAAGTGAAGCCAATGGGGTT CACCAAATGTTT
Restriction Sites	Please inquire
ACCN	BC016720
Insert Size	1100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016720.1, AAH16720.1
RefSeq Size	1135 bp
Locus ID	85480
Protein Families	Druggable Genome
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
Gene Summary	This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. In addition, the shorter (predominant) isoform is an antimicrobial protein, displaying antibacterial and antifungal activity against B. cereus, E. coli, E. faecalis, S. mitis, S. epidermidis, and C. albicans. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2020]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.