SLD5 (GINS4) (BC027454) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DNA replication complex GINS protein SLD5; GINS complex subunit 4; GINS complex subunit 4 (Sld5 homolog); MGC14799; SLD5; SLD5 homolog
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC027454 edited CTTTGCGAGAGTAGCGCGGGAGCTGAGAAGTTGCAGGGTTGAGTTGGTCCCGGCAAGTCC TTGAGCAGTTTGTTCCTCTGTCTTCCCGCTTCCTGGTGCCCCGACTGCGTCCCCACGAAC GCCCCGTCCTTACCGCCGGCTGCTGTGGAGTGCCTCGCTGTCTCCCAGGTCCCCGAGTGA GCGTCGAATGGGACCTCTGTGGCTAGGGCAGGCCTGTGTGGGTGGCACTGAGAGGGAGGA GGGGAGGGGGATGTTCGCAGTTTCCTTGCGACCCCAGGGACTTTCTTTGTCCCCGCCATC CACCTCCTTAGGCCTTTGGGATCGCCGACTGGTCTAATGTCGCCGGCCCTTGTGGCTCTG GGAAATTAGCATTGATTAATCTTCCTAACAATTTTTGTTTTTATAGAGACAGGATCTCCC TATGTTGCCCAGGCTGGTCTCGAACTCCTGGGCTCAAGGTATCTTCCTGCCTCGGCTTCC CAAAGTGCTGGGATTACAGGCGTGAGCCCGGCCAGCTCCTAATAATGTTAAGTGCTTGCT AGGAGTCAAGCGCTGTGCTGAGCGCAGGGGCTTCCCACGTCATTGCGTCCCCAGTGCTTA GTGCCAGGCACTGAGCAGAGGCGCGATTCGTATTTGAACTAAAACGAACCCCGTCCCTGA GGAACAAGACCTCTACTGGGAAGTCAAAGAAATGAATAGTGATTGTATGGTGTGATGGGT GCTCAGCTCTTGGTGGACAGAAGTAAGGGGGAGGCTTGTTTGAGAAACGGATACCTGACC TAAGCCTTGGAGGAAGAGGCAGACACTGGAAAACTAGAGTCCCTGCGTTCTCTCACCACT TGCTAAAGGGCCTCTACAACCCCAGGAAACCTCAATAAACCTTTCCTTTCTCCAGATAGA TGTCTGTTCTTGCAGATAATTAGAAAACGCATTGCAGAGTATTGGTTCTTTCCCTCTCAT TAGGTTCCTGGTTTCAGAGAAGATGACCGAAGAAGTGGATTTCCTGGGACAGGACTCTGA TGGGGGTAGTGAGGAAGTGGTCCTAACTCCTGCAGAGCTCATTGAAAGATTGGAGCAGGC CTGGATGAATGAAAAGTTTGCCCCTGAGCTGCTGGAGAGCAAGCCTGAGATTGTAGAATG TGTCATGGAACAGCTGGAGCACATGGAAGAAAATCTCAGGAGAGCCAAAAGGGAGGACCT GAAGGTCAGCATCCACCAAATGGAGATGGAGAGGATCCGCTACGTCCTCAGCAGCTACTT GCGGTGTCGCCTCATGAAGGTTTGACGTGGAGATACCTGGAGGGATTGAGACAGCACAGT CTGTAATGCAGACTGAATGTCCTGGGGAAAACTTGGGCTGGGGCCCAGGAGGGTCCAGGA CCTGAAGATGCTGCTAAGAGCCCAATATCCATGTAGCTAAACTGTAAGGAGAGCGAGAGA TTGCTCCACAGAAGCACAGAGGAACGTGTGTGGCCCACTGCGGGGACACCGCACACATTT TTAGTCTGTTCACCTGCCGAGTGAGTGGTTCCCCCTTGCGTTACAGCATGGCAGTGCTAA AGGGAGAGTTGTTGGCATTGGTCAATAGTGAATGCTGACTAGAGGAGAACTTGTTTCCAA AATTATTTTGTCTGTCAGATGGCCAACGTCAGTGTCAAATGAACCAGGATGACTTACATT CACCTACAGCTTTGTGGCTATCTAACTTACAAAAATGAAGTTGCAGGCCAGGCGTGGTGG CTCACACCTGTAATCCCAGCACTTTGGGAGGCTAGGCGGGTGGATCACCTGAGGTCAGGA GTTTGAGACCAGCCTGACCAACAAGGTGAAACCCCATTTCTACTAAAAATAAAAAAATTA GCCAGGCGTGGCGGCAGGCGCCTGTAGTCCCAGCTACTCAGGAGGCTGAGACAGGAGAAT TGCTTGAGCCCAGGAGGTGGAGGTTTCAGTGAGCCAAGATTGTACCACTGCACTCCAGCC TGGGCAACAGAGCGAGACTCCGCCTCAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC027454 unedited GNNCGGCCGCGNAATTCCCGGGGTATCGTCGACCCACGCGTCCGCTTTGCGAGNATAGCG CGGGAGCTGAGAAGTTGCAGGGTTGAGTTGGTCCCGGCAAGTCCTTGAGCAGTTTGTTCC TCTGTCTTCCCGCTTCCTGGTGCCCCGACTGCGTCCCCACGAACGCCCCGTCCTTACCGC CGGCTGCTGTGGAGTGCCTCGCTGTCTCCCAGGTCCCCGAGTGAGCGTCGAATGGGACCT CTGTGGCTAGGGCAGGCCTGTGTGGGTGGCACTGAGAGGGAGGAGGGGAGGGGGATGTTC GCAGTTTCCTTGCGACCCCAGGGACTTTCTTTGTCCCCGCCATCCACCTCCTTAGGCCTT TGGGATCGCCGACTGGTCTAATGTCGCCGGCCCTTGTGGCTCTGGGAAATTAGCATTGAT TAATCTTCCTAACAATTTTTGTTTTTATAGAGACAGGATCTCCCTATGTTGCCCAGGCTG GTCTCGAACTCCTGGGCTCAAGGTATCTTCCTGCCTCGGCTTCCCAAAGTGCTGGGATTA CAGGCGTGAGCCCGGCCAGCTCCTAATAATGTTAAGTGCTTGCTAGGAGTCAAGCGCTGT GCTGAGCGCAGGGGCTTCCCACGTCATTGCGTCCCCAGTGCTTAGTGCCAGGCACTGAGC AGAGGCGCGATTCGTATTTGAACTAANACGAACCCCGTCCCTGAGGAACAAGACCTCTAC TGGGAAGTCAAAGAAATGAATAGTGATTGTATGGTGTGATGGGTGCTCAGCTCTTGGTGG ACAGAAGTAAGGNGGAGCTTTGTTGAGAACGGATACCTGACCTAAGCCTTGGGAGAAGAG CAGACACTGGAAACTAC
Restriction Sites	Please inquire
ACCN	BC027454
Insert Size	2022 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC027454.1, AAH27454.1
RefSeq Size	2022 bp
Locus ID	84296
Gene Summary	The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.[supplied by OMIM, Mar 2008]

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